ATP6V1E2 (NM_080653) Human Tagged ORF Clone
CAT#: RC206705
ATP6V1E2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 (ATP6V1E2)
ORF Plasmid: tGFP
"NM_080653" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ATP6E1; ATP6EL2; ATP6V1EL2; VMA4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206705 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGAGTGATGTCGATGTGAAAAAGCAGATTAAGCACATGATGGCTTTCATTGAGCAGGAAGCCA ATGAGAAAGCAGAGGAAATCGATGCCAAGGCTGAGGAAGAGTTTAACATTGAGAAAGGACGCCTCGTGCA AACCCAACGACTGAAGATTATGGAGTATTATGAGAAAAAGGAGAAGCAGATAGAGCAGCAGAAGAAAATC CTGATGTCCACCATGAGGAATCAGGCGAGGCTGAAAGTCCTGAGAGCCCGAAATGACCTCATCTCAGATT TGCTCAGTGAGGCGAAGCTGAGACTCAGCAGGATTGTGGAGGACCCAGAGGTCTACCAGGGGCTGCTGGA TAAACTGGTGCTCCAGGGTCTGCTCCGACTGCTGGAACCTGTGATGATTGTACGCTGCCGGCCACAAGAC CTCCTCCTGGTGGAGGCTGCTGTACAAAAAGCCATCCCCGAGTACATGACAATTTCCCAGAAACATGTGG AGGTCCAGATTGATAAAGAGGCATACCTGGCTGTGAATGCAGCTGGAGGTGTGGAGGTCTACAGTGGCAA TCAGAGAATAAAGGTTTCAAATACCTTGGAAAGCCGACTGGATCTCTCAGCCAAGCAAAAGATGCCAGAA ATACGAATGGCCTTGTTTGGTGCTAACACCAACAGAAAGTTCTTTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206705 protein sequence
Red=Cloning site Green=Tags(s) MALSDVDVKKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKI LMSTMRNQARLKVLRARNDLISDLLSEAKLRLSRIVEDPEVYQGLLDKLVLQGLLRLLEPVMIVRCRPQD LLLVEAAVQKAIPEYMTISQKHVEVQIDKEAYLAVNAAGGVEVYSGNQRIKVSNTLESRLDLSAKQKMPE IRMALFGANTNRKFFI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_080653 |
ORF Size | 678 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080653.3, NP_542384.1 |
RefSeq Size | 2025 bp |
RefSeq ORF | 681 bp |
Locus ID | 90423 |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
MW | 26.1 kDa |
Gene Summary | Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206705L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 (ATP6V1E2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206705L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 (ATP6V1E2), mGFP tagged |
CNY 5,890.00 |
|
RG206705 | ATP6V1E2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 (ATP6V1E2) |
CNY 4,000.00 |
|
SC126158 | ATP6V1E2 (untagged)-Human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 (ATP6V1E2) |
CNY 3,990.00 |
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