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EIF4ENIF1 (NM_019843) Human Tagged ORF Clone
CAT＃: RC205985
EIF4ENIF1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1
ORF Plasmid: tGFP
"NM_019843" in other vectors (4)
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CNY 7,216.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4E-T; Clast4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205985 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAGGAGAAGTATGGGTGAAACAGAAAGTGGAGATGCTTTCCTTGACCTGAAGAAGCCTCCTGCCT CCAAATGCCCCCATCGCTATACAAAAGAAGAACTCTTGGATATAAAAGAACTCCCCCATTCCAAACAGAG GCCTTCATGCCTTTCTGAAAAATATGACAGTGATGGTGTCTGGGACCCTGAGAAGTGGCATGCCTCTCTC TACCCAGCTTCAGGGCGGAGCTCACCAGTGGAAAGTCTGAAGAAAGAGTTGGATACAGACCGGCCTTCCC TGGTGCGCAGGATAGTAGATCCACGAGAGCGTGTGAAAGAAGATGACTTAGATGTTGTTCTCAGCCCTCA GAGACGGAGCTTTGGAGGGGGCTGCCACGTGACAGCCGCTGTTAGCTCCCGGCGCTCAGGAAGTCCATTA GAGAAAGATAGTGATGGGCTTCGTCTGCTTGGTGGACGTAGGATTGGCAGTGGGAGGATAATCTCTGCCC GGACCTTTGAGAAGGATCACCGTCTTAGCGATAAGGACCTGCGGGACTTGAGAGACAGAGACCGAGAGAG GGACTTCAAGGACAAGCGTTTCAGGAGAGAGTTTGGAGATAGTAAGCGTGTCTTTGGTGAGCGTAGAAGA AATGATTCTTACACAGAAGAAGAACCAGAGTGGTTCTCTGCTGGACCCACAAGTCAGTCTGAAACCATCG AACTGACTGGCTTTGATGATAAGATACTAGAAGAAGATCACAAAGGGAGAAAAAGAACAAGGCGACGGAC AGCCTCTGTGAAGGAAGGTATAGTAGAGTGCAATGGAGGAGTGGCCGAAGAGGATGAAGTGGAGGTCATC CTTGCACAGGAGCCTGCGGCTGATCAGGAAGTGCCAAGGGATGCTGTCTTGCCTGAGCAGTCCCCAGGAG ACTTTGACTTTAATGAGTTCTTTAACCTTGATAAGGTGCCATGCTTGGCTTCGATGATAGAAGATGTTTT GGGAGAAGGGTCAGTCTCTGCCAGTCGGTTCAGTAGGTGGTTCTCTAACCCGAGCAGATCAGGAAGCCGA TCCAGCAGTCTTGGGTCAACACCACATGAAGAGCTAGAGAGACTTGCAGGTCTGGAGCAAGCCATCCTCT CTCCTGGACAGAACTCGGGGAATTACTTTGCTCCTATACCATTGGAAGACCATGCTGAAAATAAAGTGGA TATTTTAGAAATGCTACAGAAAGCCAAAGTGGATTTGAAACCTCTTCTTTCCAGCCTTTCTGCAAATAAA GAAAAACTTAAAGAAAGCTCACATTCAGGGGTTGTGCTTTCAGTGGAGGAGGTAGAAGCAGGTCTGAAGG GCTTGAAGGTTGACCAGCAAGTGAAGAATTCAACTCCCTTCATGGCAGAACACCTAGAAGAGACCTTGAG TGCCGTAACCAACAATCGACAACTGAAGAAAGACGGAGACATGACTGCGTTCAACAAGCTAGTGAGCACA ATGAAGGCAAGTGGGACTTTGCCTTCTCAGCCCAAAGTCAGCCGAAACCTTGAAAGCCATTTGATGTCCC CTGCTGAGATTCCAGGCCAGCCTGTCCCTAAGAACATCCTGCAGGAACTTCTGGGTCAACCAGTTCAGAG ACCTGCTTCTTCCAATCTTCTGAGTGGCCTTATGGGGAGCTTGGAGCCTACAACATCTTTACTGGGCCAA AGAGCACCCTCTCCTCCCTTGTCACAGGTGTTTCAAACTCGAGCAGCCTCAGCTGACTACCTTCGCCCAA GAATACCATCACCAATTGGTTTCACACCAGGACCACAGCAGCTACTCGGAGATCCATTCCAAGGCATGCG CAAACCCATGAGCCCCATCACAGCCCAGATGAGCCAGCTGGAGTTGCAACAGGCAGCTTTAGAAGGGCTG GCCTTGCCACATGACCTTGCTGTACAGGCAGCAAACTTCTACCAGCCTGGTTTTGGCAAACCACAGGTGG ACAGAACCAGAGATGGATTCAGAAACAGGCAACAGCGAGTGACCAAGTCACCAGCACCCGTGCATCGAGG GAATTCCTCTTCCCCTGCCCCTGCTGCCTCCATCACAAGCATGCTTTCTCCTTCCTTTACCCCTACCTCA GTGATTCGTAAGATGTACGAGAGCAAAGAGAAAAGCAAGGAGGAGCCAGCATCTGGAAAAGCAGCTCTTG GTGACAGTAAAGAGGATACTCAGAAGGCCAGTGAAGAAAACCTCCTGTCATCCAGCTCTGTACCCAGTGC CGATCGAGACTCTTCTCCCACTACAAATTCCAAACTGTCAGCATTACAGAGGTCTTCGTGTTCCACCCCA CTGTCCCAGGCCAACCGTTACACCAAAGAACAAGATTATCGACCTAAAGCAACTGGGAGAAAAACACCCA CCTTGGCATCCCCAGTTCCTACAACACCTTTTCTCCGCCCTGTCCACCAAGTTCCCCTTGTCCCCCATGT CCCTATGGTTAGGCCTGCTCACCGGCTTCACCCAGGGTTGGTACAGAGGATGCTGGCCCAGGGAGTACAT CCACAGCATCTTCCAAGTTTGCTCCAAACTGGTGTGCTTCCTCCTGGGATGGACTTGAGTCATTTACAGG GAATATCTGGCCCCATCCTGGGTCAGCCCTTTTACCCTTTACCTGCTGCTAGTCACCCTCTCTTAAACCC TCGTCCTGGAACACCTCTGCATCTGGCAATGGTGCAACAGCAGCTACAGCGCTCAGTTCTGCATCCTCCA GGCTCTGGTTCCCATGCAGCAGCTGTCAGCGTTCAGACAACCCCTCAGAACGTGCCCAGCCGGTCAGGCC TGCCCCACATGCACTCCCAGCTGGAGCATCGCCCCAGCCAGAGGAGCAGCTCCCCTGTGGGCCTTGCCAA ATGGTTTGGCTCAGATGTGCTACAGCAACCCCTGCCCTCCATGCCCGCCAAAGTTATCAGTGTAGATGAA TTGGAATACCGACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205985 protein sequence
Red=Cloning site Green=Tags(s) MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEELLDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASL YPASGRSSPVESLKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPL EKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRR NDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVI LAQEPAADQEVPRDAVLPEQSPGDFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSR SSSLGSTPHEELERLAGLEQAILSPGQNSGNYFAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANK EKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVST MKASGTLPSQPKVSRNLESHLMSPAEIPGQPVPKNILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQ RAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGPQQLLGDPFQGMRKPMSPITAQMSQLELQQAALEGL ALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQQRVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTS VIRKMYESKEKSKEEPASGKAALGDSKEDTQKASEENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTP LSQANRYTKEQDYRPKATGRKTPTLASPVPTTPFLRPVHQVPLVPHVPMVRPAHRLHPGLVQRMLAQGVH PQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPFYPLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPP GSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDE LEYRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019843 |
| ORF Size | 2955 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019843.4 |
| RefSeq Size | 3704 bp |
| RefSeq ORF | 2958 bp |
| Locus ID | 56478 |
| UniProt ID | Q9NRA8 |
| MW | 108.2 kDa |
| Gene Summary | The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205985L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205985L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG205985 | EIF4ENIF1 (tGFP-tagged) - Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1 |
CNY 8,816.00 |
|
| SC107933 | EIF4ENIF1 (untagged)-Human eukaryotic translation initiation factor 4E nuclear import factor 1 (EIF4ENIF1), transcript variant 1 |
CNY 7,224.00 |
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