VAM1 (MPP6) (NM_016447) Human Tagged ORF Clone

CAT#: RC205667

MPP6 (Myc-DDK-tagged)-Human membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6) (MPP6)

ORF Plasmid: DDK tGFP



  "NM_016447" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 4,024.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


MPP6 mouse monoclonal antibody, clone OTI3H5 (formerly 3H5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms p55T; PALS2; VAM-1; VAM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205667 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCAAGTCTTGGAAAACCTTACGGAGCTGCCCTCGTCTACTGGAGCAGAAGAAATAGACCTAATTT
TCCTCAAGGGAATTATGGAGAATCCTATTGTAAAATCACTTGCTAAGGCTCATGAGAGGCTAGAAGATTC
CAAACTAGAAGCTGTCAGTGACAATAACTTGGAATTAGTCAATGAAATTCTTGAAGACATCACTCCTCTA
ATAAATGTGGATGAAAATGTGGCAGAATTGGTTGGTATACTCAAAGAACCTCACTTCCAGTCACTGTTGG
AGGCCCATGATATTGTGGCATCAAAGTGTTATGATTCACCTCCATCAAGCCCAGAAATGAATAATTCTTC
TATCAATAATCAGTTATTACCAGTAGATGCCATTCGTATTCTTGGTATTCACAAAAGAGCTGGGGAACCA
CTGGGTGTGACATTTAGGGTTGAAAATAATGATCTGGTAATTGCCCGAATCCTCCATGGGGGAATGATAG
ATCGACAAGGTCTACTTCATGTGGGAGATATAATTAAAGAAGTCAATGGCCATGAGGTTGGAAATAATCC
AAAGGAATTACAAGAATTACTGAAAAATATTAGTGGAAGTGTCACCCTAAAAATCTTACCAAGTTATAGA
GATACCATTACTCCTCAACAGGTATTTGTGAAGTGTCATTTTGATTATAATCCATACAATGACAACCTAA
TACCTTGCAAAGAAGCAGGATTGAAGTTTTCCAAAGGAGAAATTCTTCAGATTGTAAATAGAGAAGATCC
AAATTGGTGGCAGGCTAGCCATGTAAAAGAGGGAGGAAGCGCTGGTCTCATTCCAAGCCAGTTCCTGGAA
GAGAAGAGAAAGGCATTTGTTAGAAGAGACTGGGACAATTCAGGACCTTTTTGTGGAACTATAAGTAGCA
AAAAAAAGAAAAAGATGATGTATCTCACAACCAGAAATGCAGAATTTGATCGTCATGAAATCCAGATATA
TGAGGAGGTAGCCAAAATGCCTCCCTTCCAGAGAAAAACATTAGTATTGATAGGAGCTCAAGGTGTAGGC
CGAAGAAGCTTGAAAAACAGGTTCATAGTATTGAATCCCACTAGATTTGGAACTACGGTGCCATTTACTT
CACGGAAACCAAGGGAAGATGAAAAAGATGGCCAGGCATATAAGTTTGTGTCACGATCTGAGATGGAAGC
AGATATTAAAGCTGGAAAGTATTTGGAACATGGGGAATATGAAGGAAATCTCTATGGAACCAAAATTGAT
TCTATTCTTGAGGTTGTCCAAACTGGACGGACTTGCATTCTGGATGTCAACCCACAAGCACTGAAAGTAT
TGAGGACATCAGAGTTTATGCCCTATGTGGTATTTATTGCGGCTCCGGAGCTAGAGACGTTACGTGCCAT
GCACAAGGCTGTGGTGGATGCAGGAATCACTACCAAGCTTCTGACCGACTCTGACTTGAAGAAAACAGTG
GATGAAAGTGCACGGATTCAGAGAGCATACAACCACTATTTTGATTTGATCATCATAAATGATAATCTAG
ACAAAGCCTTTGAAAAACTGCAAACTGCCATAGAGAAACTGAGAATGGAACCACAGTGGGTCCCAATCAG
CTGGGTTTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205667 protein sequence
Red=Cloning site Green=Tags(s)

MQQVLENLTELPSSTGAEEIDLIFLKGIMENPIVKSLAKAHERLEDSKLEAVSDNNLELVNEILEDITPL
INVDENVAELVGILKEPHFQSLLEAHDIVASKCYDSPPSSPEMNNSSINNQLLPVDAIRILGIHKRAGEP
LGVTFRVENNDLVIARILHGGMIDRQGLLHVGDIIKEVNGHEVGNNPKELQELLKNISGSVTLKILPSYR
DTITPQQVFVKCHFDYNPYNDNLIPCKEAGLKFSKGEILQIVNREDPNWWQASHVKEGGSAGLIPSQFLE
EKRKAFVRRDWDNSGPFCGTISSKKKKKMMYLTTRNAEFDRHEIQIYEEVAKMPPFQRKTLVLIGAQGVG
RRSLKNRFIVLNPTRFGTTVPFTSRKPREDEKDGQAYKFVSRSEMEADIKAGKYLEHGEYEGNLYGTKID
SILEVVQTGRTCILDVNPQALKVLRTSEFMPYVVFIAAPELETLRAMHKAVVDAGITTKLLTDSDLKKTV
DESARIQRAYNHYFDLIIINDNLDKAFEKLQTAIEKLRMEPQWVPISWVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016447
ORF Size 1620 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016447.2, NP_057531.2
RefSeq Size 2201 bp
RefSeq ORF 1623 bp
Locus ID 51678
UniProt ID Q9NZW5
Domains SH3, PDZ, L27, Guanylate_kin, GuKc
Protein Families Druggable Genome, Stem cell - Pluripotency
MW 61.1 kDa
Gene Summary Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily (Tseng et al., 2001 [PubMed 11311936]).[supplied by OMIM, Mar 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...