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ZNF259 (ZPR1) (NM_003904) Human Tagged ORF Clone
CAT＃: RC205721
ZNF259 (Myc-DDK-tagged)-Human zinc finger protein 259 (ZNF259)
ORF Plasmid: tGFP
"NM_003904" in other vectors (5)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GKAF; ZNF259 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205721 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCAGCGGGGCTGTGGAACCAGGGCCCCCGGGGGCTGCCGTCGCCCCGTCGCCCGCCCCGGCCC CGCCGCCTGCCCCTGATCACCTGTTCCGGCCCATCAGCGCCGAGGACGAGGAGCAGCAGCCCACCGAGAT CGAGTCGCTATGCATGAACTGTTACTGCAATGGCATGACGCGCCTCCTGCTCACCAAGATTCCCTTCTTC AGAGAAATAATAGTGAGCTCCTTTTCCTGCGAGCACTGTGGCTGGAACAACACGGAGATCCAGTCGGCAG GCAGGATCCAGGACCAGGGAGTGCGCTACACTTTGTCTGTCAGGGCTCTGGAGGACATGAACAGAGAAGT GGTGAAGACTGACTCTGCTGCCACAAGGATTCCTGAGCTAGATTTTGAAATTCCTGCCTTTAGCCAGAAA GGAGCTCTGACCACTGTTGAAGGATTGATCACCCGTGCTATCTCTGGCCTGGAGCAGGACCAGCCTGCAC GAAGGGCAAACAAAGATGCTACAGCTGAAAGAATTGATGAGTTCATTGTCAAACTGAAGGAGCTAAAGCA AGTAGCCTCCCCTTTCACTCTGATCATTGATGATCCCTCAGGGAACAGTTTTGTGGAAAACCCACATGCT CCTCAGAAAGATGATGCCCTGGTGATCACACACTACAACCGGACCCGACAGCAGGAAGAGATGCTGGGGC TTCAAGAAGAAGCACCAGCAGAGAAGCCAGAAGAGGAAGATCTCAGAAATGAAGTGCTCCAGTTCAGCAC AAACTGCCCAGAATGCAATGCCCCCGCTCAGACCAACATGAAGCTAGTACAAATCCCTCACTTTAAGGAG GTTATCATCATGGCTACCAACTGCGAGAACTGTGGGCATCGGACCAATGAGGTGAAATCTGGAGGAGCAG TAGAACCCTTGGGCACCAGGATCACCCTCCACATCACAGATGCCTCAGATATGACCAGAGACCTCCTCAA GTCTGAGACTTGCAGTGTGGAAATCCCAGAGCTAGAATTTGAACTGGGAATGGCAGTCCTCGGGGGCAAG TTCACCACACTGGAAGGGCTGCTGAAAGACATCCGGGAACTGGTGACCAAAAATCCTTTCACACTGGGCG ACAGTTCCAATCCTGGACAGACGGAGAGACTACAGGAGTTTAGCCAGAAGATGGACCAGATCATCGAAGG TAACATGAAGGCCCACTTTATTATGGATGATCCAGCAGGAAACAGTTACTTGCAGAATGTGTATGCGCCT GAAGATGATCCTGAGATGAAGGTGGAGCGTTACAAGCGCACCTTTGACCAAAATGAGGAGCTAGGGCTCA ATGACATGAAGACAGAGGGCTATGAGGCAGGCCTGGCTCCGCAACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205721 protein sequence
Red=Cloning site Green=Tags(s) MAASGAVEPGPPGAAVAPSPAPAPPPAPDHLFRPISAEDEEQQPTEIESLCMNCYCNGMTRLLLTKIPFF REIIVSSFSCEHCGWNNTEIQSAGRIQDQGVRYTLSVRALEDMNREVVKTDSAATRIPELDFEIPAFSQK GALTTVEGLITRAISGLEQDQPARRANKDATAERIDEFIVKLKELKQVASPFTLIIDDPSGNSFVENPHA PQKDDALVITHYNRTRQQEEMLGLQEEAPAEKPEEEDLRNEVLQFSTNCPECNAPAQTNMKLVQIPHFKE VIIMATNCENCGHRTNEVKSGGAVEPLGTRITLHITDASDMTRDLLKSETCSVEIPELEFELGMAVLGGK FTTLEGLLKDIRELVTKNPFTLGDSSNPGQTERLQEFSQKMDQIIEGNMKAHFIMDDPAGNSYLQNVYAP EDDPEMKVERYKRTFDQNEELGLNDMKTEGYEAGLAPQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003904 |
| ORF Size | 1377 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003904.5 |
| RefSeq Size | 1810 bp |
| RefSeq ORF | 1380 bp |
| Locus ID | 8882 |
| UniProt ID | O75312 |
| Domains | Zpr1 |
| MW | 50.9 kDa |
| Gene Summary | The protein encoded by this gene is found in the cytoplasm of quiescent cells but translocates to the nucleolus in proliferating cells. The encoded protein interacts with survival motor neuron protein (SMN1) to enhance pre-mRNA splicing and to induce neuronal differentiation and axonal growth. Defects in this gene or the SMN1 gene can cause spinal muscular atrophy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ZNF259 promotes breast cancer cells invasion and migration via ERK/GSK3β/snail signaling
,Liu, B;Xing, X;Li, X;Guo, Q;Xu, T;Xu, K;,
Cancer Manag Res
,PubMed ID 30214308
[ZNF259]
|
|
ZNF259 inhibits non-small cell lung cancer cells proliferation and invasion by FAK-AKT signaling
,Shan, Y;Cao, W;Wang, T;Jiang, G;Zhang, Y;Yang, X;,
Cancer Manag Res
,PubMed ID 29276408
[ZNF259]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205721L3 | Lenti ORF clone of Human zinc finger protein 259 (ZNF259), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205721L4 | Lenti ORF clone of Human zinc finger protein 259 (ZNF259), mGFP tagged |
CNY 5,890.00 |
|
| RG205721 | ZNF259 (tGFP-tagged) - Human zinc finger protein 259 (ZNF259) |
CNY 5,256.00 |
|
| SC117673 | ZNF259 (untagged)-Human zinc finger protein 259 (ZNF259) |
CNY 3,656.00 |
|
| SC324610 | ZNF259 (untagged)-Human zinc finger protein 259 (ZNF259) |
CNY 3,656.00 |
