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PHF13 (NM_153812) Human Tagged ORF Clone
CAT＃: RC207336
PHF13 (Myc-DDK-tagged)-Human PHD finger protein 13 (PHF13)
ORF Plasmid: tGFP
"NM_153812" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PHF5; SPOC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207336 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCTGACTCTTGCGCCGCCGCCTTCCACCCGGAGGAATACTCCCCCAGTTGCGAGAGGCGCAGGA CCGTGGAAGACTTCAACAAATTCTGCACCTTTGTCTTGGCCTATGCTGGCTACATCCCTTATCCGAAGGA GGAACTCCCTTTAAGGAGCAGCCCCAGCCCTGCTAACAGCACTGCTGGTACCATTGACAGCGACGGCTGG GACGCGGGTTTCTCAGACATCGCGTCCTCAGTGCCCTTGCCAGTCTCTGACCGCTGCTTTAGCCACCTGC AGCCTACTCTCTTGCAGCGAGCCAAGCCCAGTAACTTCCTGCTGGACAGAAAGAAAACGGACAAGCTGAA GAAGAAGAAGAAGAGGAAGCGCAGGGACAGTGATGCGCCTGGGAAAGAGGGGTACAGGGGGGGCTTGCTG AAGCTGGAAGCCGCTGACCCCTACGTGGAGACCCCCACGAGTCCCACCTTGCAGGATATCCCCCAGGCTC CCAGCGACCCCTGCTCGGGCTGGGACTCCGATACTCCCTCGAGTGGATCTTGTGCCACTGTGTCACCTGA TCAGGTCAAAGAAATAAAAACTGAAGGCAAACGGACTATCGTCCGGCAGGGAAAGCAGGTGGTGTTCCGA GATGAGGACAGCACTGGCAATGATGAGGACATCATGGTGGACTCAGATGACGATTCCTGGGACCTCGTGA CCTGCTTCTGCATGAAGCCATTTGCCGGCCGCCCCATGATCGAGTGTAATGAGTGCCACACCTGGATTCA CCTGTCCTGTGCGAAAATCCGGAAATCCAATGTTCCAGAAGTGTTTGTCTGCCAAAAGTGCCGGGACTCC AAGTTTGACATCCGCCGTTCCAACCGCTCGCGGACGGGCTCCCGGAAGCTGTTCCTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207336 protein sequence
Red=Cloning site Green=Tags(s) MDSDSCAAAFHPEEYSPSCERRRTVEDFNKFCTFVLAYAGYIPYPKEELPLRSSPSPANSTAGTIDSDGW DAGFSDIASSVPLPVSDRCFSHLQPTLLQRAKPSNFLLDRKKTDKLKKKKKRKRRDSDAPGKEGYRGGLL KLEAADPYVETPTSPTLQDIPQAPSDPCSGWDSDTPSSGSCATVSPDQVKEIKTEGKRTIVRQGKQVVFR DEDSTGNDEDIMVDSDDDSWDLVTCFCMKPFAGRPMIECNECHTWIHLSCAKIRKSNVPEVFVCQKCRDS KFDIRRSNRSRTGSRKLFLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153812 |
| ORF Size | 900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153812.1, NP_722519.1 |
| RefSeq Size | 3670 bp |
| RefSeq ORF | 903 bp |
| Locus ID | 148479 |
| UniProt ID | Q86YI8 |
| Domains | PHD |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 33.6 kDa |
| Gene Summary | Modulates chromatin structure. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207336L3 | Lenti ORF clone of Human PHD finger protein 13 (PHF13), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207336L4 | Lenti ORF clone of Human PHD finger protein 13 (PHF13), mGFP tagged |
CNY 5,890.00 |
|
| RG207336 | PHF13 (tGFP-tagged) - Human PHD finger protein 13 (PHF13) |
CNY 4,000.00 |
|
| SC100527 | PHF13 (untagged)-Human PHD finger protein 13 (PHF13) |
CNY 2,400.00 |
