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BBS7 (NM_018190) Human Tagged ORF Clone
CAT＃: RC207504
BBS7 (Myc-DDK-tagged)-Human Bardet-Biedl syndrome 7 (BBS7), transcript variant 2
ORF Plasmid: tGFP
"NM_018190" in other vectors (4)
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CNY 4,920.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BBS2L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207504 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGATTTTAAACCGAATGGATTATCTGCAGGTGGGAGTAACATCTCAGAAGACTATGAAGCTAA TTCCTGCCTCAAGACACAGAGCTACACAAAAGGTGGTTATTGGAGATCATGATGGGGTAGTTATGTGCTT TGGCATGAAGAAAGGAGAAGCAGCAGCAGTGTTCAAGACTTTACCCGGGCCGAAGATTGCAAGGCTGGAA CTGGGAGGGGTTATCAACACACCTCAGGAGAAAATTTTTATTGCTGCAGCATCTGAGATTAGAGGCTTCA CAAAAAGAGGAAAACAGTTCCTCTCCTTTGAAACAAACCTCACTGAAAGCATTAAAGCTATGCACATATC TGGCTCAGACCTCTTTCTCAGTGCAAGTTACATCTATAACCATTATTGTGACTGCAAAGACCAACATTAT TACCTTTCTGGGGATAAAATCAATGATGTGATCTGCCTTCCAGTGGAAAGATTATCTCGTATCACACCTG TATTGGCCTGCCAGGACAGAGTGCTCAGAGTTTTACAGGGATCTGATGTGATGTATGCAGTTGAAGTTCC TGGACCCCCTACTGTCTTAGCACTACACAATGGAAATGGCGGTGACTCTGGAGAAGACCTTTTGTTTGGG ACATCAGACGGAAAACTTGCGCTTATACAGATTACTACATCCAAACCAGTACGCAAGTGGGAAATTCAAA ATGAGAAAAAGAGAGGAGGTATTTTGTGTATTGACAGCTTTGACATTGTGGGTGATGGGGTTAAAGATTT ACTTGTTGGGAGAGATGACGGAATGGTGGAAGTGTATAGTTTTGATAATGCAAATGAACCTGTTCTACGA TTTGATCAGATGTTGTCTGAAAGCGTCACATCTATCCAGGGTGGTTGTGTAGGAAAAGACAGCTATGATG AAATCGTGGTGTCCACATATTCAGGCTGGGTTACAGGTCTGACAACAGAGCCCATTCATAAGGAAAGTGG ACCAGGAGAAGAACTAAAAATTAATCAGGAGATGCAGAATAAAATTTCTTCCTTACGGAATGAGTTGGAA CATTTGCAGTATAAGGTATTGCAGGAAAGAGAGAATTATCAACAGTCTTCTCAATCAAGCAAAGCAAAAT CAGCAGTACCTTCCTTTGGTATAAATGATAAATTTACACTAAATAAAGATGATGCCAGTTACAGCCTTAT CTTAGAGGTACAGACTGCAATAGATAATGTCTTAATACAGAGTGATGTTCCAATAGATTTACTTGATGTG GATAAAAATTCTGCTGTTGTTAGCTTTAGCAGCTGTGATTCTGAGTCAAACGACAACTTCCTTCTTGCCA CTTATCGGTGCCAGGCAGATACTACAAGGCTGGAACTCAAGATTCGCTCAATTGAAGGCCAGTATGGCAC ACTACAAGCATATGTGACTCCAAGAATTCAACCCAAAACCTGTCAGGTCCGCCAGTACCACATCAAACCT CTTTCACTCCATCAAAGAACTCACTTTATTGATCATGACAGACCCATGAATACACTGACCCTAACAGGCC AGTTCAGTTTTGCTGAAGTTCACTCCTGGGTGGTTTTTTGTCTGCCTGAAGTTCCAGAAAAACCTCCAGC AGGAGAATGTGTGACATTTTACTTTCAGAACACCTTTCTAGATACACAACTTGAAAGTACCTACAGAAAA GGAGAGGGAGTTTTTAAATCTGACAACATTTCTACTATCTCCATCCTAAAAGATGTGCTTTCTAAAGAAG CTACAAAAAGGAAAATTAACCTCAACATATCATACGAGATAAATGAAGTATCAGTCAAACACACTTTAAA GCTAATCCACCCAAAGCTGGAGTACCAGTTGCTTTTGGCTAAGAAAGTGCAGTTAATTGATGCTTTAAAA GAATTACAGATTCATGAGGGAAATACGAACTTTCTGATACCAGAATATCACTGTATTCTAGAAGAGGCAG ATCACCTACAGGAAGAATACAAAAAGCAACCTGCACATCTTGAAAGACTCTATGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207504 protein sequence
Red=Cloning site Green=Tags(s) MDLILNRMDYLQVGVTSQKTMKLIPASRHRATQKVVIGDHDGVVMCFGMKKGEAAAVFKTLPGPKIARLE LGGVINTPQEKIFIAAASEIRGFTKRGKQFLSFETNLTESIKAMHISGSDLFLSASYIYNHYCDCKDQHY YLSGDKINDVICLPVERLSRITPVLACQDRVLRVLQGSDVMYAVEVPGPPTVLALHNGNGGDSGEDLLFG TSDGKLALIQITTSKPVRKWEIQNEKKRGGILCIDSFDIVGDGVKDLLVGRDDGMVEVYSFDNANEPVLR FDQMLSESVTSIQGGCVGKDSYDEIVVSTYSGWVTGLTTEPIHKESGPGEELKINQEMQNKISSLRNELE HLQYKVLQERENYQQSSQSSKAKSAVPSFGINDKFTLNKDDASYSLILEVQTAIDNVLIQSDVPIDLLDV DKNSAVVSFSSCDSESNDNFLLATYRCQADTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKP LSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECVTFYFQNTFLDTQLESTYRK GEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALK ELQIHEGNTNFLIPEYHCILEEADHLQEEYKKQPAHLERLYG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018190 |
| ORF Size | 2016 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018190.3 |
| RefSeq Size | 2625 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 55212 |
| UniProt ID | Q8IWZ6 |
| MW | 75.4 kDa |
| Gene Summary | This gene encodes one of eight proteins that form the BBSome complex containing BBS1, BBS2, BBS4, BBS5, BBS7, BBS8, BBS9 and BBIP10. The BBSome complex is believed to recruit Rab8(GTP) to the primary cilium and promote ciliogenesis. The BBSome complex assembly is mediated by a complex composed of three chaperonin-like BBS proteins (BBS6, BBS10, and BBS12) and CCT/TRiC family chaperonins. Mutations in this gene are implicated in Bardet-Biedl syndrome, a genetic disorder whose symptoms include obesity, retinal degeneration, polydactyly and nephropathy; however, mutations in this gene and the BBS8 gene are thought to play a minor role and mutations in chaperonin-like BBS genes are found to be a major contributor to disease development in a multiethnic Bardet-Biedl syndrome patient population. Two transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207504L3 | Lenti ORF clone of Human Bardet-Biedl syndrome 7 (BBS7), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC207504L4 | Lenti ORF clone of Human Bardet-Biedl syndrome 7 (BBS7), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG207504 | BBS7 (tGFP-tagged) - Human Bardet-Biedl syndrome 7 (BBS7), transcript variant 2 |
CNY 6,370.00 |
|
| SC121909 | BBS7 (untagged)-Human Bardet-Biedl syndrome 7 (BBS7), transcript variant 2 |
CNY 7,220.00 |
