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NEK10 (NM_001031741) Human Tagged ORF Clone
CAT＃: RC208003
NEK10 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10), transcript variant 1
ORF Plasmid: tGFP
"NM_001031741" in other vectors (5)
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CNY 3,656.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CILD44 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208003 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCCTGTACAGCCTGCAGAACCCCCCGAGGTACTGAAGAGTGAGCCGTATGGGGAGAAGGCTGATG TCTGGGCAGTAGGCTGCATCCTTTATCAGATGGCGACTTTGAGTCCCCCCTTCTACAGCACTAACATGCT GTCCTTGGCTACAAAAATAGTGGAGGCGGTATATGAACCAGTGCCAGAAGGTATCTACTCTGAAAAAGTA ACAGACACCATCAGCAGGTGCCTCACTCCTGATGCGGAAGCTCGTCCAGATATTGTAGAAGTCAGTTCGA TGATATCAGATGTCATGATGAAATATTTAGACAACTTATCTACATCCCAGTTGTCCTTGGAAAAGAAGCT AGAACGGGAACGAAGACGCACACAAAGGTATTTTATGGAAGCCAACCGGAACACCGTCACATGTCACCAT GAGCTGGCTGTTCTATCTCACGAGACCTTTGAGAAGGCAAGTTTGAGTAGCAGCAGCAGTGGAGCAGCCA GCCTGAAAAGTGAACTTTCAGAAAGCGCAGACCTGCCCCCTGAAGGCTTCCAGGCCTCCTATGGTAAAGA CGAAGACAGGGCCTGTGACGAAATCCTGTCAGATGATAACTTCAACCTGGAAAATGCTGAGAAAGATACA TATTCAGAGGTAGATGATGAATTGGACATTTCGGATAACTCCAGCAGCTCCAGTTCAAGCCCTCTGAAAG AATCTACATTCAACATTTTAAAGAGAAGTTTTAGTGCTTCAGGAGGAGAAAGACAATCCCAAACAAGGGA CTTCACTGGAGGAACAGGATCAAGACCAAGACCAGCTTTGCTGCCTCTTGACCTGCTTCTGAAAGTGCCA CCCCATATGCTCAGGGCCCACATTAAGGAAATAGAGGCTGAGTTAGTGACAGGGTGGCAGTCCCATAGCC TTCCTGCTGTGATTCTTCGAAATCTCAAAGATCATGCATCAGCAGGAATTGCTGTGTCCCAGAGGAAAGT GCGTCAGATCAGTGATCCTATTCAGCAGATATTAATTCAGCTGCACAAAATAATCTATATCACACAGCTT CCTCCAGCTTTGCACCACAATTTGAAAAGAAGGGTTATAGAGAGATTCAAGAAATCCCTCTTCAGCCAGC AGAGTAACCCTTGTAATTTGAAATCTGAAATTAAAAAGTTATCTCAGGGATCTCCAGAACCGATTGAGCC CAACTTTTTCACAGCAGATTACCATTTATTACATCGTTCATCCGGTGGAAACAGCCTGTCCCCAAATGAC CCTACAGGTTTACCAACCAGCATTGAATTGGAGGAAGGAATAACATATGAACAGATGCAGACTGTGATTG AAGAAGTCCTTGAGGAAAGTGGCTATTACAATTTTACATCTAACAGGTATCATTCCTATCCATGGGGGAC CAAGAATCACCCAACCAAAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208003 protein sequence
Red=Cloning site Green=Tags(s) MVPVQPAEPPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEAVYEPVPEGIYSEKV TDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHH ELAVLSHETFEKASLSSSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDT YSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTRDFTGGTGSRPRPALLPLDLLLKVP PHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQL PPALHHNLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPND PTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFTSNRYHSYPWGTKNHPTKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031741 |
| ORF Size | 1422 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001031741.1, NP_001026911.1 |
| RefSeq Size | 2085 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 152110 |
| UniProt ID | Q6ZWH5 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 53.1 kDa |
| Gene Summary | Plays a role in the cellular response to UV irradiation. Mediates G2/M cell cycle arrest, MEK autoactivation and ERK1/2-signaling pathway activation in response to UV irradiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208003L1 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208003L2 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10), transcript variant 1, mGFP tagged |
CNY 7,130.00 |
|
| RC208003L3 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10), transcript variant 1, Myc-DDK-tagged |
CNY 7,130.00 |
|
| RC208003L4 | Lenti ORF clone of Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10), transcript variant 1, mGFP tagged |
CNY 7,130.00 |
|
| RG208003 | NEK10 (tGFP-tagged) - Human NIMA (never in mitosis gene a)- related kinase 10 (NEK10), transcript variant 1 |
CNY 5,256.00 |
