RBJ (DNAJC27) (NM_016544) Human Tagged ORF Clone
CAT#: RC205500
DNAJC27 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1
ORF Plasmid: tGFP
"NM_016544" in other vectors (6)
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CNY 3,600.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RabJS; RBJ |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205500 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTGGCAGGTCTCTCCGCATCAAAGTCATCTCCATGGGCA ACGCCGAAGTGGGGAAAAGCTGTATTATAAAGCGATACTGTGAGAAAAGATTCGTGTCTAAATACCTGGC AACAATTGGAATTGACTATGGAGTCACAAAGGTACACGTCAGAGACAGAGAAATCAAAGTTAACATCTTT GATATGGCTGGACATCCCTTCTTCTATGAGGTTCGAAATGAGTTTTACAAGGACACACAGGGTGTGATAC TGGTCTATGATGTTGGGCAGAAAGACTCCTTTGACGCCCTTGATGCGTGGCTGGCAGAAATGAAGCAAGA GCTTGGACCTCATGGAAACATGGAAAATATTATATTTGTAGTTTGTGCCAACAAGATTGATTGTACCAAA CATCGCTGTGTAGATGAAAGTGAAGGACGTCTTTGGGCTGAAAGCAAAGGGTTCCTGTACTTTGAAACTT CAGCACAAACTGGAGAAGGCATTAATGAGATGTTCCAGACCTTTTATATATCCATAGTTGATTTATGTGA AAATGGCGGGAAACGCCCTACCACCAATAGCAGTGCTAGTTTCACCAAAGAACAAGCAGATGCCATTCGC AGAATTCGAAATAGTAAAGACAGTTGGGACATGCTGGGAGTCAAACCTGGGGCCTCAAGGGATGAAGTCA ATAAAGCGTATCGGAAACTTGCTGTGCTTCTTCACCCTGACAAATGTGTAGCACCTGGCAGTGAAGATGC CTTCAAAGCAGTTGTGAATGCTCGGACAGCCCTCCTGAAAAACATCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205500 protein sequence
Red=Cloning site Green=Tags(s) MEANMPKRKEPGRSLRIKVISMGNAEVGKSCIIKRYCEKRFVSKYLATIGIDYGVTKVHVRDREIKVNIF DMAGHPFFYEVRNEFYKDTQGVILVYDVGQKDSFDALDAWLAEMKQELGPHGNMENIIFVVCANKIDCTK HRCVDESEGRLWAESKGFLYFETSAQTGEGINEMFQTFYISIVDLCENGGKRPTTNSSASFTKEQADAIR RIRNSKDSWDMLGVKPGASRDEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLKNIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016544 |
ORF Size | 819 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016544.1, NP_057628.1 |
RefSeq Size | 5008 bp |
RefSeq ORF | 822 bp |
Locus ID | 51277 |
UniProt ID | Q9NZQ0 |
Domains | DnaJ, ras, RAN, RAS, RHO, RAB |
MW | 30.9 kDa |
Gene Summary | GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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MC4R Variant rs17782313 Associates With Increased Levels of DNAJC27, Ghrelin, and Visfatin and Correlates With Obesity and Hypertension in a Kuwaiti Cohort
,Hammad, MM;Abu-Farha, M;Hebbar, P;Cherian, P;Al Khairi, I;Melhem, M;Alkayal, F;Alsmadi, O;Thanaraj, TA;Al-Mulla, F;Abubaker, J;,
Front Endocrinol (Lausanne) 2020
,PubMed ID 32733386
[RBJ]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205500L1 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
RC205500L2 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC205500L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205500L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG205500 | DNAJC27 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1 |
CNY 5,200.00 |
|
SC100369 | DNAJC27 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1 |
CNY 3,600.00 |