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GABA A Receptor alpha 1 (GABRA1) (NM_000806) Human Tagged ORF Clone
CAT＃: RC205390
GABRA1 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1
ORF Plasmid: tGFP
"NM_000806" in other vectors (6)
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CNY 5,488.00
| Cited in 8 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DEE19; ECA4; EIEE19; EJM; EJM5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205390 representing NM_000806
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAAAAGTCCAGGTCTGTCTGACTGTCTTTGGGCCTGGATCCTCCTTCTGAGCACACTGACTGGAA GAAGCTATGGACAGCCGTCATTACAAGATGAACTTAAAGACAATACCACTGTCTTCACCAGGATTTTGGA CAGACTCCTAGATGGTTATGACAATCGCCTGAGACCAGGATTGGGAGAGCGTGTAACCGAAGTGAAGACT GATATCTTCGTCACCAGTTTCGGACCCGTTTCAGACCATGATATGGAATATACAATAGATGTATTTTTCC GTCAAAGCTGGAAGGATGAAAGGTTAAAATTTAAAGGACCTATGACAGTCCTCCGGTTAAATAACCTAAT GGCAAGTAAAATCTGGACTCCGGACACATTTTTCCACAATGGAAAGAAGTCAGTGGCCCACAACATGACC ATGCCCAACAAACTCCTGCGGATCACAGAGGATGGCACCTTGCTGTACACCATGAGGCTGACAGTGAGAG CTGAATGTCCGATGCATTTGGAGGACTTCCCTATGGATGCCCATGCTTGCCCACTAAAATTTGGAAGTTA TGCTTATACAAGAGCAGAAGTTGTTTATGAATGGACCAGAGAGCCAGCACGCTCAGTGGTTGTAGCAGAA GATGGATCACGTCTAAACCAGTATGACCTTCTTGGACAAACAGTAGACTCTGGAATTGTCCAGTCAAGTA CAGGAGAATATGTTGTTATGACCACTCATTTCCACTTGAAGAGAAAGATTGGCTACTTTGTTATTCAAAC ATACCTGCCATGCATAATGACAGTGATTCTCTCACAAGTCTCCTTCTGGCTCAACAGAGAGTCTGTACCA GCAAGAACTGTCTTTGGAGTAACAACTGTGCTCACCATGACAACATTGAGCATCAGTGCCAGAAACTCCC TCCCTAAGGTGGCTTATGCAACAGCTATGGATTGGTTTATTGCCGTGTGCTATGCCTTTGTGTTCTCAGC TCTGATTGAGTTTGCCACAGTAAACTATTTCACTAAGAGAGGTTATGCATGGGATGGCAAAAGTGTGGTT CCAGAAAAGCCAAAGAAAGTAAAGGATCCTCTTATTAAGAAAAACAACACTTACGCTCCAACAGCAACCA GCTACACCCCTAATTTGGCCAGGGGCGACCCGGGCTTAGCCACCATTGCTAAAAGTGCAACCATAGAACC TAAAGAGGTCAAGCCCGAAACAAAACCACCAGAACCCAAGAAAACCTTTAACAGTGTCAGCAAAATTGAC CGACTGTCAAGAATAGCCTTCCCGCTGCTATTTGGAATCTTTAACTTAGTCTACTGGGCTACGTATTTAA ACAGAGAGCCTCAGCTAAAAGCCCCCACACCACATCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205390 representing NM_000806
Red=Cloning site Green=Tags(s) MRKSPGLSDCLWAWILLLSTLTGRSYGQPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKT DIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMT MPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAE DGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP ARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVV PEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKID RLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000806 |
| ORF Size | 1368 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000806.5 |
| RefSeq Size | 3678 bp |
| RefSeq ORF | 1371 bp |
| Locus ID | 2554 |
| UniProt ID | P14867 |
| Domains | Neur_chan_memb, Neur_chan_LBD |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 51.6 kDa |
| Gene Summary | This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene cause juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Multiple transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008] |
Citations (8)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Quantitative interactome proteomics identifies a proteostasis network for GABA A receptors
,null,
The Journal of Biological Chemistry
,PubMed ID 36030824
[GABA A Receptor alpha 1]
|
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Proteostasis Regulators Restore Function of Epilepsy-Associated GABAA Receptors
,Di, XJ;Wang, YJ;Cotter, E;Wang, M;Whittsette, AL;Han, DY;Sangwung, P;Brown, R;Lynch, JW;Keramidas, A;Mu, TW;,
Cell Chem Biol
,PubMed ID 32888501
[GABA A Receptor alpha 1]
|
|
Preclinical characterization of zuranolone (SAGE-217), a selective neuroactive steroid GABAA receptor positive allosteric modulator
,Althaus, AL;Ackley, MA;Belfort, GM;Gee, SM;Dai, J;Nguyen, DP;Kazdoba, TM;Modgil, A;Davies, PA;Moss, SJ;Salituro, FG;Hoffmann, E;Hammond, RS;Robichaud, AJ;Quirk, MC;Doherty, JJ;,
Neuropharmacology
,PubMed ID 32976892
[GABA A Receptor alpha 1]
|
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LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors
,Fu, YL;Zhang, B;Mu, TW;,
Biochem. Biophys. Res. Commun.
,PubMed ID 30791981
[GABA A Receptor alpha 1]
|
|
Grp94 Delivers ?-aminobutyric Acid Type A (GABAA) Receptors to Hrd1-Mediatd Endoplasmic Reticulum-Associated Degradation
,Di, XJ;Wang, YJ;Han, DY;Fu, YL;Duerfeldt, AS;Blagg, BS;Mu, TW;,
J. Biol. Chem.
,PubMed ID 26945068
[GABA A Receptor alpha 1]
|
|
Structure-dependent inhibition of the human a 1 ß 2 ? 2 GABA A receptor by piperazine derivatives: A novel mode of action
,Hondebrink, L;Hermans, EJP;Schmeink, S;,
Neurotoxicology
,PubMed ID 26344803
[GABA A Receptor alpha 1]
|
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L-type Calcium Channel Blockers Enhance Trafficking and Function of Epilepsy-associated a1(D219N) Subunits of GABAA Receptors
,Han, DY;Guan, BJ;Wang, YJ;Hatzoglou, M;Mu, TW;,
ACS Chem. Biol.
,PubMed ID 26168288
[GABA A Receptor alpha 1]
|
|
Combining VCP Inhibition and SAHA Treatment Additively Enhances the Folding, Trafficking, and Function of Epilepsy-Associated GABAA Receptors
,Han, DY;Di, XJ;Fu, YL;Mu, TW;,
J. Biol. Chem.
,PubMed ID 25406314
[GABA A Receptor alpha 1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205390L1 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205390L2 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205390L3 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC205390L4 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG205390 | GABRA1 (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1 |
CNY 7,088.00 |
|
| SC119668 | GABRA1 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1), transcript variant 1 |
CNY 5,488.00 |
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