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FRG1 (NM_004477) Human Tagged ORF Clone
CAT＃: RC208772
FRG1 (Myc-DDK-tagged)-Human FSHD region gene 1 (FRG1)
ORF Plasmid: tGFP
"NM_004477" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FRG1A; FSG1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208772 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGTACTCCTACGTGAAGTCTACCAAGCTCGTGCTCAAGGGAACCAAGACGAAGAGTAAGAAGA AAAAGAGCAAAGATAAGAAAAGAAAAAGAGAAGAAGATGAAGAAACCCAGCTTGATATTGTTGGAATCTG GTGGACAGTAACAAACTTTGGTGAAATTTCAGGAACCATAGCCATTGAAATGGATAAGGGAACCTATATA CATGCACTCGACAATGGTCTTTTTACCCTGGGAGCTCCACACAAAGAAGTTGATGAGGGCCCTAGTCCTC CAGAGCAGTTTACGGCTGTCAAATTATCTGATTCCAGAATCGCCCTGAAGTCTGGCTATGGAAAATATCT TGGTATAAATTCAGATGGACTTGTTGTTGGGCGTTCAGATGCAATTGGACCAAGAGAACAATGGGAACCA GTCTTTCAAAATGGGAAAATGGCTTTGTTGGCCTCAAATAGCTGCTTTATTAGATGCAATGAAGCAGGGG ACATAGAAGCAAAAAGTAAAACAGCAGGAGAAGAAGAAATGATCAAGATTAGATCCTGTGCTGAAAGAGA AACCAAGAAAAAAGATGACATTCCAGAAGAAGACAAAGGAAATGTAAAACAATGTGAAATCAATTATGTA AAGAAATTTCAGAGCTTCCAAGACCACAAACTTAAAATAAGTAAAGAAGACAGTAAAATTCTTAAAAAGG CTCGGAAAGATGGATTTTTGCATGAGACGCTTCTGGACAGGAGAGCCAAATTGAAAGCCGACAGATACTG CAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208772 protein sequence
Red=Cloning site Green=Tags(s) MAEYSYVKSTKLVLKGTKTKSKKKKSKDKKRKREEDEETQLDIVGIWWTVTNFGEISGTIAIEMDKGTYI HALDNGLFTLGAPHKEVDEGPSPPEQFTAVKLSDSRIALKSGYGKYLGINSDGLVVGRSDAIGPREQWEP VFQNGKMALLASNSCFIRCNEAGDIEAKSKTAGEEEMIKIRSCAERETKKKDDIPEEDKGNVKQCEINYV KKFQSFQDHKLKISKEDSKILKKARKDGFLHETLLDRRAKLKADRYCK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004477 |
| ORF Size | 774 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004477.3 |
| RefSeq Size | 1053 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 2483 |
| UniProt ID | Q14331 |
| MW | 29.2 kDa |
| Gene Summary | This gene maps to a location 100 kb centromeric of the repeat units on chromosome 4q35 which are deleted in facioscapulohumeral muscular dystrophy (FSHD). It is evolutionarily conserved and has related sequences on multiple human chromosomes but DNA sequence analysis did not reveal any homology to known genes. In vivo studies demonstrate the encoded protein is localized to the nucleolus. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208772L1 | Lenti ORF clone of Human FSHD region gene 1 (FRG1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208772L2 | Lenti ORF clone of Human FSHD region gene 1 (FRG1), mGFP tagged |
CNY 5,890.00 |
|
| RC208772L3 | Lenti ORF clone of Human FSHD region gene 1 (FRG1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208772L4 | Lenti ORF clone of Human FSHD region gene 1 (FRG1), mGFP tagged |
CNY 5,890.00 |
|
| RG208772 | FRG1 (tGFP-tagged) - Human FSHD region gene 1 (FRG1) |
CNY 4,000.00 |
|
| SC315500 | FRG1 (untagged)-Human FSHD region gene 1 (FRG1) |
CNY 2,400.00 |
