United States
Germany
Japan
United Kingdom
China
TMEM47 (NM_031442) Human Tagged ORF Clone
CAT＃: RC205971
TMEM47 (Myc-DDK-tagged)-Human transmembrane protein 47 (TMEM47)
ORF Plasmid: tGFP
"NM_031442" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,600.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BCMP1; TM4SF10; VAB-9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205971 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCGGGCAGCGGCATGGAGGAGGTGCGCGTGTCGGTGCTGACCCCCTTGAAGCTGGTCGGGC TGGTGTGCATCTTCCTGGCGCTGTGTCTGGACCTGGGGGCGGTGCTGAGCCCGGCCTGGGTCACAGCTGA CCACCAGTACTACCTGTCGTTGTGGGAGTCCTGCCGCAAACCCGCCAGCTTGGACATCTGGCACTGCGAG TCCACGCTCAGCAGCGATTGGCAGATTGCTACTCTGGCTTTACTCCTGGGCGGCGCTGCCATCATTCTCA TTGCATTCCTGGTGGGTTTGATTTCTATCTGCGTGGGATCTCGAAGGCGTTTCTATAGACCTGTTGCGGT CATGCTTTTTGCAGCAGTTGTTTTACAGGTTTGCAGCCTGGTCCTTTACCCAATCAAGTTCATTGAAACT GTGAGCTTGAAAATTTACCATGAGTTCAACTGGGGTTATGGCCTGGCCTGGGGTGCAACTATATTTTCGT TTGGGGGTGCCATCCTTTATTGCCTGAACCCTAAGAACTATGAAGACTACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205971 protein sequence
Red=Cloning site Green=Tags(s) MASAGSGMEEVRVSVLTPLKLVGLVCIFLALCLDLGAVLSPAWVTADHQYYLSLWESCRKPASLDIWHCE STLSSDWQIATLALLLGGAAIILIAFLVGLISICVGSRRRFYRPVAVMLFAAVVLQVCSLVLYPIKFIET VSLKIYHEFNWGYGLAWGATIFSFGGAILYCLNPKNYEDYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031442 |
| ORF Size | 543 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031442.2, NP_113630.1 |
| RefSeq Size | 4068 bp |
| RefSeq ORF | 546 bp |
| Locus ID | 83604 |
| UniProt ID | Q9BQJ4 |
| Protein Families | Transmembrane |
| MW | 20 kDa |
| Gene Summary | This gene encodes a member of the PMP22/EMP/claudin protein family. The encoded protein is localized to the ER and the plasma membrane. In dogs, transcripts of this gene exist at high levels in the brain. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205971L1 | Lenti ORF clone of Human transmembrane protein 47 (TMEM47), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205971L2 | Lenti ORF clone of Human transmembrane protein 47 (TMEM47), mGFP tagged |
CNY 6,000.00 |
|
| RC205971L3 | Lenti ORF clone of Human transmembrane protein 47 (TMEM47), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205971L4 | Lenti ORF clone of Human transmembrane protein 47 (TMEM47), mGFP tagged |
CNY 5,890.00 |
|
| RG205971 | TMEM47 (tGFP-tagged) - Human transmembrane protein 47 (TMEM47) |
CNY 5,200.00 |
|
| SC111454 | TMEM47 (untagged)-Human transmembrane protein 47 (TMEM47) |
CNY 2,400.00 |
