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MAPK4 (NM_002747) Human Tagged ORF Clone
CAT＃: RC207414
MAPK4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 4 (MAPK4)
ORF Plasmid: tGFP
"NM_002747" in other vectors (8)
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CNY 4,368.00
CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ERK-4; ERK4; p63-MAPK; p63MAPK; PRKM4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207414 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGAAGGGTGACTGCATCGCCAGTGTCTATGGGTATGACCTCGGTGGGCGCTTTGTTGACTTCC AACCCCTGGGCTTCGGTGTCAATGGTTTGGTGCTGTCGGCCATGGACAGCCGGGCCTGCCGGAAGGTCGC TGTGAAGAAGATTGCCCTGAGCGATGCCCGCAGCATGAAGCACGCGCTCCGAGAGATCAAGATCATTCGG CGCCTGGACCACGACAACATCGTCAAAGTGTACGAGGTGCTCGGTCCCAAGGGCACTGACCTGCAGGGTG AGCTGTTCAAGTTCAGCGTGGCGTACATCGTCCAGGAGTACATGGAGACCGACCTGGCACGCCTGCTGGA GCAGGGCACGCTGGCAGAAGAGCATGCCAAGCTGTTCATGTACCAGCTGCTCCGCGGGCTCAAGTACATC CACTCCGCCAACGTGCTGCACAGGGACCTGAAGCCCGCCAACATCTTCATCAGCACAGAGGACCTCGTGC TCAAGATTGGGGATTTCGGGTTGGCAAGGATCGTTGATCAGCATTACTCCCACAAGGGTTATCTGTCAGA AGGGTTGGTAACAAAGTGGTACCGTTCCCCACGACTGCTCCTTTCCCCCAATAACTACACCAAAGCCATC GACATGTGGGCCGCCGGCTGCATCCTGTCTGAGATGCTTACGGGGAGAATGCTCTTTGCTGGGGCCCATG AGCTGGAGCAGATGCAACTCATCCTGGAGACCATCCCTGTAATCCGGGAGGAAGACAAGGACGAGCTGCT CAGGGTGATGCCTTCCTTTGTCAGCAGCACCTGGGAGGTGAAGAGGCCTCTGCGCAAGCTGCTCCCTGAA GTGAACAGTGAAGCCATCGACTTTCTGGAGAAGATCCTGACCTTTAACCCCATGGATCGCCTAACAGCTG AGATGGGGCTGCAACACCCCTACATGAGCCCATACTCGTGCCCTGAGGACGAGCCCACCTCACAACACCC CTTCCGCATTGAGGATGAGATCGACGACATCGTGCTGATGGCCGCTAACCAGAGCCAGCTGTCCAACTGG GACACGTGCAGTTCCAGGTACCCTGTGAGCCTGTCGTCGGACCTGGAGTGGCGGCCTGACCGGTGCCAGG ACGCCAGCGAGGTACAGCGCGACCCGCGCGCGGGTTCGGCGCCACTGGCTGAGGACGTGCAGGTGGACCC GCGCAAGGACTCGCACAGCAGCTCCGAGCGCTTCCTAGAGCAGTCGCACTCGTCCATGGAGCGCGCCTTC GAGGCCGACTACGGGCGCTCCTGCGACTACAAGGTGGGGTCGCCGTCCTACCTGGACAAGCTGCTGTGGC GCGACAACAAGCCGCACCACTACTCGGAGCCCAAGCTCATCCTGGACCTGTCGCACTGGAAGCAGGCGGC CGGCGCGCCCCCCACGGCCACGGGGCTGGCGGACACGGGGGCGCGCGAGGACGAGCCGGCCAGCCTCTTC CTGGAGATCGCGCAGTGGGTCAAGAGCACGCAGGGCGGCCCAGAGCACGCCAGCCCGCCCGCCGACGACC CCGAGCGCCGCTTGTCTGCCTCGCCCCCCGGCCGCCCGGCCCCGGTGGACGGCGGCGCCAGCCCCCAGTT CGACCTGGACGTGTTCATCTCCCGCGCCCTGAAGCTCTGCACCAAGCCCGAGGACCTGCCGGACAATAAA CTGGGCGACCTCAATGGTGCGTGCATCCCCGAGCACCCTGGCGACCTCGTGCAGACCGAGGCCTTCTCCA AAGAAAGGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207414 protein sequence
Red=Cloning site Green=Tags(s) MAEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAMDSRACRKVAVKKIALSDARSMKHALREIKIIR RLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGTLAEEHAKLFMYQLLRGLKYI HSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAI DMWAAGCILSEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPE VNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNW DTCSSRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAF EADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTGAREDEPASLF LEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLDVFISRALKLCTKPEDLPDNK LGDLNGACIPEHPGDLVQTEAFSKERW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002747 |
| ORF Size | 1761 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002747.3, NP_002738.2 |
| RefSeq Size | 4736 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 5596 |
| UniProt ID | P31152 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 66 kDa |
| Gene Summary | Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207414L1 | Lenti ORF clone of Human mitogen-activated protein kinase 4 (MAPK4), Myc-DDK-tagged |
CNY 6,768.00 |
|
| RC207414L2 | Lenti ORF clone of Human mitogen-activated protein kinase 4 (MAPK4), mGFP tagged |
CNY 6,460.00 |
|
| RC207414L3 | Lenti ORF clone of Human mitogen-activated protein kinase 4 (MAPK4), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC207414L4 | Lenti ORF clone of Human mitogen-activated protein kinase 4 (MAPK4), mGFP tagged |
CNY 6,460.00 |
|
| RG207414 | MAPK4 (tGFP-tagged) - Human mitogen-activated protein kinase 4 (MAPK4) |
CNY 5,968.00 |
|
| SC118463 | MAPK4 (untagged)-Human mitogen-activated protein kinase 4 (MAPK4) |
CNY 4,152.00 |
|
| SC318009 | MAPK4 (untagged)-Human mitogen-activated protein kinase 4 (MAPK4) |
CNY 7,220.00 |
|
| SC323646 | MAPK4 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase 4 (MAPK4) |
CNY 4,152.00 |
