Hemogen (HEMGN) (NM_018437) Human Tagged ORF Clone

CAT#: RC208367

HEMGN (Myc-DDK-tagged)-Human hemogen (HEMGN), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_018437" in other vectors (7)

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CNY 4,952.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


HEMGN mouse monoclonal antibody, clone OTI2D7 (formerly 2D7)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CT155; EDAG; EDAG-1; NDR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208367 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTTGGGAAAGGACCAATCTCATTTGAAGCACCATCAGACACCTGACCCTCATCAAGAAGAGAACC
ATTCTCCAGAAGTCATTGGAACCTGGAGTTTGAGAAACAGAGAACTACTTAGAAAAAGAAAAGCTGAAGT
GCATGAAAAGGAAACATCACAATGGCTATTTGGAGAACAGAAAAAACGCAAGCAGCAGAGAACAGGAAAA
GGAAATCGAAGAGGCAGAAAGAGACAACAAAACACAGAATTGAAGGTGGAGCCTCAGCCACAGATAGAAA
AGGAAATGGAGAAAGCACTGGCACCTATAGAGAAAAAAACTGAGCCACCTGGGAGCATAACCAAAGTATT
TCCTTCAGTAGCCTCCCCGCAAAAAGTTGTGCCTGAGGAACACTTTTCTGAAATATGTCAAGAAAGTAAC
ATATATCAGGAGAATTTTTCTGAGTACCAAGAAATAGCAGTACAAAACCATTCTTCTGAAACATGCCAAC
ATGTGTCTGAACCTGAAGACCTCTCTCCTAAAATGTACCAAGAAATATCTGTACTTCAAGACAATTCTTC
CAAAATATGCCAAGACATGAAGGAACCTGAAGACAACTCTCCTAACACATGCCAAGTAATATCTGTAATT
CAAGACCATCCTTTCAAAATGTACCAAGATATGGCTAAACGAGAAGATCTGGCTCCTAAAATGTGCCAAG
AAGCTGCTGTACCCAAAATCCTTCCTTGTCCAACATCTGAAGACACAGCTGATCTGGCAGGATGCTCTCT
TCAAGCATATCCAAAACCAGATGTGCCTAAAGGCTATATTCTTGACACAGACCAAAATCCAGCAGAACCA
GAGGAATACAATGAAACAGATCAAGGAATAGCTGAGACAGAAGGCCTTTTTCCTAAAATACAAGAAATAG
CTGAGCCTAAAGACCTTTCTACAAAAACACACCAAGAATCAGCTGAACCTAAATACCTTCCTCATAAAAC
ATGTAACGAAATTATTGTGCCTAAAGCCCCCTCTCATAAAACAATCCAAGAAACACCTCATTCTGAAGAC
TATTCAATTGAAATAAACCAAGAAACTCCTGGGTCTGAAAAATATTCACCTGAAACGTATCAAGAAATAC
CTGGGCTTGAAGAATATTCACCTGAAATATACCAAGAAACATCCCAGCTTGAAGAATATTCACCTGAAAT
ATACCAAGAAACACCGGGGCCTGAAGACCTCTCTACTGAGACATATAAAAATAAGGATGTGCCTAAAGAA
TGCTTTCCAGAACCACACCAAGAAACAGGTGGGCCCCAAGGCCAGGATCCTAAAGCACACCAGGAAGATG
CTAAAGATGCTTATACTTTTCCTCAAGAAATGAAAGAAAAACCCAAAGAAGAGCCAGGAATACCAGCAAT
TCTGAATGAGAGTCATCCAGAAAATGATGTCTATAGTTATGTTTTGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208367 protein sequence
Red=Cloning site Green=Tags(s)

MDLGKDQSHLKHHQTPDPHQEENHSPEVIGTWSLRNRELLRKRKAEVHEKETSQWLFGEQKKRKQQRTGK
GNRRGRKRQQNTELKVEPQPQIEKEMEKALAPIEKKTEPPGSITKVFPSVASPQKVVPEEHFSEICQESN
IYQENFSEYQEIAVQNHSSETCQHVSEPEDLSPKMYQEISVLQDNSSKICQDMKEPEDNSPNTCQVISVI
QDHPFKMYQDMAKREDLAPKMCQEAAVPKILPCPTSEDTADLAGCSLQAYPKPDVPKGYILDTDQNPAEP
EEYNETDQGIAETEGLFPKIQEIAEPKDLSTKTHQESAEPKYLPHKTCNEIIVPKAPSHKTIQETPHSED
YSIEINQETPGSEKYSPETYQEIPGLEEYSPEIYQETSQLEEYSPEIYQETPGPEDLSTETYKNKDVPKE
CFPEPHQETGGPQGQDPKAHQEDAKDAYTFPQEMKEKPKEEPGIPAILNESHPENDVYSYVLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018437
ORF Size 1995 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018437.5
RefSeq Size 2252 bp
RefSeq ORF 1455 bp
Locus ID 55363
UniProt ID Q9BXL5
MW 55.3 kDa
Gene Summary Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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