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Synaptotagmin V (SYT5) (NM_003180) Human Tagged ORF Clone
CAT＃: RC208528
SYT5 (Myc-DDK-tagged)-Human synaptotagmin V (SYT5)
ORF Plasmid: tGFP
"NM_003180" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208528 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGGAGCCCCCAACCCCGGGGCCTCCATCGCCCGACACGCCTCCCGACTCCAGTCGCATCAGCC ACGGCCCAGTGCCCCCCTGGGCCCTGGCCACCATCGTGCTGGTCTCAGGCCTCCTCATCTTCAGCTGCTG TTTCTGTCTCTACCGGAAGAGCTGTCGGAGGCGGACAGGCAAGAAGAGCCAGGCCCAAGCCCAGGTCCAC CTTCAGGAAGTGAAGGGGCTGGGCCAGAGTTACATAGACAAGGTGCAGCCAGAAGTAGAGGAGCTGGAGC CAGCACCATCCGGGCCAGGGCAGCAGGTGGCAGACAAGCATGAGCTAGGACAACTGCAGTACTCCCTGGA TTATGACTTCCAGAGTGGCCAGCTGCTGGTGGGCATTCTGCAAGCAATGGGATTGGCAGCCTTGGATCTT GGTGGCTCCTCGGACCCCTATGTGCGGGTCTACCTGCTGCCGGACAAACGGAGGCGGTACGAGACCAAGG TGCATCGGCAGACGCTGAACCCTCACTTTGGGGAGACCTTCGCCTTCAAGGTCCCCTACGTGGAGCTGGG GGGCAGGGTGCTGGTCATGGCGGTGTACGACTTCGACCGCTTCTCTCGCAATGACGCCATCGGGGAGGTG CGGGTCCCTATGAGCTCCGTGGACCTGGGGCGGCCAGTGCAGGCCTGGCGGGAGCTGCAGGCGGCTCCGC GGGAGGAGCAGGAGAAGCTTGGGGACATCTGCTTCTCCCTCCGCTATGTCCCCACGGCCGGGAAGCTCAC CGTCATCGTCCTGGAGGCTAAAAACCTGAAGAAGATGGACGTAGGAGGACTGTCAGATCCATACGTCAAG GTCCACCTGCTGCAGGGCGGCAAAAAGGTGCGGAAGAAGAAAACCACCATCAAGAAGAACACTCTGAACC CCTATTACAACGAAGCTTTCAGCTTCGAGGTGCCCTGTGACCAAGTCCAGAAGGTGCAGGTGGAGCTGAC CGTGCTGGACTACGACAAGCTGGGCAAGAACGAGGCCATCGGGAGGGTGGCCGTGGGGGCGGCCGCCGGC GGGGCTGGCCTGCGGCACTGGGCGGACATGCTGGCCAACCCGCGGCGGCCCATTGCCCAGTGGCACTCGC TGCGGCCCCCGGACCGAGTGAGGCTGCTGCCTGCGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208528 protein sequence
Red=Cloning site Green=Tags(s) MFPEPPTPGPPSPDTPPDSSRISHGPVPPWALATIVLVSGLLIFSCCFCLYRKSCRRRTGKKSQAQAQVH LQEVKGLGQSYIDKVQPEVEELEPAPSGPGQQVADKHELGQLQYSLDYDFQSGQLLVGILQAMGLAALDL GGSSDPYVRVYLLPDKRRRYETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEV RVPMSSVDLGRPVQAWRELQAAPREEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVK VHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAAG GAGLRHWADMLANPRRPIAQWHSLRPPDRVRLLPAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003180 |
| ORF Size | 1158 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003180.3 |
| RefSeq Size | 1853 bp |
| RefSeq ORF | 1161 bp |
| Locus ID | 6861 |
| UniProt ID | O00445 |
| Domains | C2 |
| Protein Families | Secreted Protein, Transmembrane |
| MW | 42.9 kDa |
| Gene Summary | Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208528L3 | Lenti ORF clone of Human synaptotagmin V (SYT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208528L4 | Lenti ORF clone of Human synaptotagmin V (SYT5), mGFP tagged |
CNY 5,890.00 |
|
| RG208528 | SYT5 (tGFP-tagged) - Human synaptotagmin V (SYT5) |
CNY 4,370.00 |
|
| SC118116 | SYT5 (untagged)-Human synaptotagmin V (SYT5) |
CNY 2,950.00 |
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