DNA Ligase IV (LIG4) (NM_002312) Human Tagged ORF Clone
CAT#: RC206295
LIG4 (Myc-DDK-tagged)-Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1
ORF Plasmid: tGFP
"NM_002312" in other vectors (6)
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CNY 6,672.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LIG4S |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206295 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCTCACAAACTTCACAAACTGTTGCATCTCACGTTCCTTTTGCAGATTTGTGTTCAACTTTAG AACGAATACAGAAAAGTAAAGGACGTGCAGAAAAAATCAGACACTTCAGGGAATTTTTAGATTCTTGGAG AAAATTTCATGATGCTCTTCATAAGAACCACAAAGATGTCACAGACTCTTTTTATCCAGCAATGAGACTA ATTCTTCCTCAGCTAGAAAGAGAGAGAATGGCCTATGGAATTAAAGAAACTATGCTTGCTAAGCTTTATA TTGAGTTGCTTAATTTACCTAGAGATGGAAAAGATGCCCTCAAACTTTTAAACTACAGAACACCCACTGG AACTCATGGAGATGCTGGAGACTTTGCAATGATTGCATATTTTGTGTTGAAGCCAAGATGTTTACAGAAA GGAAGTTTAACCATACAGCAAGTAAACGACCTTTTAGACTCAATTGCCAGCAATAATTCTGCTAAAAGAA AAGACCTAATAAAAAAGAGCCTTCTTCAACTTATAACTCAGAGTTCAGCACTTGAGCAAAAGTGGCTTAT ACGGATGATCATAAAGGATTTAAAGCTTGGTGTTAGTCAGCAAACTATCTTTTCTGTTTTTCATAATGAT GCTGCTGAGTTGCATAATGTCACTACAGATCTGGAAAAAGTCTGTAGGCAACTGCATGATCCTTCTGTAG GACTCAGTGATATTTCTATCACTTTATTTTCTGCATTTAAACCAATGCTAGCTGCTATTGCAGATATTGA GCACATTGAGAAGGATATGAAACATCAGAGTTTCTACATAGAAACCAAGCTAGATGGTGAACGTATGCAA ATGCACAAAGATGGAGATGTATATAAATACTTCTCTCGAAATGGATATAACTACACTGATCAGTTTGGTG CTTCTCCTACTGAAGGTTCTCTTACCCCATTCATTCATAATGCATTCAAAGCAGATATACAAATCTGTAT TCTTGATGGTGAGATGATGGCCTATAATCCTAATACACAAACTTTCATGCAAAAGGGAACTAAGTTTGAT ATTAAAAGAATGGTAGAGGATTCTGATCTGCAAACTTGTTATTGTGTTTTTGATGTATTGATGGTTAATA ATAAAAAGCTAGGGCATGAGACTCTGAGAAAGAGGTATGAGATTCTTAGTAGTATTTTTACACCAATTCC AGGTAGAATAGAAATAGTGCAGAAAACACAAGCTCATACTAAGAATGAAGTAATTGATGCATTGAATGAA GCAATAGATAAAAGAGAAGAGGGAATTATGGTAAAACAACCTCTATCCATCTACAAGCCAGACAAAAGAG GTGAAGGGTGGTTAAAAATTAAACCAGAGTATGTCAGTGGACTAATGGATGAATTGGACATTTTAATTGT TGGAGGATATTGGGGTAAAGGATCACGGGGTGGAATGATGTCTCATTTTCTGTGTGCAGTAGCAGAGAAG CCCCCTCCTGGTGAGAAGCCATCTGTGTTTCATACTCTCTCTCGTGTTGGGTCTGGCTGCACCATGAAAG AACTGTATGATCTGGGTTTGAAATTGGCCAAGTATTGGAAGCCTTTTCATAGAAAAGCTCCACCAAGCAG CATTTTATGTGGAACAGAGAAGCCAGAAGTATACATTGAACCTTGTAATTCTGTCATTGTTCAGATTAAA GCAGCAGAGATCGTACCCAGTGATATGTATAAAACTGGCTGCACCTTGCGTTTTCCACGAATTGAAAAGA TAAGAGATGACAAGGAGTGGCATGAGTGCATGACCCTGGACGACCTAGAACAACTTAGGGGGAAGGCATC TGGTAAGCTCGCATCTAAACACCTTTATATAGGTGGTGATGATGAACCACAAGAAAAAAAGCGGAAAGCT GCCCCAAAGATGAAGAAAGTTATTGGAATTATTGAGCACTTAAAAGCACCTAACCTTACTAACGTTAACA AAATTTCTAATATATTTGAAGATGTAGAGTTTTGTGTTATGAGTGGAACAGATAGCCAGCCAAAGCCTGA CCTGGAGAACAGAATTGCAGAATTTGGTGGTTATATAGTACAAAATCCAGGCCCAGACACGTACTGTGTA ATTGCAGGGTCTGAGAACATCAGAGTGAAAAACATAATTTTGTCAAATAAACATGATGTTGTCAAGCCTG CATGGCTTTTAGAATGTTTTAAGACCAAAAGCTTTGTACCATGGCAGCCTCGCTTTATGATTCATATGTG CCCATCAACCAAAGAACATTTTGCCCGTGAATATGATTGCTATGGTGATAGTTATTTCATTGATACAGAC TTGAACCAACTGAAGGAAGTATTCTCAGGAATTAAAAATTCTAACGAGCAGACTCCTGAAGAAATGGCTT CTCTGATTGCTGATTTAGAATATCGGTATTCCTGGGATTGCTCTCCTCTCAGTATGTTTCGACGCCACAC CGTTTATTTGGACTCGTATGCTGTTATTAATGACCTGAGTACCAAAAATGAGGGGACAAGGTTAGCTATT AAAGCCTTGGAGCTTCGGTTTCATGGAGCAAAAGTAGTTTCTTGTTTAGCTGAGGGAGTGTCTCATGTAA TAATTGGGGAAGATCATAGTCGTGTTGCAGATTTTAAAGCTTTTAGAAGAACTTTTAAGAGAAAGTTTAA AATCCTAAAAGAAAGTTGGGTAACTGATTCAATAGACAAGTGTGAATTACAAGAAGAAAACCAGTATTTG ATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206295 protein sequence
Red=Cloning site Green=Tags(s) MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRL ILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQK GSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHND AAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQ MHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFD IKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNE AIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEK PPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIK AAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKA APKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCV IAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTD LNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAI KALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYL I TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002312 |
ORF Size | 2733 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002312.3, NP_002303.2 |
RefSeq Size | 4115 bp |
RefSeq ORF | 2736 bp |
Locus ID | 3981 |
UniProt ID | P49917 |
Domains | DNA_ligase, BRCT |
Protein Families | Druggable Genome |
Protein Pathways | Non-homologous end-joining |
MW | 104 kDa |
Gene Summary | The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206295L1 | Lenti ORF clone of Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1, Myc-DDK-tagged |
CNY 9,072.00 |
|
RC206295L2 | Lenti ORF clone of Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC206295L3 | Lenti ORF clone of Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206295L4 | Lenti ORF clone of Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1, mGFP tagged |
CNY 9,072.00 |
|
RG206295 | LIG4 (tGFP-tagged) - Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1 |
CNY 8,272.00 |
|
SC118710 | LIG4 (untagged)-Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 1 |
CNY 6,680.00 |
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