ATP6V1B2 (NM_001693) Human Tagged ORF Clone
CAT#: RC205447
ATP6V1B2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)
ORF Plasmid: tGFP
"NM_001693" in other vectors (6)
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CNY 3,808.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ATP6B1B2; ATP6B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205447 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCACCGGTGGGC CGGCGGTGGGAGCTCAGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTCCCAGCCTCGCCTCACATA CAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGATCATGTTAAGTTTCCCAGGTATGCTGAA ATTGTCCATTTGACCTTACCGGATGGCACAAAGAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGG CAGTAGTTCAGGTATTTGAAGGGACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGA TATTCTCCGAACACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGAC AGAGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCAATGTCGAA TCTACCCAGAGGAAATGATTCGGACTGGCATTTCGGCCATCGATGGGATGAACAGTATTGCTAGGGGGCA GAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGAGATTGCAGCTCAGATCTGTCGCCAGGCT GGTTTGGTAAAGAAATCCAAAGATGTAGTAGACTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTA TGGGTGTAAACATGGAAACTGCCCGGTTCTTCAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT CTGCCTCTTTTTGAACTTGGCTAATGACCCAACCATTGAGCGAATTATCACTCCTCGCCTGGCTCTAACC ACAGCTGAATTTCTGGCGTACCAATGTGAGAAACATGTATTGGTTATTCTAACAGACATGAGTTCTTATG CTGAAGCACTTCGAGAGGTTTCAGCAGCCAGGGAAGAGGTACCTGGTCGACGAGGTTTTCCAGGTTACAT GTATACAGATTTAGCCACGATATATGAACGCGCTGGGCGAGTGGGAGGGAGAAACGGCTCGATTACTCAA ATCCCTATTCTAACCATGCCTAATGATGATATCACTCACCCCATCCCAGACTTGACTGGCTACATTACAG AGGGGCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTATCCACCTATCAATGTGCTGCCCTC ACTATCACGGTTAATGAAGTCTGCTATTGGAGAAGGGATGACCAGGAAGGATCATGCCGATGTATCTAAC CAGCTATATGCGTGCTATGCTATTGGAAAGGATGTGCAAGCCATGAAAGCTGTCGTTGGAGAAGAAGCCC TTACCTCAGATGATCTTCTCTACTTGGAATTTCTGCAGAAGTTTGAGAGGAACTTCATTGCTCAGGGTCC TTACGAAAATCGCACTGTCTTTGAGACTTTGGACATTGGCTGGCAGCTACTCCGAATCTTCCCCAAAGAA ATGCTGAAGAGAATCCCTCAGAGCACCCTCAGCGAATTTTACCCTCGAGACTCTGCAAAGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205447 protein sequence
Red=Cloning site Green=Tags(s) MALRAMRGIVNGAAPELPVPTGGPAVGAQEQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAE IVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPID RGPVVLAEDFLDIMGQPINPQCRIYPEEMIRTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQA GLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALT TAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVGGRNGSITQ IPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSN QLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKE MLKRIPQSTLSEFYPRDSAKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001693 |
ORF Size | 1533 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 3054 bp |
RefSeq ORF | 1536 bp |
Locus ID | 526 |
UniProt ID | P21281 |
Domains | ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N |
Protein Families | Druggable Genome |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
MW | 56.4 kDa |
Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205447L1 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), Myc-DDK-tagged |
CNY 6,208.00 |
|
RC205447L2 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), mGFP tagged |
CNY 5,890.00 |
|
RC205447L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205447L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2), mGFP tagged |
CNY 5,890.00 |
|
RG205447 | ATP6V1B2 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2) |
CNY 5,408.00 |
|
SC119083 | ATP6V1B2 (untagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2) |
CNY 7,088.00 |
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