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PRAM1 (NM_032152) Human Tagged ORF Clone
CAT＃: RC206972
PRAM1 (Myc-DDK-tagged)-Human PML-RARA regulated adaptor molecule 1 (PRAM1)
ORF Plasmid: tGFP
"NM_032152" in other vectors (6)
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CNY 7,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PML-RAR; PRAM-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206972 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCATCACCTGCCTGCAGCCATGGAGAACCATCAGGACTTCCGGAGCATCAAAGCAAAGTTCCAGG CCTCTCAGCCGGAGCCCAGCGACCTGCCCAAAAAACCTCCGAAGCCTGAGTTTGGTAAACTGAAGAAGTT CTCCCAGCCTGAGCTAAGCGAGCACCCCAAGAAGGCCCCGCTGCCTGAGTTTGGTGCAGTGTCCTTGAAG CCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGAAGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGA AGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAAGAAGCCCCCGCCGCCTGAGGTCACTGACCTCCCCAA GAAGCCGTCCAAACTGGAGTTGAGTGACCTCTCCAAGAAGTTCCCACAGCTGGGGGCCACTCCGTTTCCA AGGAAGCCCCTGCAGCCTGAGGTCGGTGAGGCCCCTTTGAAGGCCTCGCTGCCGGAGCCTGGTGCGCCGG CCCGGAAACCCCTGCAGCCCGACGAACTCAGTCACCCCGCCAGACCCCCCTCCGAACCCAAATCCGGCGC ATTCCCCAGGAAGCTCTGGCAACCCGAGGCCGGTGAGGCTACCCCGAGGTCCCCGCAGCCTGAGTTGAGT ACCTTTCCCAAGAAGCCTGCGCAGCCTGAGTTCAACGTGTACCCCAAAAAGCCTCCGCAGCCTCAGGTCG GTGGCCTCCCTAAGAAGTCCGTGCCGCAGCCTGAGTTCAGCGAGGCCGCTCAGACTCCCCTCTGGAAGCC TCAGTCCAGCGAGCCGAAGCGCGACTCCAGCGCCTTTCCCAAAAAGGCCTCCCAGCCTCCGCTGAGTGAC TTTCCCAAGAAGCCTCCGCAGCCTGAGCTTGGGGACCTCACCAGGACCTCCTCAGAGCCCGAAGTCAGCG TGCTTCCCAAGAGGCCGCGGCCGGCCGAATTCAAAGCGCTCTCCAAGAAGCCCCCGCAGCCCGAGCTGGG CGGCCTCCCCAGGACCTCCTCAGAGCCCGAGTTCAACTCACTCCCCAGGAAGCTGCTGCAGCCGGAGCGC CGGGGGCCACCCCGCAAGTTCTCACAGCCTGAGCCCAGCGCTGTCCTCAAGAGACACCCGCAGCCTGAGT TCTTCGGTGATCTCCCTCGAAAGCCTCCACTCCCCAGCTCCGCTTCCGAGAGCTCACTGCCTGCGGCCGT GGCCGGCTTCAGCTCCCGGCACCCGCTCAGCCCTGGGTTTGGAGCCGCTGGGACACCCCGCTGGAGGTCA GGAGGCCTGGTTCACAGTGGAGGGGCCAGGCCAGGCCTCAGACCCAGCCATCCACCCCGGCGGAGGCCTC TGCCCCCTGCCAGCAGCCTGGGACACCCTCCAGCCAAGCCCCCGCTGCCCCCGGGGCCCGTGGATATGCA GAGCTTTCGGAGACCCTCTGCAGCATCCATAGATCTACGGAGGACCCGCTCGGCCGCTGGGCTCCACTTC CAGGACCGACAGCCTGAAGACATCCCGCAGGTCCCAGATGAGATCTACGAGCTGTATGACGATGTGGAAC CCAGAGATGACTCCAGCCCCAGCCCCAAGGGCAGAGATGAAGCGCCCTCAGTTCAGCAAGCCGCCAGGAG GCCACCACAAGACCCAGCGCTCAGGAAGGAGAAGGATCCCCAGCCACAGCAGTTGCCACCCATGGACCCA AAGTTGCTGAAGCAGCTGAGGAAGGCAGAGAAGGCCGAGAGGGAGTTCCGGAAGAAGTTCAAGTTTGAAG GGGAGATCGTGGTTCACACGAAGATGATGATCGACCCCAACGCTAAGACACGTCGCGGGGGTGGCAAGCA CCTCGGGATCCGGCGCGGGGAGATCCTGGAGGTGATCGAGTTCACCAGCAATGAGGAGATGCTGTGCCGG GACCCCAAAGGCAAATATGGCTACGTGCCCAGAACAGCGCTCCTGCCCCTGGAGACGGAGGTGTACGATG ATGTCGACTTCTGCGATCCCCTGGAAAACCAACCACTCCCCCTGGGACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206972 protein sequence
Red=Cloning site Green=Tags(s) MAHHLPAAMENHQDFRSIKAKFQASQPEPSDLPKKPPKPEFGKLKKFSQPELSEHPKKAPLPEFGAVSLK PPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPPPPEVTDLPKKPSKLELSDLSKKFPQLGATPFP RKPLQPEVGEAPLKASLPEPGAPARKPLQPDELSHPARPPSEPKSGAFPRKLWQPEAGEATPRSPQPELS TFPKKPAQPEFNVYPKKPPQPQVGGLPKKSVPQPEFSEAAQTPLWKPQSSEPKRDSSAFPKKASQPPLSD FPKKPPQPELGDLTRTSSEPEVSVLPKRPRPAEFKALSKKPPQPELGGLPRTSSEPEFNSLPRKLLQPER RGPPRKFSQPEPSAVLKRHPQPEFFGDLPRKPPLPSSASESSLPAAVAGFSSRHPLSPGFGAAGTPRWRS GGLVHSGGARPGLRPSHPPRRRPLPPASSLGHPPAKPPLPPGPVDMQSFRRPSAASIDLRRTRSAAGLHF QDRQPEDIPQVPDEIYELYDDVEPRDDSSPSPKGRDEAPSVQQAARRPPQDPALRKEKDPQPQQLPPMDP KLLKQLRKAEKAEREFRKKFKFEGEIVVHTKMMIDPNAKTRRGGGKHLGIRRGEILEVIEFTSNEEMLCR DPKGKYGYVPRTALLPLETEVYDDVDFCDPLENQPLPLGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032152 |
| ORF Size | 2010 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032152.2 |
| RefSeq Size | 2235 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 84106 |
| UniProt ID | Q96QH2 |
| Protein Families | Druggable Genome |
| MW | 74 kDa |
| Gene Summary | The protein encoded by this gene is similar to FYN binding protein (FYB/SLAP-130), an adaptor protein involved in T cell receptor mediated signaling. This gene is expressed and regulated during normal myelopoiesis. The expression of this gene is induced by retinoic acid and is inhibited by the expression of PML-RARalpha, a fusion protein of promyelocytic leukemia (PML) and the retinoic acid receptor-alpha (RARalpha). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206972L1 | Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), Myc-DDK-tagged |
CNY 9,760.00 |
|
| RC206972L2 | Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), mGFP tagged |
CNY 5,890.00 |
|
| RC206972L3 | Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206972L4 | Lenti ORF clone of Human PML-RARA regulated adaptor molecule 1 (PRAM1), mGFP tagged |
CNY 5,890.00 |
|
| RG206972 | PRAM1 (tGFP-tagged) - Human PML-RARA regulated adaptor molecule 1 (PRAM1) |
CNY 8,960.00 |
|
| SC106058 | PRAM1 (untagged)-Human PML-RARA regulated adaptor molecule 1 (PRAM1) |
CNY 7,220.00 |
