CHSY1 (NM_014918) Human Tagged ORF Clone
CAT#: RC207899
CHSY1 (Myc-DDK-tagged)-Human chondroitin sulfate synthase 1 (CHSY1)
ORF Plasmid: tGFP
"NM_014918" in other vectors (4)
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CNY 5,872.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CHSY; ChSy-1; CSS1; TPBS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207899 representing NM_014918
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGCGCGGCCGGCGCGCCTGGCTCAGCGTGCTGCTCGGGCTCGTCCTGGGCTTCGTGCTGGCCT CGCGGCTCGTCCTGCCCCGGGCTTCCGAGCTGAAGCGAGCGGGCCCACGGCGCCGCGCCAGCCCCGAGGG CTGCCGGTCCGGGCAGGCGGCGGCTTCCCAGGCCGGCGGGGCGCGCGGCGATGCGCGCGGGGCGCAGCTC TGGCCGCCCGGCTCGGACCCAGATGGCGGCCCGCGCGACAGGAACTTTCTCTTCGTGGGAGTCATGACCG CCCAGAAATACCTGCAGACTCGGGCCGTGGCCGCCTACAGAACATGGTCCAAGACAATTCCTGGGAAAGT TCAGTTCTTCTCAAGTGAGGGTTCTGACACATCTGTACCAATTCCAGTAGTGCCACTACGGGGTGTGGAC GACTCCTACCCGCCCCAGAAGAAGTCCTTCATGATGCTCAAGTACATGCACGACCACTACTTGGACAAGT ATGAATGGTTTATGAGAGCAGATGATGACGTGTACATCAAAGGAGACCGTCTGGAGAACTTCCTGAGGAG TTTGAACAGCAGCGAGCCCCTCTTTCTTGGGCAGACAGGCCTGGGCACCACGGAAGAAATGGGAAAACTG GCCCTGGAGCCTGGTGAGAACTTCTGCATGGGGGGGCCTGGCGTGATCATGAGCCGGGAGGTGCTTCGGA GAATGGTGCCGCACATTGGCAAGTGTCTCCGGGAGATGTACACCACCCATGAGGACGTGGAGGTGGGAAG GTGTGTCCGGAGGTTTGCAGGGGTGCAGTGTGTCTGGTCTTATGAGATGCAGCAGCTTTTTTATGAGAAT TACGAGCAGAACAAAAAGGGGTACATTAGAGATCTCCATAACAGTAAAATTCACCAAGCTATCACATTAC ACCCCAACAAAAACCCACCCTACCAGTACAGGCTCCACAGCTACATGCTGAGCCGCAAGATATCCGAGCT CCGCCATCGCACAATACAGCTGCACCGCGAAATTGTCCTGATGAGCAAATACAGCAACACAGAAATTCAT AAAGAGGACCTCCAGCTGGGAATCCCTCCCTCCTTCATGAGGTTTCAGCCCCGCCAGCGAGAGGAGATTC TGGAATGGGAGTTTCTGACTGGAAAATACTTGTATTCGGCAGTTGACGGCCAGCCCCCTCGAAGAGGAAT GGACTCCGCCCAGAGGGAAGCCTTGGACGACATTGTCATGCAGGTCATGGAGATGATCAATGCCAACGCC AAGACCAGAGGGCGCATCATTGACTTCAAAGAGATCCAGTACGGCTACCGCCGGGTGAACCCCATGTATG GGGCTGAGTACATCCTGGACCTGCTGCTTCTGTACAAAAAGCACAAAGGGAAGAAAATGACGGTCCCTGT GAGGAGGCACGCGTATTTACAGCAGACTTTCAGCAAAATCCAGTTTGTGGAGCATGAGGAGCTGGATGCA CAAGAGTTGGCCAAGAGAATCAATCAGGAATCTGGATCCTTGTCCTTTCTCTCAAACTCCCTGAAGAAGC TCGTCCCCTTTCAGCTCCCTGGGTCGAAGAGTGAGCACAAAGAACCCAAAGATAAAAAGATAAACATACT GATTCCTTTGTCTGGGCGTTTCGACATGTTTGTGAGATTTATGGGAAACTTTGAGAAGACGTGTCTTATC CCCAATCAGAACGTCAAGCTCGTGGTTCTGCTTTTCAATTCTGACTCCAACCCTGACAAGGCCAAACAAG TTGAACTGATGAGAGATTACCGCATTAAGTACCCTAAAGCCGACATGCAGATTTTGCCTGTGTCTGGAGA GTTTTCAAGAGCCCTGGCCCTGGAAGTAGGATCCTCCCAGTTTAACAATGAATCTTTGCTCTTCTTCTGC GACGTCGACCTCGTGTTTACTACAGAATTCCTTCAGCGATGTCGAGCAAATACAGTTCTGGGCCAACAAA TATATTTTCCAATCATCTTCAGCCAGTATGACCCAAAGATTGTTTATAGTGGGAAAGTTCCCAGTGACAA CCATTTTGCCTTTACTCAGAAAACTGGCTTCTGGAGAAACTATGGGTTTGGCATCACGTGTATTTATAAG GGAGATCTTGTCCGAGTGGGTGGCTTTGATGTTTCCATCCAAGGCTGGGGGCTGGAGGATGTGGACCTTT TCAACAAGGTTGTCCAGGCAGGTTTGAAGACGTTTAGGAGCCAGGAAGTAGGAGTAGTCCACGTCCACCA TCCTGTCTTTTGTGATCCCAATCTTGACCCCAAACAGTACAAAATGTGCTTGGGGTCCAAAGCATCGACC TATGGGTCCACCCAGCAGCTGGCTGAGATGTGGCTGGAAAAAAATGATCCAAGTTACAGTAAAAGCAGCA ATAATAATGGCTCAGTGAGGACAGCC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >RC207899 representing NM_014918
Red=Cloning site Green=Tags(s) MAARGRRAWLSVLLGLVLGFVLASRLVLPRASELKRAGPRRRASPEGCRSGQAAASQAGGARGDARGAQL WPPGSDPDGGPRDRNFLFVGVMTAQKYLQTRAVAAYRTWSKTIPGKVQFFSSEGSDTSVPIPVVPLRGVD DSYPPQKKSFMMLKYMHDHYLDKYEWFMRADDDVYIKGDRLENFLRSLNSSEPLFLGQTGLGTTEEMGKL ALEPGENFCMGGPGVIMSREVLRRMVPHIGKCLREMYTTHEDVEVGRCVRRFAGVQCVWSYEMQQLFYEN YEQNKKGYIRDLHNSKIHQAITLHPNKNPPYQYRLHSYMLSRKISELRHRTIQLHREIVLMSKYSNTEIH KEDLQLGIPPSFMRFQPRQREEILEWEFLTGKYLYSAVDGQPPRRGMDSAQREALDDIVMQVMEMINANA KTRGRIIDFKEIQYGYRRVNPMYGAEYILDLLLLYKKHKGKKMTVPVRRHAYLQQTFSKIQFVEHEELDA QELAKRINQESGSLSFLSNSLKKLVPFQLPGSKSEHKEPKDKKINILIPLSGRFDMFVRFMGNFEKTCLI PNQNVKLVVLLFNSDSNPDKAKQVELMRDYRIKYPKADMQILPVSGEFSRALALEVGSSQFNNESLLFFC DVDLVFTTEFLQRCRANTVLGQQIYFPIIFSQYDPKIVYSGKVPSDNHFAFTQKTGFWRNYGFGITCIYK GDLVRVGGFDVSIQGWGLEDVDLFNKVVQAGLKTFRSQEVGVVHVHHPVFCDPNLDPKQYKMCLGSKAST YGSTQQLAEMWLEKNDPSYSKSSNNNGSVRTA TRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014918 |
ORF Size | 2406 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014918.5 |
RefSeq Size | 4567 bp |
RefSeq ORF | 2409 bp |
Locus ID | 22856 |
UniProt ID | Q86X52 |
Protein Families | Transmembrane |
Protein Pathways | Chondroitin sulfate biosynthesis, Metabolic pathways |
MW | 91.8 kDa |
Gene Summary | This gene encodes a member of the chondroitin N-acetylgalactosaminyltransferase family. These enzymes possess dual glucuronyltransferase and galactosaminyltransferase activity and play critical roles in the biosynthesis of chondroitin sulfate, a glycosaminoglycan involved in many biological processes including cell proliferation and morphogenesis. Decreased expression of this gene may play a role in colorectal cancer, and mutations in this gene are a cause of temtamy preaxial brachydactyly syndrome. [provided by RefSeq, Dec 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability
,Liao, WC;Liao, CK;Tseng, TJ;Ho, YJ;Chen, YR;Lin, KH;Lai, TJ;Lan, CT;Wei, KC;Liu, CH;,
Oncogenesis
,PubMed ID 32019907
[CHSY1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207899L3 | Lenti-ORF clone of CHSY1 (Myc-DDK-tagged)-Human chondroitin sulfate synthase 1 (CHSY1) |
CNY 5,890.00 |
|
RC207899L4 | Lenti-ORF clone of CHSY1 (mGFP-tagged)-Human chondroitin sulfate synthase 1 (CHSY1) |
CNY 5,890.00 |
|
RG207899 | CHSY1 (tGFP-tagged) - Human chondroitin sulfate synthase 1 (CHSY1) |
CNY 4,370.00 |
|
SC108820 | CHSY1 (untagged)-Human chondroitin sulfate synthase 1 (CHSY1) |
CNY 5,880.00 |