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SCIN (NM_033128) Human Tagged ORF Clone
CAT＃: RC205801
SCIN (Myc-DDK-tagged)-Human scinderin (SCIN), transcript variant 2
ORF Plasmid: tGFP
"NM_033128" in other vectors (6)
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CNY 5,240.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205801 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGAGCTATACCACGAAGAGTTCGCCCGGGCGGGCAAGCAGGCGGGGCTGCAGGTCTGGAGGA TTGAGAAGCTGGAGCTGGTGCCCGTGCCCCAGAGCGCTCACGGCGACTTCTACGTCGGGGATGCCTACCT GGTGCTGCACACGGCCAAGACGAGCCGAGGCTTCACCTACCGCCTGCACTTCTGGCTCGGAAAGGAGTGT TCCCAGGATGAAAGCACAGCTGCTGCCATCTTCACTGTTCAGATGGATGACTATTTGGGTGGCAAGCCAG TGCAGAATAGAGAACTTCAAGGATATGAGTCTAATGACTTTGTTAGCTATTTCAAAGGCGGTCTGAAATA CAAGGCTGGAGGCGTGGCATCTGGATTAAATCATGTTCTTACGAACGACCTGACAGCCAAGAGGCTCCTA CATGTGAAGGGTCGTAGAGTGGTGAGAGCCACAGAAGTTCCCCTTAGCTGGGACAGTTTCAACAAGGGTG ACTGCTTCATCATTGACCTTGGCACCGAAATTTATCAGTGGTGTGGTTCCTCGTGCAACAAATATGAACG TCTGAAGGCAAACCAGGTAGCTACTGGCATTCGGTACAATGAAAGGAAAGGAAGGTCTGAACTAATTGTC GTGGAAGAAGGAAGTGAACCCTCAGAACTTATAAAGGTCTTAGGGGAAAAGCCAGAGCTTCCAGATGGAG GTGATGATGATGACATTATAGCAGACATAAGTAACAGGAAAATGGCTAAACTATACATGGTTTCAGATGC AAGTGGCTCCATGAGAGTGACTGTGGTGGCAGAAGAAAACCCCTTCTCAATGGCAATGCTGCTGTCTGAA GAATGCTTTATTTTGGACCACGGGGCTGCCAAACAAATTTTCGTATGGAAAGGTAAAGATGCTAATCCCC AAGAGAGGAAGGCTGCAATGAAGACAGCTGAAGAATTTCTACAGCAAATGAATTATTCCAAGAATACCCA AATTCAAGTTCTTCCAGAAGGAGGTGAAACACCAATCTTCAAACAGTTTTTTAAGGACTGGAGAGATAAA GATCAGAGTGATGGCTTCGGGAAAGTTTATGTCACAGAGAAAGTGGCTCAAATAAAACAAATTCCCTTTG ATGCCTCAAAATTACACAGTTCTCCGCAGATGGCAGCCCAGCACAATATGGTGGATGATGGTTCTGGCAA AGTGGAGATTTGGCGTGTAGAAAACAATGGTAGGATCCAAGTTGACCAAAACTCATATGGTGAATTCTAT GGTGGTGACTGCTACATCATACTCTACACCTATCCCAGAGGACAGATTATCTACACGTGGCAAGGAGCAA ATGCCACACGAGATGAGCTGACAACATCTGCGTTCCTGACTGTTCAGTTGGATCGGTCCCTTGGAGGACA GGCTGTGCAGATCCGAGTCTCCCAAGGCAAAGAGCCTGTTCACCTACTGAGTTTGTTCAAAGACAAACCG CTCATTATTTACAAGAATGGAACATCAAAGAAAGGAGGTCAGGCACCTGCTCCCCCTACACGCCTCTTTC AAGTCCGGAGAAACCTGGCATCTATCACCAGAATTGTGGAGGTTGATGTTGATGCAAATTCACTGAATTC TAACGATGTTTTTGTCCTGAAACTGCCACAAAATAGTGGCTACATCTGGGTAGGAAAAGGTGCTAGCCAG GAGGAGGAGAAAGGAGCAGAGTATGTAGCAAGTGTCCTAAAGTGCAAAACCTTAAGGATCCAAGAAGGCG AGGAGCCAGAGGAGTTCTGGAATTCCCTTGGAGGGAAAAAAGACTACCAGACCTCACCACTACTGGAAAC CCAGGCTGAAGACCATCCACCTCGGCTTTACGGCTGCTCTAACAAAACTGGAAGATTTGTTATTGAAGAG ATTCCAGGAGAGTTCACCCAGGATGATTTAGCTGAAGATGATGTCATGTTACTAGATGCTTGGGAACAGA TATTTATTTGGATTGGCAAAGATGCTAATGAAGTTGAGAAAAAAGAATCTCTGAAGTCTGCCAAAATGTA CCTTGAGACAGACCCTTCTGGAAGAGACAAGAGGACACCAATTGTCATCATAAAACAGGGCCATGAGCCA CCCACATTCACAGGCTGGTTCCTGGGCTGGGATTCCAGCAAGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205801 protein sequence
Red=Cloning site Green=Tags(s) MARELYHEEFARAGKQAGLQVWRIEKLELVPVPQSAHGDFYVGDAYLVLHTAKTSRGFTYRLHFWLGKEC SQDESTAAAIFTVQMDDYLGGKPVQNRELQGYESNDFVSYFKGGLKYKAGGVASGLNHVLTNDLTAKRLL HVKGRRVVRATEVPLSWDSFNKGDCFIIDLGTEIYQWCGSSCNKYERLKANQVATGIRYNERKGRSELIV VEEGSEPSELIKVLGEKPELPDGGDDDDIIADISNRKMAKLYMVSDASGSMRVTVVAEENPFSMAMLLSE ECFILDHGAAKQIFVWKGKDANPQERKAAMKTAEEFLQQMNYSKNTQIQVLPEGGETPIFKQFFKDWRDK DQSDGFGKVYVTEKVAQIKQIPFDASKLHSSPQMAAQHNMVDDGSGKVEIWRVENNGRIQVDQNSYGEFY GGDCYIILYTYPRGQIIYTWQGANATRDELTTSAFLTVQLDRSLGGQAVQIRVSQGKEPVHLLSLFKDKP LIIYKNGTSKKGGQAPAPPTRLFQVRRNLASITRIVEVDVDANSLNSNDVFVLKLPQNSGYIWVGKGASQ EEEKGAEYVASVLKCKTLRIQEGEEPEEFWNSLGGKKDYQTSPLLETQAEDHPPRLYGCSNKTGRFVIEE IPGEFTQDDLAEDDVMLLDAWEQIFIWIGKDANEVEKKESLKSAKMYLETDPSGRDKRTPIVIIKQGHEP PTFTGWFLGWDSSKW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033128 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 2586 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 85477 |
| UniProt ID | Q9Y6U3 |
| Domains | GEL, Gelsolin |
| Protein Pathways | Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton |
| MW | 80.5 kDa |
| Gene Summary | SCIN is a Ca(2+)-dependent actin-severing and -capping protein (Zunino et al., 2001 [PubMed 11568009]).[supplied by OMIM, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205801L1 | Lenti ORF clone of Human scinderin (SCIN), transcript variant 2, Myc-DDK-tagged |
CNY 7,640.00 |
|
| RC205801L2 | Lenti ORF clone of Human scinderin (SCIN), transcript variant 2, mGFP tagged |
CNY 7,640.00 |
|
| RC205801L3 | Lenti ORF clone of Human scinderin (SCIN), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205801L4 | Lenti ORF clone of Human scinderin (SCIN), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205801 | SCIN (tGFP-tagged) - Human scinderin (SCIN), transcript variant 2 |
CNY 6,840.00 |
|
| SC101747 | SCIN (untagged)-Human scinderin (SCIN), transcript variant 2 |
CNY 3,656.00 |
