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Kir7.1 (KCNJ13) (NM_002242) Human Tagged ORF Clone
CAT＃: RC206128
KCNJ13 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 1
ORF Plasmid: tGFP
"NM_002242" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KIR1.4; KIR7.1; LCA16; SVD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206128 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCAGTAATTGCAAAGTTATTGCTCCTCTCCTAAGTCAAAGATACCGGAGGATGGTCACCAAGG ATGGCCACAGCACACTTCAAATGGATGGCGCTCAAAGAGGTCTTGCATATCTTCGAGATGCTTGGGGAAT CCTAATGGACATGCGCTGGCGTTGGATGATGTTGGTCTTTTCTGCTTCTTTTGTTGTCCACTGGCTTGTC TTTGCAGTGCTCTGGTATGTTCTGGCTGAGATGAATGGTGATCTGGAACTAGATCATGATGCCCCACCTG AAAACCACACTATCTGTGTCAAGTATATCACCAGTTTCACAGCTGCATTCTCCTTCTCCCTGGAGACACA ACTCACAATTGGTTATGGTACCATGTTCCCCAGTGGTGACTGTCCAAGTGCAATCGCCTTACTTGCCATA CAAATGCTCCTAGGCCTCATGCTAGAGGCTTTTATCACAGGTGCTTTTGTGGCGAAGATTGCCCGGCCAA AAAATCGAGCTTTTTCAATTCGCTTTACTGACATAGCAGTAGTAGCTCACATGGATGGCAAACCTAATCT TATCTTCCAAGTGGCCAACACCCGACCTAGCCCTCTAACCAGTGTCCGGGTCTCAGCTGTACTCTATCAG GAAAGAGAAAATGGCAAACTCTACCAGACCAGTGTGGATTTCCACCTTGATGGCATCAGTTCTGACGAAT GTCCATTCTTCATCTTTCCACTAACGTACTATCACTCCATTACACCATCAAGTCCTCTGGCTACTCTGCT CCAGCATGAAAATCCTTCTCACTTTGAATTAGTTGTATTCCTTTCAGCAATGCAGGAGGGCACTGGAGAA ATATGCCAAAGGAGGACATCCTACCTACAGTCTGAAATCATGTTACATCACTGTTTTGCATCTCTGTTGA CCCGAGGTTCCAAATGTGAATATCAAATCAAGATGGAGAATTTTGACAAGACTGTCCCTGAATTTCCAAC TCCTCTGGTTTCTAAAAGCCCAAACAGGACTGACCTGGATATCCACATCAATGGACAAAGCATTGACAAT TTTCAGATCTCTGAAACAGGACTGACAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206128 protein sequence
Red=Cloning site Green=Tags(s) MDSSNCKVIAPLLSQRYRRMVTKDGHSTLQMDGAQRGLAYLRDAWGILMDMRWRWMMLVFSASFVVHWLV FAVLWYVLAEMNGDLELDHDAPPENHTICVKYITSFTAAFSFSLETQLTIGYGTMFPSGDCPSAIALLAI QMLLGLMLEAFITGAFVAKIARPKNRAFSIRFTDIAVVAHMDGKPNLIFQVANTRPSPLTSVRVSAVLYQ ERENGKLYQTSVDFHLDGISSDECPFFIFPLTYYHSITPSSPLATLLQHENPSHFELVVFLSAMQEGTGE ICQRRTSYLQSEIMLHHCFASLLTRGSKCEYQIKMENFDKTVPEFPTPLVSKSPNRTDLDIHINGQSIDN FQISETGLTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002242 |
| ORF Size | 1080 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002242.2, NP_002233.1 |
| RefSeq Size | 3609 bp |
| RefSeq ORF | 1083 bp |
| Locus ID | 3769 |
| UniProt ID | O60928 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 40.6 kDa |
| Gene Summary | This gene encodes a member of the inwardly rectifying potassium channel family of proteins. Members of this family form ion channel pores that allow potassium ions to pass into a cell. The encoded protein belongs to a subfamily of low signal channel conductance proteins that have a low dependence on potassium concentration. Mutations in this gene are associated with snowflake vitreoretinal degeneration. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206128L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206128L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG206128 | KCNJ13 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 1 |
CNY 5,256.00 |
|
| SC128213 | KCNJ13 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), transcript variant 1 |
CNY 3,656.00 |
