United States
Germany
Japan
United Kingdom
China
Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone
CAT＃: RC207051
KCNJ12 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12)
ORF Plasmid: tGFP
"NM_021012" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hIRK; hIRK1; hkir2.2x; IRK-2; IRK2; kcnj12x; KCNJN1; Kir2.2; Kir2.2v |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207051 representing NM_021012
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATCGTGTCATCGGAGGAGGACGGGCTGCACCTGGTCA CCATGTCGGGCGCCAACGGCTTCGGCAACGGCAAGGTGCACACGCGGCGCAGGTGCCGCAACCGCTTCGT CAAGAAGAATGGCCAGTGCAACATTGAGTTCGCCAACATGGACGAGAAGTCACAGCGCTACCTGGCTGAC ATGTTCACCACCTGTGTGGACATCCGCTGGCGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTTGCCT CCTGGCTGCTGTTCGGCATCATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTGGAGCCGGCTGAGGG CCGGGGCCGCACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCGGCCTTCCTCTTCTCCATCGAGACG CAGACCACCATCGGCTACGGGCTGCGCTGTGTGACGGAGGAGTGCCCGGTGGCCGTCTTCATGGTGGTGG CCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCAAGGCC CAAGAAGCGGGCACAGACGCTGCTGTTCAGCCACAACGCCGTGGTGGCCCTGCGTGACGGCAAGCTCTGC CTCATGTGGCGTGTGGGTAACCTGCGCAAGAGCCACATTGTGGAGGCCCATGTGCGCGCGCAGCTCATCA AGCCGCGGGTCACCGAGGAGGGCGAGTACATCCCGCTGGACCAGATCGACATCGATGTGGGCTTCGACAA GGGCCTGGACCGCATCTTTCTGGTGTCGCCCATCACCATCTTGCATGAGATTGACGAGGCCAGCCCGCTC TTCGGCATCAGCCGGCAGGACCTGGAGACGGACGACTTTGAGATCGTGGTCATCCTGGAAGGCATGGTGG AGGCCACAGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTT TGAGCCCGTGCTCTTCGAGGAGAAGAACCAGTACAAGATTGACTACTCGCACTTCCACAAGACCTATGAG GTGCCCTCTACGCCCCGCTGCAGTGCGAAGGATCTGGTAGAGAACAAGTTCCTGCTGCCCAGCGCCAACT CCTTCTGCTACGAGAACGAGCTGGCCTTCCTGAGCCGTGACGAGGAGGATGAGGCGGACGGAGACCAGGA CGGCCGAAGCCGGGACGGCCTCAGCCCCCAGGCCAGGCATGACTTTGACAGACTCCAGGCTGGCGGCGGG GTCCTGGAGCAGCGGCCCTACAGACGGGAGTCAGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207051 representing NM_021012
Red=Cloning site Green=Tags(s) MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLAD MFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVMQVHGFMAAFLFSIET QTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLC LMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPL FGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYE VPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGG VLEQRPYRRESEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021012 |
| ORF Size | 1299 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021012.5 |
| RefSeq Size | 5230 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 3768 |
| UniProt ID | Q14500 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 48.8 kDa |
| Gene Summary | This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207051L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC207051L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), mGFP tagged |
CNY 5,890.00 |
|
| RC207051L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207051L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), mGFP tagged |
CNY 5,890.00 |
|
| RG207051 | KCNJ12 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12) |
CNY 7,088.00 |
|
| SC124157 | KCNJ12 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12) |
CNY 5,488.00 |
