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DPPA4 (NM_018189) Human Tagged ORF Clone
CAT＃: RC205999
DPPA4 (Myc-DDK-tagged)-Human developmental pluripotency associated 4 (DPPA4)
ORF Plasmid: tGFP
"NM_018189" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410091M23Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205999 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGCGAGGCTCCGCTTCTTCTACAAGTATGGAGAAGGCAAAAGGCAAGGAGTGGACCTCCACAGAGA AGTCGAGGGAAGAGGATCAGCAGGCTTCTAATCAACCAAATTCAATTGCTTTGCCAGGAACATCAGCAAA GAGAACCAAAGAAAAAATGTCTGTCAAAGGCAGTAAAGTGCTCTGCCCTAAGAAAAAGGCAGAGCACACT GACAACCCCAGACCTCAGAAGAAGATACCAATCCCTCCATTACCTTCTAAACTGCCACCTGTTAATCTGA TTCACCGGGACATTCTGCGGGCCTGGTGCCAACAATTGAAGCTGAGCTCCAAAGGCCAGAAATTGGATGC ATATAAGCGCCTGTGTGCCTTTGCCTACCCAAATCAAAAGGATTTTCCTAGCACAGCAAAAGAGGCCAAA ATCCGGAAATCATTGCAAAAAAAATTAAAGGTGGAAAAGGGGGAAACGTCCCTGCAAAGTTCTGAGACAC ATCCTCCTGAAGTGGCTCTTCCTCCTGTGGGGGAGCCGCCTGCCCTGGAAAATTCCACTGCTCTCCTTGA GGGAGTTAATACAGTTGTGGTGACAACTTCTGCCCCAGAGGCTTTGCTGGCCTCCTGGGCGAGAATTTCA GCCAGGGCGAGGACACCAGAGGCAGTGGAATCTCCACAAGAGGCCTCTGGTGTCAGGTGGTGTGTGGTCC ATGGGAAAAGTCTCCCTGCAGACACAGATGGTTGGGTTCACCTGCAGTTTCATGCTGGTCAAGCCTGGGT TCCAGAAAAGCAAGAAGGGAGAGTGAGTGCACTCTTCTTGCTTCCTGCCTCCAATTTTCCACCCCCGCAC CTTGAAGACAATATGTTGTGCCCCAAATGTGTTCACAGGAACAAGGTCTTAATAAAAAGCCTCCAATGGG AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205999 protein sequence
Red=Cloning site Green=Tags(s) MLRGSASSTSMEKAKGKEWTSTEKSREEDQQASNQPNSIALPGTSAKRTKEKMSVKGSKVLCPKKKAEHT DNPRPQKKIPIPPLPSKLPPVNLIHRDILRAWCQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAK IRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTSAPEALLASWARIS ARARTPEAVESPQEASGVRWCVVHGKSLPADTDGWVHLQFHAGQAWVPEKQEGRVSALFLLPASNFPPPH LEDNMLCPKCVHRNKVLIKSLQWE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018189 |
| ORF Size | 912 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018189.2, NP_060659.2 |
| RefSeq Size | 2823 bp |
| RefSeq ORF | 915 bp |
| Locus ID | 55211 |
| UniProt ID | Q7L190 |
| MW | 33.5 kDa |
| Gene Summary | This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205999L3 | Lenti ORF clone of Human developmental pluripotency associated 4 (DPPA4), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC205999L4 | Lenti ORF clone of Human developmental pluripotency associated 4 (DPPA4), mGFP tagged |
CNY 5,890.00 |
|
| RG205999 | DPPA4 (tGFP-tagged) - Human developmental pluripotency associated 4 (DPPA4) |
CNY 4,000.00 |
|
| SC126928 | DPPA4 (untagged)-Human developmental pluripotency associated 4 (DPPA4) |
CNY 2,400.00 |
