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ARHGDIG (NM_001176) Human Tagged ORF Clone
CAT＃: RC208277
ARHGDIG (Myc-DDK-tagged)-Human Rho GDP dissociation inhibitor (GDI) gamma (ARHGDIG)
ORF Plasmid: tGFP
"NM_001176" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RHOGDI-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208277 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCCTGGACGCGTGCGAGCTGGGGGCGCAGCTGCTGGAGCTGCTCCGGCTGGCGCTGTGCGCCC GAGTCCTCCTGGCTGACAAGGAGGGTGGGCCGCCGGCAGTGGACGAGGTGTTGGATGAGGCTGTGCCCGA GTACCGGGCGCCGGGGAGGAAGAGCCTCTTGGAGATCCGGCAGCTGGACCCGGACGACAGGAGCCTGGCC AAGTACAAGCGGGTGCTGCTGGGGCCCCTGCCACCGGCCGTGGACCCAAGCCTGCCCAATGTGCAGGTGA CCAGGCTGACACTCCTGTCGGAACAGGCTCCGGGGCCCGTCGTCATGGATCTCACAGGGGACCTGGCTGT TCTGAAGGACCAGGTGTTTGTCCTGAAGGAAGGTGTTGATTACAGAGTGAAGATCTCCTTCAAGGTCCAC AGGGAGATTGTCAGCGGCCTCAAGTGTCTGCACCACACCTACCGCCGGGGCCTGCGCGTGGACAAGACCG TCTACATGGTGGGCAGCTATGGCCCGAGCGCCCAGGAGTATGAGTTTGTGACTCCGGTGGAGGAAGCGCC GAGGGGTGCGCTGGTGCGGGGCCCCTATCTGGTGGTGTCCCTCTTCACCGACGATGACAGGACGCACCAC CTGTCCTGGGAGTGGGGTCTCTGCATCTGCCAGGACTGGAAGGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC208277 protein sequence
Red=Cloning site Green=Tags(s) MLGLDACELGAQLLELLRLALCARVLLADKEGGPPAVDEVLDEAVPEYRAPGRKSLLEIRQLDPDDRSLA KYKRVLLGPLPPAVDPSLPNVQVTRLTLLSEQAPGPVVMDLTGDLAVLKDQVFVLKEGVDYRVKISFKVH REIVSGLKCLHHTYRRGLRVDKTVYMVGSYGPSAQEYEFVTPVEEAPRGALVRGPYLVVSLFTDDDRTHH LSWEWGLCICQDWKD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001176 |
| ORF Size | 675 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001176.4 |
| RefSeq Size | 942 bp |
| RefSeq ORF | 678 bp |
| Locus ID | 398 |
| UniProt ID | Q99819 |
| Protein Families | Druggable Genome |
| MW | 25.1 kDa |
| Gene Summary | The GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activation of GTPases by inhibiting the exchange of GDP for GTP. See ARHGDIB (MIM 602843).[supplied by OMIM, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208277L3 | Lenti ORF clone of Human Rho GDP dissociation inhibitor (GDI) gamma (ARHGDIG), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208277L4 | Lenti ORF clone of Human Rho GDP dissociation inhibitor (GDI) gamma (ARHGDIG), mGFP tagged |
CNY 5,890.00 |
|
| RG208277 | ARHGDIG (tGFP-tagged) - Human Rho GDP dissociation inhibitor (GDI) gamma (ARHGDIG) |
CNY 4,000.00 |
|
| SC126318 | ARHGDIG (untagged)-Human Rho GDP dissociation inhibitor (GDI) gamma (ARHGDIG) |
CNY 3,990.00 |
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