C9orf98 (AK8) (NM_152572) Human Tagged ORF Clone

CAT#: RC208540

AK8 (Myc-DDK-tagged)-Human adenylate kinase 8 (AK8)

ORF Plasmid: DDK tGFP



  "NM_152572" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal antibody to C9orf98 (chromosome 9 open reading frame 98)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AK 8; C9orf98; DDX31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208540 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCCACTATCGCCCCGCACCGTATCCCCCCCGAGATGCCCCAGTACGGGGAGGAGAACCACATCT
TCGAGTTGATGCAGAACATGCTGGAGCAACTCCTGATCCACCAGCCCGAAGATCCCATCCCCTTCATGAT
CCAGCACTTGCATAGAGACAACGACAATGTGCCCAGGATTGTAATATTAGGTCCACCCGCCTCAGGGAAA
ACAACAATAGCAATGTGGCTCTGCAAACATCTGAACAGCAGTCTCCTCACCCTGGAGAACCTGATCTTAA
ATGAGTTTTCCTATACGGCCACCGAAGCCAGAAGGCTTTATCTGCAAAGGAAGACAGTTCCCAGCGCGCT
GCTCGTCCAGCTGATTCAGGAACGCCTGGCTGAAGAGGATTGCATCAAGCAGGGCTGGATTCTGGATGGC
ATCCCTGAGACGCGTGAGCAGGCTCTGAGGATCCAGACCCTGGGGATCACACCCAGACACGTCATTGTGC
TGAGTGCTCCAGACACGGTCCTGATCGAGAGAAACTTGGGGAAGAGAATCGACCCTCAAACTGGAGAGAT
TTATCACACCACCTTTGACTGGCCACCCGAATCTGAAATCCAGAACCGTCTCATGGTGCCAGAGGACATC
TCAGAGCTGGAGACGGCTCAGAAACTGCTGGAGTATCATAGGAACATCGTCAGGGTCATTCCCTCCTACC
CCAAAATCCTCAAAGTCATCAGTGCTGACCAGCCATGTGTGGACGTCTTCTACCAGGCTCTGACCTATGT
CCAAAGCAACCATCGTACTAATGCCCCGTTCACCCCGAGGGTGCTGCTGCTCGGGCCTGTGGGCAGTGGG
AAAAGTCTGCAGGCCGCCCTCCTGGCCCAGAAATACAGGCTTGTCAATGTCTGCTGTGGGCAACTGCTGA
AAGAGGCTGTGGCAGATAGGACCACGTTTGGCGAGCTCATCCAGCCCTTCTTTGAAAAGGAGATGGCAGT
TCCTGACAGCCTCCTCATGAAGGTGCTGAGCCAGCGCCTGGACCAGCAGGACTGCATCCAGAAAGGCTGG
GTGCTACACGGCGTCCCGCGGGACCTCGACCAGGCACACCTGCTGAACCGCCTGGGCTACAATCCCAACA
GGGTGTTTTTCCTGAATGTGCCATTTGATTCCATCATGGAGCGGCTGACTCTGAGAAGAATTGATCCAGT
CACTGGGGAAAGGTACCACCTCATGTACAAGCCACCTCCCACCATGGAGATCCAGGCTCGCCTCCTGCAG
AACCCAAAGGATGCTGAAGAGCAGGTCAAGCTGAAAATGGACCTGTTCTACAGGAACTCAGCTGACTTGG
AGCAGTTGTATGGGTCGGCCATCACCCTCAATGGGGACCAGGACCCATACACAGTCTTCGAATACATCGA
GAGTGGGATCATTAATCCCCTGCCCAAGAAAATCCCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC208540 protein sequence
Red=Cloning site Green=Tags(s)

MDATIAPHRIPPEMPQYGEENHIFELMQNMLEQLLIHQPEDPIPFMIQHLHRDNDNVPRIVILGPPASGK
TTIAMWLCKHLNSSLLTLENLILNEFSYTATEARRLYLQRKTVPSALLVQLIQERLAEEDCIKQGWILDG
IPETREQALRIQTLGITPRHVIVLSAPDTVLIERNLGKRIDPQTGEIYHTTFDWPPESEIQNRLMVPEDI
SELETAQKLLEYHRNIVRVIPSYPKILKVISADQPCVDVFYQALTYVQSNHRTNAPFTPRVLLLGPVGSG
KSLQAALLAQKYRLVNVCCGQLLKEAVADRTTFGELIQPFFEKEMAVPDSLLMKVLSQRLDQQDCIQKGW
VLHGVPRDLDQAHLLNRLGYNPNRVFFLNVPFDSIMERLTLRRIDPVTGERYHLMYKPPPTMEIQARLLQ
NPKDAEEQVKLKMDLFYRNSADLEQLYGSAITLNGDQDPYTVFEYIESGIINPLPKKIP

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_152572
ORF Size 1437 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_152572.3
RefSeq Size 2185 bp
RefSeq ORF 1440 bp
Locus ID 158067
UniProt ID Q96MA6
MW 54.9 kDa
Gene Summary Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has highest activity toward AMP, and weaker activity toward dAMP, CMP and dCMP. Also displays broad nucleoside diphosphate kinase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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