RCBTB2 (NM_001268) Human Tagged ORF Clone

CAT#: RC206560

RCBTB2 (Myc-DDK-tagged)-Human regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2 (RCBTB2)

ORF Plasmid: DDK tGFP



  "NM_001268" in other vectors (6)

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CNY 4,104.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal antibody to RCBTB2 (regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CHC1L; RLG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206560 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAAGAACTTCCTCTTTTCTCTGGAGACAGTGGCAAGCCAGTACAGGCTACTCTGTCATCTTTGA
AGATGTTAGATGTGGGAAAGTGGCCAATTTTTTCCCTTTGTTCTGAAGAAGAACTACAGTTAATTCGTCA
GGCTTGTGTCTTTGGCAGTGCTGGCAATGAAGTTTTATACACTACAGTAAATGATGAGATTTTTGTGCTT
GGCACAAACTGCTGTGGCTGTTTGGGGTTAGGTGACGTCCAGAGCACCATTGAACCTCGGAGACTGGATT
CTTTAAATGGCAAAAAAATAGCCTGCCTCAGCTATGGGAGTGGTCCACATATTGTCCTTGCAACAACAGA
AGGAGAAGTCTTTACCTGGGGTCATAATGCTTATAGCCAGCTGGGCAATGGGACAACTAATCATGGTTTA
GTGCCCTGTCATATCTCTACTAATCTGTCAAACAAACAAGTCATTGAAGTTGCCTGTGGGTCTTACCATT
CTTTGGTGCTAACATCTGATGGAGAGGTATTTGCCTGGGGTTATAATAACTCTGGGCAGGTAGGATCTGG
ATCAACAGTTAATCAGCCAATCCCTCGAAGAGTCACTGGCTGCCTACAAAATAAAGTAGTTGTGACCATA
GCATGTGGGCAGATGTGCTGCATGGCAGTAGTAGACACGGGGGAGGTCTATGTCTGGGGTTACAACGGAA
ACGGGCAGCTTGGACTCGGCAACAGTGGCAACCAGCCAACCCCTTGCAGAGTGGCAGCTTTGCAAGGCAT
CCGTGTCCAGAGGGTCGCCTGTGGCTACGCACACACATTAGTATTAACAGATGAAGGCCAAGTGTATGCT
TGGGGCGCCAATTCTTATGGGCAGTTGGGCACTGGCAATAAAAGCAACCAGTCCTATCCTACTCCTGTCA
CTGTGGAAAAGGACAGGATTATCGAGATTGCAGCCTGTCACTCCACACACACGTCTGCGGCCAAGACGCA
GGGTGGGCACGTGTACATGTGGGGCCAGTGCCGGGGTCAGTCCGTGATCCTCCCGCACCTCACCCACTTC
TCCTGCACTGACGACGTGTTTGCCTGCTTTGCCACGCCCGCCGTCACGTGGCGCCTCCTCTCCGTGGAAC
CTGATGACCACCTCACAGTGGCTGAGTCACTGAAGAGGGAATTTGACAACCCGGACACTGCAGACCTGAA
GTTTCTAGTTGATGGAAAGTACATTTATGCACATAAAGTCCTTCTCAAGATTCGATGTGAGCATTTTCGT
TCGTCATTGGAAGATAACGAGGATGATATTGTAGAAATGAGTGAATTTTCATATCCTGTTTACCGGGCCT
TCCTGGAATACCTATACACAGACAGCATCAGCCTTTCTCCTGAGGAGGCAGTAGGACTGCTAGACTTGGC
TACATTTTATAGAGAAAATCGTTTGAAAAAGCTCTGCCAACAAACTATCAAGCAAGGCATCTGCGAGGAG
AATGCCATCGCTCTGCTCTCGGCTGCGGTGAAGTATGATGCACAGGATTTAGAAGAATTCTGCTTCAGGT
TTTGCATAAACCATCTGACTGTAGTAACACAAACATCAGGTTTTGCAGAAATGGACCATGATCTCCTGAA
GAACTTTATCAGCAAAGCAAGCAGAGTTGGAGCCTTTAAAAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206560 protein sequence
Red=Cloning site Green=Tags(s)

MEEELPLFSGDSGKPVQATLSSLKMLDVGKWPIFSLCSEEELQLIRQACVFGSAGNEVLYTTVNDEIFVL
GTNCCGCLGLGDVQSTIEPRRLDSLNGKKIACLSYGSGPHIVLATTEGEVFTWGHNAYSQLGNGTTNHGL
VPCHISTNLSNKQVIEVACGSYHSLVLTSDGEVFAWGYNNSGQVGSGSTVNQPIPRRVTGCLQNKVVVTI
ACGQMCCMAVVDTGEVYVWGYNGNGQLGLGNSGNQPTPCRVAALQGIRVQRVACGYAHTLVLTDEGQVYA
WGANSYGQLGTGNKSNQSYPTPVTVEKDRIIEIAACHSTHTSAAKTQGGHVYMWGQCRGQSVILPHLTHF
SCTDDVFACFATPAVTWRLLSVEPDDHLTVAESLKREFDNPDTADLKFLVDGKYIYAHKVLLKIRCEHFR
SSLEDNEDDIVEMSEFSYPVYRAFLEYLYTDSISLSPEEAVGLLDLATFYRENRLKKLCQQTIKQGICEE
NAIALLSAAVKYDAQDLEEFCFRFCINHLTVVTQTSGFAEMDHDLLKNFISKASRVGAFKN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001268
ORF Size 1653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001268.4
RefSeq Size 3280 bp
RefSeq ORF 1656 bp
Locus ID 1102
UniProt ID O95199
Domains BTB, RCC1
MW 60.3 kDa
Gene Summary This gene encodes a protein containing two C-terminal BTB/POZ domains that is related to regulator of chromosome condensation (RCC). The encoded protein may act as a guanine nucleotide exchange factor. This gene is observed to be lost or underexpressed in prostate cancers. There is a pseudogene of this gene on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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