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PCDHB16 (NM_020957) Human Tagged ORF Clone
CAT＃: RC206237
PCDHB16 (Myc-DDK-tagged)-Human protocadherin beta 16 (PCDHB16)
ORF Plasmid: tGFP
"NM_020957" in other vectors (4)
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CNY 5,688.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ME1; PCDH-BETA16; PCDH3X; PCDHB8a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206237 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATTGGATGGATGCACAATCGGAGACAAAGGCAAGTCCTTGTTTTCTTTGTTTTGCTGAGCTTGT CTGGGGCGGGCGCCGAGTTGGGGTCCTATTCCGTAGTGGAAGAAACGGAGAGAGGCTCTTTTGTGGCAAA TCTAGGAAAAGACCTGGGGTTGGGGTTGACAGAGATGTCCACCCGCAAGGCCAGGATCATTTCCCAGGGG AACAAACAGCATTTGCAGCTCAAGGCTCAAACTGGGGATTTGCTCATAAATGAGAAGCTAGATCGAGAGG AGCTATGCGGTCCCACTGAGCCTTGCATACTACATTTCCAAGTGTTAATGGAAAACCCTTTAGAAATATT TCAGGCTGAACTGAGGGTGATAGATATAAATGACCATTCTCCCATGTTCACTGAAAAGGAAATGATTCTA AAAATACCGGAAAACAGTCCTCTAGGAACTGAGTTCCCTCTGAATCATGCTTTGGACTTGGACGTAGGAA GCAATAATGTTCAAAACTATAAAATCAGCCCAAGCTCTCATTTCCGGGTTCTAATCCATGAATTCAGAGA TGGCAGGAAATACCCTGAGCTAGTGTTGGATAAAGAGCTGGATCGGGAGGAGGAGCCTCAACTAAGATTA ACCCTGACAGCGCTGGATGGTGGCTCTCCACCGCGATCTGGAACTGCTCAGGTCCGTATTGAAGTGGTGG ACATCAATGATAACGCTCCTGAGTTTGAGCAGCCCATCTACAAAGTGCAGATTCCAGAGAACAGTCCTCT TGGCTCCCTGGTTGCCACCGTCTCCGCCAGGGATTTAGACGGCGGAGCCAATGGAAAAATATCATACACA CTCTTTCAGCCTTCGGAGGATATTAGTAAAACTTTGGAGGTAAATCCTATGACAGGGGAAGTTCGACTGA GAAAGCAAGTAGATTTCGAAATGGTTACGTCTTATGAAGTGCGCATCAAAGCCACAGATGGGGGAGGTCT TTCAGGAAAGTGCACTCTTCTCCTGCAGGTGGTGGACGTGAATGACAATCCCCCACAGGTGACCATGTCT GCACTCACCAGCCCCATCGCAGAGAACTCGCCTGAGATAGTAGTTGCTGTTTTCAGCGTTTCAGATCCTG ACTCCGGAAACAATGGGAAGACGATTTCCTCCATCCAGGAAGACCTTCCCTTTCTTCTAAAACCTTCAGT CAAGAACTTTTACACCTTGGTAACGGAGAGAGCACTCGACAGAGAAGCAAGAGCTGAATATAATATCACC CTCACCGTCACAGATATGGGGACTCCAAGGCTGAAAACGGAGCACAACATAACAGTGCAGATATCAGATG TCAATGATAACGCCCCCACTTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCCCGC CCTGCACATCGGCAGCGTCAGCGCCACAGACAGAGACTCGGGCACCAACGCCCAGGTCACCTACTCGCTG CTGCCGCCCCAAGACCCGCACCTGCCCCTCGCCTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGT TCGCCCTCAGGTCGCTGGACTACGAGGCCCTGCAGGCTTTCGAGTTCCGCGTGGGCGCCACAGACCGCGG CTCCCCCGCGCTGAGCAGAGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTA GTGCTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACTGAGCTGGTGCCCCGGGCGGCCGAGCCGGGCT ACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAATGCCTGGCTGTCGTACCAGCTGCT CAAGGCCACGGAGCCCGGGCTGTTCGGTGTGTGGGCGCACAATGGCGAGGTGCGCACCGCCAGGCTGCTG AGCGAGCGCGACGCAGCCAAGCAGAGGCTGGTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGG CCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTTCCTGCCGCTCCCAGAGGCGGC CCCCGGCCAGACCCAGGCCAACTCGCTCACTGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCGTCGCTC TTCCTCTTTTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCTCGGTGGGCC GCTGCTCGATGCCTGAGGGCCCCTTTCCAGGGCGTCTGGTGGACGTAAGCGGCACCGGGACCCTGTCCCA GAGCTACCAATACGAGGTGTGTCTGACAGGAGGCTCAGAAACAAGTGAGTTCAAGTTCCTGAAGCCGATT ATCCCCAACTTCTCTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206237 protein sequence
Red=Cloning site Green=Tags(s) MEIGWMHNRRQRQVLVFFVLLSLSGAGAELGSYSVVEETERGSFVANLGKDLGLGLTEMSTRKARIISQG NKQHLQLKAQTGDLLINEKLDREELCGPTEPCILHFQVLMENPLEIFQAELRVIDINDHSPMFTEKEMIL KIPENSPLGTEFPLNHALDLDVGSNNVQNYKISPSSHFRVLIHEFRDGRKYPELVLDKELDREEEPQLRL TLTALDGGSPPRSGTAQVRIEVVDINDNAPEFEQPIYKVQIPENSPLGSLVATVSARDLDGGANGKISYT LFQPSEDISKTLEVNPMTGEVRLRKQVDFEMVTSYEVRIKATDGGGLSGKCTLLLQVVDVNDNPPQVTMS ALTSPIAENSPEIVVAVFSVSDPDSGNNGKTISSIQEDLPFLLKPSVKNFYTLVTERALDREARAEYNIT LTVTDMGTPRLKTEHNITVQISDVNDNAPTFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSL LPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSREALVRVLVLDANDNSPL VLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLL SERDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPFLPLPEAAPGQTQANSLTVYLVVALASVSSL FLFSVLLFVAVRLCRRSRAASVGRCSMPEGPFPGRLVDVSGTGTLSQSYQYEVCLTGGSETSEFKFLKPI IPNFSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020957 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020957.3 |
| RefSeq Size | 4549 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 57717 |
| UniProt ID | Q9NRJ7 |
| Domains | CA |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 84.9 kDa |
| Gene Summary | This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206237L3 | Lenti ORF clone of Human protocadherin beta 16 (PCDHB16), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC206237L4 | Lenti ORF clone of Human protocadherin beta 16 (PCDHB16), mGFP tagged |
CNY 7,700.00 |
|
| RG206237 | PCDHB16 (tGFP-tagged) - Human protocadherin beta 16 (PCDHB16) |
CNY 6,370.00 |
|
| SC108036 | PCDHB16 (untagged)-Human protocadherin beta 16 (PCDHB16) |
CNY 5,688.00 |
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