SLC25A31 (NM_031291) Human Tagged ORF Clone

CAT#: RC205546

SLC25A31 (Myc-DDK-tagged)-Human solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 31 (SLC25A31), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_031291" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal anti-SLC25A31 antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AAC4; ANT 4; ANT4; SFEC35kDa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205546 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCGTGAGCCTGCGAAAAAGAAGGCAGAAAAGCGGCTGTTTGACGCCTCATCCTTCGGGAAGGACC
TTCTGGCCGGCGGAGTCGCGGCAGCTGTGTCCAAGACAGCGGTGGCGCCCATCGAGCGGGTGAAGCTGCT
GCTGCAGGTGCAGGCGTCGTCGAAGCAGATCAGCCCCGAGGCGCGGTACAAAGGCATGGTGGACTGCCTG
GTGCGGATTCCTCGCGAGCAGGGTTTCTTCAGTTTTTGGCGTGGCAATTTGGCAAATGTTATTCGGTATT
TTCCAACACAAGCTCTAAACTTTGCTTTTAAGGACAAATACAAGCAGCTATTCATGTCTGGAGTTAATAA
AGAAAAACAGTTCTGGAGGTGGTTTTTGGCAAACCTGGCTTCTGGTGGAGCTGCTGGGGCAACATCCTTA
TGTGTAGTATATCCTCTAGATTTTGCCCGAACCCGATTAGGTGTCGATATTGGAAAAGGTCCTGAGGAGC
GACAATTCAAGGGTTTAGGTGACTGTATTATGAAAATAGCAAAATCAGATGGAATTGCTGGTTTATACCA
AGGGTTTGGTGTTTCAGTACAGGGCATCATTGTGTACCGAGCCTCTTATTTTGGAGCTTATGACACAGTT
AAGGGTTTATTACCAAAGCCAAAGAAAACTCCATTTCTTGTCTCCTTTTTCATTGCTCAAGTTGTGACTA
CATGCTCTGGAATACTTTCTTATCCCTTTGACACAGTTAGAAGACGTATGATGATGCAGAGTGGTGAGGC
TAAACGGCAATATAAAGGAACCTTAGACTGCTTTGTGAAGATATACCAACATGAAGGAATCAGTTCCTTT
TTTCGTGGCGCCTTCTCCAATGTTCTTCGCGGTACAGGGGGTGCTTTGGTGTTGGTATTATATGATAAAA
TTAAAGAATTCTTTCATATTGATATTGGTGGTAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205546 protein sequence
Red=Cloning site Green=Tags(s)

MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCL
VRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAGATSL
CVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGIIVYRASYFGAYDTV
KGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSF
FRGAFSNVLRGTGGALVLVLYDKIKEFFHIDIGGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031291
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031291.4
RefSeq Size 1821 bp
RefSeq ORF 948 bp
Locus ID 83447
UniProt ID Q9H0C2
Protein Families Druggable Genome, Transmembrane
Protein Pathways Calcium signaling pathway, Huntington's disease, Parkinson's disease
MW 35 kDa
Gene Summary The protein encoded by this gene is a member of the ADP/ATP carrier family of proteins that exchange cytosolic ADP for matrix ATP in the mitochondria. Cells over-expressing this gene have been shown to display an anti-apoptotic phenotype. This protein is also thought to play a role in spermatogenesis, where it is believed to associate with a part of the flagellar cytoskeleton and with glycolytic enzymes. Male mice with mutations in the mouse ortholog of this gene are sterile and spermatocytes display an early meiotic arrest phenotype. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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