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Cytochrome P450 17A1 (CYP17A1) (NM_000102) Human Tagged ORF Clone
CAT＃: RC209042
CYP17A1 (Myc-DDK-tagged)-Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1)
ORF Plasmid: tGFP
"NM_000102" in other vectors (6)
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CNY 5,672.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CPT7; CYP17; P450C17; S17AH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209042 representing NM_000102
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGAGCTCGTGGCTCTCTTGCTGCTTACCCTAGCTTATTTGTTTTGGCCCAAGAGAAGGTGCCCTG GTGCCAAGTACCCCAAGAGCCTCCTGTCCCTGCCCCTGGTGGGCAGCCTGCCATTCCTCCCCAGACATGG CCATATGCATAACAACTTCTTCAAGCTGCAGAAAAAATATGGCCCCATCTATTCTGTTCGTATGGGCACC AAGACTACAGTGATTGTCGGCCACCACCAGCTGGCCAAGGAGGTGCTTATTAAGAAGGGCAAGGACTTCT CTGGGCGGCCTCAAATGGCAACTCTAGACATCGCGTCCAACAACCGTAAGGGTATCGCCTTCGCTGACTC TGGCGCACACTGGCAGCTGCATCGAAGGCTGGCGATGGCCACCTTTGCCCTGTTCAAGGATGGCGATCAG AAGCTGGAGAAGATCATTTGTCAGGAAATCAGTACATTGTGTGATATGCTGGCCACCCACAACGGACAGT CCATAGACATCTCCTTTCCTGTCTTCGTGGCGGTAACCAATGTCATCTCCTTGATCTGCTTCAATACCTC CTACAAGAATGGGGACCCTGAGTTGAATGTCATACAGAATTACAATGAAGGCATCATAGACAACCTGAGC AAAGACAGCCTGGTGGACCTAGTCCCCTGGTTGAAGATTTTCCCCAACAAAACCCTGGAAAAATTAAAGA GCCATGTTAAAATACGAAATGATCTGCTGAATAAAATACTTGAAAATTACAAGGAGAAATTCCGGAGTGA CTCTATCACCAACATGCTGGACACACTGATGCAAGCCAAGATGAACTCAGATAATGGCAATGCTGGCCCA GATCAAGACTCAGAGCTGCTTTCAGATAACCACATTCTCACCACCATAGGGGACATCTTTGGGGCTGGCG TGGAGACCACCACCTCTGTGGTTAAATGGACCCTGGCCTTCCTGCTGCACAATCCTCAGGTGAAGAAGAA GCTCTACGAGGAGATTGACCAGAATGTGGGTTTCAGCCGCACACCAACTATCAGTGACCGTAACCGTCTC CTCCTGCTGGAGGCCACCATCCGAGAGGTGCTTCGCCTCAGGCCCGTGGCCCCTATGCTCATCCCCCACA AGGCCAACGTTGACTCCAGCATCGGTGAGTTTGCTGTGGACAAGGGCACAGAAGTTATCATCAATCTGTG GGCGCTGCATCACAATGAGAAGGAGTGGCACCAGCCGGATCAGTTCATGCCTGAGCGTTTCTTGAATCCA GCGGGGACCCAGCTCATCTCACCGTCAGTAAGCTATTTGCCCTTCGGAGCAGGACCTCGCTCCTGTATAG GTGAGATCCTGGCCCGCCAGGAGCTCTTCCTCATCATGGCCTGGCTGCTGCAGAGGTTCGACCTGGAAGT GCCAGATGATGGGCAGCTGCCCTCCCTGGAAGGCATCCCCAAGGTGGTCTTTCTGATCGACTCTTTCAAA GTGAAGATCAAGGTGCGCCAGGCCTGGAGGGAAGCCCAGGCTGAGGGTAGCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209042 representing NM_000102
Red=Cloning site Green=Tags(s) MWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLPRHGHMHNNFFKLQKKYGPIYSVRMGT KTTVIVGHHQLAKEVLIKKGKDFSGRPQMATLDIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQ KLEKIICQEISTLCDMLATHNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLS KDSLVDLVPWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNGNAGP DQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQNVGFSRTPTISDRNRL LLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEVIINLWALHHNEKEWHQPDQFMPERFLNP AGTQLISPSVSYLPFGAGPRSCIGEILARQELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFK VKIKVRQAWREAQAEGST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000102 |
| ORF Size | 1524 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000102.4 |
| RefSeq Size | 1755 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 1586 |
| UniProt ID | P05093 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450 |
| Protein Pathways | C21-Steroid hormone metabolism, Metabolic pathways |
| MW | 57.2 kDa |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CYP17A1 Maintains the Survival of Glioblastomas by Regulating SAR1-Mediated Endoplasmic Reticulum Health and Redox Homeostasis
,null,
Cancers
,PubMed ID 31527549
[Cytochrome P450 17A1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209042L1 | Lenti ORF clone of Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), Myc-DDK-tagged |
CNY 8,072.00 |
|
| RC209042L2 | Lenti ORF clone of Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), mGFP tagged |
CNY 5,890.00 |
|
| RC209042L3 | Lenti ORF clone of Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209042L4 | Lenti ORF clone of Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), mGFP tagged |
CNY 8,072.00 |
|
| RG209042 | CYP17A1 (tGFP-tagged) - Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1) |
CNY 7,272.00 |
|
| SC102224 | CYP17A1 (untagged)-Human cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1) |
CNY 3,792.00 |
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