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EML1 (NM_004434) Human Tagged ORF Clone
CAT＃: RC206372
EML1 (Myc-DDK-tagged)-Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2
ORF Plasmid: tGFP
"NM_004434" in other vectors (5)
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CNY 5,968.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BH; ELP79; EMAP; EMAP-1; EMAPL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206372 representing NM_004434
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGCTTCTCCAGCTACAGCAGCCTGTACGACACGTCCTCGCTGCTCCAGTTCTGCAACGATG ACAGCGCTTCTGCTGCAAGTAGCATGGAGGTGACAGACCGCATTGCTTCACTGGAGCAGAGAGTCCAGAT GCAAGAAGACGACATCCAGCTGCTCAAATCAGCTCTAGCTGATGTGGTTCGGCGGCTGAACATTACTGAG GAACAGCAGGCCGTGCTTAACAGGAAAGGACCTACCAAAGCAAGACCACTGATGCAGACCCTGCCTTTAA GAACCACGGTCAACAATGGCACTGTGTTACCAAAGAAACCTACTGGCTCTCTACCATCCCCCTCCGGGGT CAGGAAAGAAACTGCTGTGCCAGCAACCAAAAGTAACATCAAGAGGACCAGCTCTTCTGAACGAGTGTCT CCTGGGGGTCGAAGGGAAAGCAATGGGGATTCCAGAGGAAACCGGAATCGCACAGGCTCCACCAGCAGCT CTTCCAGTGGCAAAAAGAACAGTGAAAGCAAACCCAAGGAGCCTGTATTCAGTGCAGAAGAAGGCTATGT AAAAATGTTTCTTCGTGGACGCCCTGTTACCATGTACATGCCCAAAGATCAAGTGGATTCTTACAGCTTG GAAGCAAAAGTAGAACTTCCAACCAAGAGACTCAAGCTGGAATGGGTCTATGGGTACAGGGGTCGAGACT GCCGTAACAACCTGTACTTGCTTCCGACGGGAGAGACCGTCTACTTCATCGCATCCGTGGTGGTGTTATA CAACGTGGAGGAGCAACTGCAGAGGCATTACGCTGGCCACAACGATGACGTGAAGTGCCTAGCAGTTCAT CCTGATCGGATCACGATAGCAACAGGACAAGTTGCGGGCACATCGAAGGATGGAAAACAATTGCCCCCAC ATGTGCGCATCTGGGATTCTGTGACATTGAATACTCTCCACGTCATTGGAATAGGTTTTTTTGACCGAGC AGTCACCTGTATTGCATTCTCAAAATCTAATGGAGGAACCAATCTCTGTGCTGTGGATGACTCCAACGAC CATGTGCTCTCTGTATGGGACTGGCAGAAAGAAGAAAAACTAGCAGATGTGAAGTGCTCTAATGAAGCTG TGTTTGCTGCGGATTTCCACCCCACGGACACCAACATCATAGTTACTTGTGGAAAATCACATCTCTACTT TTGGACACTAGAAGGAAGCTCCCTTAATAAGAAGCAAGGATTATTCGAGAAACAAGAAAAGCCAAAGTTT GTCCTCTGTGTGACTTTCTCTGAAAACGGTGACACCATTACTGGAGATTCAAGTGGCAACATCTTAGTAT GGGGAAAAGGTACAAATCGAATAAGCTATGCAGTTCAGGGGGCCCATGAGGGTGGCATTTTTGCACTTTG TATGTTAAGAGATGGCACACTGGTGTCGGGAGGTGGGAAAGACCGAAAGCTCATTTCTTGGAGCGGAAAC TATCAAAAACTTCGTAAAACGGAGATTCCAGAACAGTTTGGTCCAATACGGACAGTGGCCGAGGGGAAAG GCGATGTGATCTTGATTGGCACAACTCGAAACTTTGTCCTGCAGGGCACTCTGTCAGGGGACTTCACACC CATTACTCAGGGTCACACTGATGAGCTCTGGGGACTGGCCATCAATGCCTCAAAATCTCAGTTCTTGACC TGTGGGCATGACAAGCATGCCACTCTCTGGGACGCTGTGGGTCACCGTCCCGTCTGGGACAAAATAATAG AGGATCCAGCTCAGTCTTCTGGTTTTCATCCTTCAGGGTCTGTGGTTGCAGTCGGAACACTCACTGGGAG GTGGTTTGTGTTTGACACAGAAACAAAAGACTTGGTCACCGTTCACACAGATGGAAACGAACAGCTCTCT GTAATGCGATACTCACCAGATGGGAATTTCTTAGCCATAGGCTCACATGACAACTGCATCTATATATATG GCGTTAGTGACAACGGGAGGAAGTACACGCGAGTGGGCAAGTGCTCGGGTCATTCCAGCTTCATTACTCA CCTGGACTGGTCTGTAAACTCACAGTTCCTCGTGTCAAATTCCGGAGACTACGAAATCCTCTACTGGGTT CCCTCTGCCTGTAAGCAAGTCGTAAGTGTGGAAACTACAAGAGACATTGAATGGGCTACCTATACCTGCA CTTTGGGATTCCATGTTTTTGGAGTGTGGCCAGAAGGCTCGGACGGAACCGACATCAATGCCGTCTGTCG GGCCCATGAGAAGAAACTCCTGTCAACAGGCGACGACTTTGGCAAAGTGCACCTCTTCTCATACCCCTGC TCGCAGTTCAGGGCTCCAAGCCACATCTACGGCGGGCACAGCAGCCATGTCACCAATGTCGATTTCCTCT GTGAAGACAGCCACCTCATCTCCACGGGCGGGAAAGACACAAGCATCATGCAGTGGCGCGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206372 representing NM_004434
Red=Cloning site Green=Tags(s) MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITE EQQAVLNRKGPTKARPLMQTLPLRTTVNNGTVLPKKPTGSLPSPSGVRKETAVPATKSNIKRTSSSERVS PGGRRESNGDSRGNRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKDQVDSYSL EAKVELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVH PDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGTNLCAVDDSND HVLSVWDWQKEEKLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGSSLNKKQGLFEKQEKPKF VLCVTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWSGN YQKLRKTEIPEQFGPIRTVAEGKGDVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAINASKSQFLT CGHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLS VMRYSPDGNFLAIGSHDNCIYIYGVSDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWV PSACKQVVSVETTRDIEWATYTCTLGFHVFGVWPEGSDGTDINAVCRAHEKKLLSTGDDFGKVHLFSYPC SQFRAPSHIYGGHSSHVTNVDFLCEDSHLISTGGKDTSIMQWRVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004434 |
| ORF Size | 2445 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004434.2, NP_004425.2 |
| RefSeq Size | 4479 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 2009 |
| UniProt ID | O00423 |
| Domains | WD40, HELP |
| MW | 89.7 kDa |
| Gene Summary | Human echinoderm microtubule-associated protein-like is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206372L3 | Lenti ORF clone of Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC206372L4 | Lenti ORF clone of Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
| RG206372 | EML1 (tGFP-tagged) - Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2 |
CNY 7,568.00 |
|
| SC117349 | EML1 (untagged)-Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2 |
CNY 10,216.00 |
|
| SC318296 | EML1 (untagged)-Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2 |
CNY 7,220.00 |
