Choline dehydrogenase (CHDH) (NM_018397) Human Tagged ORF Clone
CAT#: RC205552
CHDH (Myc-DDK-tagged)-Human choline dehydrogenase (CHDH), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_018397" in other vectors (4)
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CNY 4,424.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205552 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTGTCTCCTACGAGGCCTGGGCCGGCCTGGAGCCCTGGCACGGGGAGCCCTGGGGCAGCAGCAAT CCCTGGGTGCCCGGGCCCTGGCCAGCGCAGGCTCTGAGAGCCGGGACGAGTACAGCTATGTGGTGGTGGG CGCGGGCTCGGCGGGCTGCGTGCTGGCTGGGAGGCTCACGGAGGACCCCGCCGAGCGCGTGCTGCTGCTG GAGGCCGGGCCCAAGGACGTGCGCGCGGGGAGCAAGCGGCTCTCGTGGAAGATCCACATGCCCGCGGCCC TGGTGGCCAACCTGTGCGACGACAGGTACAACTGGTGCTACCACACAGAGGTGCAGCGGGGCCTGGACGG CCGCGTGCTGTACTGGCCACGCGGCCGCGTCTGGGGTGGCTCCTCATCCCTCAATGCCATGGTCTACGTC CGTGGGCACGCCGAGGACTACGAGCGCTGGCAGCGCCAGGGCGCCCGCGGCTGGGACTACGCGCACTGCC TGCCCTACTTCCGCAAGGCGCAGGGCCACGAGCTGGGCGCCAGCCGGTACCGGGGCGCCGATGGCCCGCT GCGGGTGTCCCGGGGCAAGACCAACCACCCGCTGCACTGCGCATTCCTGGAGGCCACGCAGCAGGCCGGC TACCCGCTCACCGAGGACATGAATGGCTTCCAGCAGGAGGGCTTCGGCTGGATGGACATGACCATCCATG AAGGCAAACGGTGGAGCGCAGCCTGTGCCTACCTGCACCCAGCACTGAGCCGCACCAACCTCAAGGCCGA GGCCGAGACGCTTGTGAGCAGGGTGCTATTTGAGGGCACCCGTGCAGTGGGCGTGGAGTATGTCAAGAAT GGCCAGAGCCACAGGGCTTATGCCAGCAAGGAGGTGATTCTGAGTGGAGGTGCCATCAACTCTCCACAGC TGCTCATGCTCTCTGGCATCGGGAATGCTGATGACCTCAAGAAACTGGGCATCCCTGTGGTGTGCCACCT ACCTGGGGTTGGCCAGAACCTGCAAGACCACCTGGAGATCTACATTCAGCAGGCATGCACCCGCCCTATC ACCCTCCATTCAGCACAGAAGCCCCTGCGGAAGGTCTGCATTGGTCTGGAGTGGCTCTGGAAATTCACAG GGGAGGGAGCCACTGCCCATCTGGAAACAGGTGGGTTCATCCGCAGCCAGCCTGGGGTCCCCCACCCGGA CATCCAGTTCCATTTCCTGCCATCCCAAGTGATTGACCACGGGCGGGTCCCCACCCAGCAGGAGGCTTAC CAGGTACATGTGGGGCCCATGCGGGGCACGAGTGTGGGCTGGCTCAAACTGAGAAGTGCCAATCCCCAAG ACCACCCTGTGATCCAGCCCAACTACTTGTCAACAGAAACTGATATTGAGGATTTCCGTCTGTGTGTGAA GCTCACCAGAGAAATTTTTGCACAGGAAGCCCTGGCTCCGTTCCGAGGGAAAGAGCTCCAGCCAGGAAGC CACATTCAGTCAGATAAAGAGATAGATGCCTTTGTGCGGGCAAAAGCCGACAGCGCCTACCACCCCTCGT GCACCTGTAAGATGGGCCAGCCCTCCGATCCCACTGCCGTGGTGGATCCGCAGACAAGGGTCCTCGGGGT GGAAAACCTCAGGGTCGTCGATGCCTCCATCATGCCTAGCATGGTCAGCGGCAACCTGAACGCCCCCACA ATCATGATCGCAGAGAAGGCAGCTGACATTATCAAGGGGCAGCCTGCACTCTGGGACAAAGATGTCCCTG TCTACAAGCCCAGGACGCTGGCCACCCAGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205552 protein sequence
Red=Cloning site Green=Tags(s) MWCLLRGLGRPGALARGALGQQQSLGARALASAGSESRDEYSYVVVGAGSAGCVLAGRLTEDPAERVLLL EAGPKDVRAGSKRLSWKIHMPAALVANLCDDRYNWCYHTEVQRGLDGRVLYWPRGRVWGGSSSLNAMVYV RGHAEDYERWQRQGARGWDYAHCLPYFRKAQGHELGASRYRGADGPLRVSRGKTNHPLHCAFLEATQQAG YPLTEDMNGFQQEGFGWMDMTIHEGKRWSAACAYLHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKN GQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPI TLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAY QVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGS HIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPT IMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018397 |
ORF Size | 1782 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018397.2 |
RefSeq Size | 3711 bp |
RefSeq ORF | 1785 bp |
Locus ID | 55349 |
UniProt ID | Q8NE62 |
Protein Pathways | Glycine, serine and threonine metabolism |
MW | 65.4 kDa |
Gene Summary | The protein encoded by this gene is a choline dehydrogenase that localizes to the mitochondrion. Variations in this gene can affect susceptibility to choline deficiency. A few transcript variants have been found for this gene, but the full-length nature of only one has been characterized to date. [provided by RefSeq, Dec 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205552L3 | Lenti ORF clone of Human choline dehydrogenase (CHDH), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205552L4 | Lenti ORF clone of Human choline dehydrogenase (CHDH), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RG205552 | CHDH (tGFP-tagged) - Human choline dehydrogenase (CHDH), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
SC304586 | CHDH (untagged)-Human choline dehydrogenase (CHDH), nuclear gene encoding mitochondrial protein |
CNY 7,220.00 |
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