CD244 (NM_016382) Human Tagged ORF Clone
CAT#: RC206988
CD244 (Myc-DDK-tagged)-Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1
ORF Plasmid: tGFP
"NM_016382" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2B4; NAIL; NKR2B4; Nmrk; SLAMF4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206988 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGCAAGTGGTCACCCTCATACTCCTCCTGCTCCTCAAGGTGTATCAGGGCAAAGGATGCCAGG GATCAGCTGACCATGTGGTTAGCATCTCGGGAGTGCCTCTTCAGTTACAACCAAACAGCATACAGACGAA GGTTGACAGCATTGCATGGAAGAAGTTGCTGCCCTCACAAAATGGATTTCATCACATATTGAAGTGGGAG AATGGCTCTTTGCCTTCCAATACTTCCAATGATAGATTCAGTTTTATAGTCAAGAACTTGAGTCTTCTCA TCAAGGCAGCTCAGCAGCAGGACAGTGGCCTCTACTGCCTGGAGGTCACCAGTATATCTGGAAAAGTTCA GACAGCCACGTTCCAGGTTTTTGTATTTGATAAAGTTGAGAAACCCCGCCTACAGGGGCAGGGGAAGATC CTGGACAGAGGGAGATGCCAAGTGGCTCTGTCTTGCTTGGTCTCCAGGGATGGCAATGTGTCCTATGCTT GGTACAGAGGGAGCAAGCTGATCCAGACAGCAGGGAACCTCACCTACCTGGACGAGGAGGTTGACATTAA TGGCACTCACACATATACCTGCAATGTCAGCAATCCTGTTAGCTGGGAAAGCCACACCCTGAATCTCACT CAGGACTGTCAGAATGCCCATCAGGAATTCAGATTTTGGCCGTTTTTGGTGATCATCGTGATTCTAAGCG CACTGTTCCTTGGCACCCTTGCCTGCTTCTGTGTGTGGAGGAGAAAGAGGAAGGAGAAGCAGTCAGAGAC CAGTCCCAAGGAATTTTTGACAATTTACGAAGATGTCAAGGATCTGAAAACCAGGAGAAATCACGAGCAG GAGCAGACTTTTCCTGGAGGGGGGAGCACCATCTACTCTATGATCCAGTCCCAGTCTTCTGCTCCCACGT CACAAGAACCTGCATATACATTATATTCATTAATTCAGCCTTCCAGGAAGTCTGGATCCAGGAAGAGGAA CCACAGCCCTTCCTTCAATAGCACTATCTATGAAGTGATTGGAAAGAGTCAACCTAAAGCCCAGAACCCT GCTCGATTGAGCCGCAAAGAGCTGGAGAACTTTGATGTTTATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206988 protein sequence
Red=Cloning site Green=Tags(s) MLGQVVTLILLLLLKVYQGKGCQGSADHVVSISGVPLQLQPNSIQTKVDSIAWKKLLPSQNGFHHILKWE NGSLPSNTSNDRFSFIVKNLSLLIKAAQQQDSGLYCLEVTSISGKVQTATFQVFVFDKVEKPRLQGQGKI LDRGRCQVALSCLVSRDGNVSYAWYRGSKLIQTAGNLTYLDEEVDINGTHTYTCNVSNPVSWESHTLNLT QDCQNAHQEFRFWPFLVIIVILSALFLGTLACFCVWRRKRKEKQSETSPKEFLTIYEDVKDLKTRRNHEQ EQTFPGGGSTIYSMIQSQSSAPTSQEPAYTLYSLIQPSRKSGSRKRNHSPSFNSTIYEVIGKSQPKAQNP ARLSRKELENFDVYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016382 |
ORF Size | 1095 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_016382.4 |
RefSeq Size | 2528 bp |
RefSeq ORF | 1098 bp |
Locus ID | 51744 |
UniProt ID | Q9BZW8 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
Protein Pathways | Natural killer cell mediated cytotoxicity |
MW | 41.1 kDa |
Gene Summary | This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206988L1 | Lenti ORF clone of Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC206988L2 | Lenti ORF clone of Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC206988L3 | Lenti ORF clone of Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC206988L4 | Lenti ORF clone of Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG206988 | CD244 (tGFP-tagged) - Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1 |
CNY 5,256.00 |
|
SC304416 | CD244 (untagged)-Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1 |
CNY 3,656.00 |
|
SC322005 | CD244 (untagged)-Human CD244 molecule, natural killer cell receptor 2B4 (CD244), transcript variant 1 |
CNY 3,656.00 |