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TRIM23 (NM_001656) Human Tagged ORF Clone
CAT＃: RC205383
- TrueORF®
TRIM23 (Myc-DDK-tagged)-Human tripartite motif containing 23 (TRIM23), transcript variant alpha
ORF Plasmid: tGFP
"NM_001656" in other vectors (6)
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CNY 4,272.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARD1; ARFD1; RNF46 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205383 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACCCTGGTTGTAAACAAGCTCGGAGCGGGAGTAGACAGTGGCCGGCAGGGCAGCCGGGGGACAG CTGTAGTGAAGGTGCTAGAGTGTGGAGTTTGTGAAGATGTCTTTTCTTTGCAAGGAGACAAAGTTCCCCG TCTTTTGCTTTGTGGCCATACCGTCTGTCATGACTGTCTCACTCGCCTACCTCTTCATGGAAGAGCAATC CGTTGCCCATTTGATCGACAAGTAACAGACCTAGGTGATTCAGGTGTCTGGGGATTGAAAAAAAATTTTG CTTTATTGGAGCTTTTGGAACGACTGCAGAATGGGCCTATTGGTCAGTATGGAGCTGCAGAAGAATCCAT TGGGATATCTGGAGAGAGCATCATTCGTTGTGATGAAGATGAAGCTCACCTTGCCTCTGTATATTGCACT GTGTGTGCAACTCATTTGTGCTCTGAGTGTTCTCAAGTTACTCATTCTACAAAGACATTAGCAAAGCACA GGCGAGTTCCTCTAGCTGATAAACCTCATGAGAAAACTATGTGCTCTCAGCACCAGGTGCATGCCATTGA GTTTGTTTGCTTGGAAGAAGGTTGTCAAACTAGCCCACTCATGTGCTGTGTCTGCAAAGAATATGGAAAA CACCAGGGTCACAAGCATTCAGTATTGGAACCAGAAGCTAATCAGATCCGAGCATCAATTTTAGATATGG CTCACTGCATACGGACCTTCACAGAGGAAATCTCAGATTATTCCAGAAAATTAGTTGGAATTGTGCAGCA CATTGAAGGAGGAGAACAAATCGTGGAAGATGGAATTGGAATGGCTCACACAGAACATGTACCAGGGACT GCAGAGAATGCCCGGTCATGTATTCGAGCTTATTTTTATGATCTACATGAAACTCTGTGTCGTCAAGAAG AAATGGCTCTAAGTGTTGTTGATGCTCATGTTCGTGAAAAATTGATTTGGCTCAGGCAGCAACAAGAAGA TATGACTATTTTGTTGTCAGAGGTTTCTGCAGCCTGCCTCCACTGTGAAAAGACTTTGCAGCAGGATGAT TGTAGAGTTGTCTTGGCAAAACAGGAAATTACAAGGTTACTGGAAACATTGCAGAAACAGCAGCAGCAGT TTACAGAAGTTGCAGATCACATTCAGTTGGATGCCAGCATCCCTGTCACTTTTACAAAGGATAATCGAGT TCACATTGGACCAAAAATGGAAATTCGGGTCGTTACGTTAGGATTGGATGGTGCTGGAAAAACTACTATC TTGTTTAAGTTAAAACAGGATGAATTCATGCAGCCCATTCCAACAATTGGTTTTAACGTGGAAACTGTAG AATATAAAAATCTAAAATTCACTATTTGGGATGTAGGTGGAAAACACAAATTAAGACCATTGTGGAAACA TTATTACCTCAATACTCAAGCTGTTGTGTTTGTTGTAGATAGCAGTCATAGAGACAGAATTAGTGAAGCA CACAGCGAACTTGCAAAGTTGTTAACGGAAAAAGAACTCCGAGATGCTCTGCTCCTGATTTTTGCTAACA AACAGGATGTTGCTGGAGCACTGTCAGTAGAAGAAATCACTGAACTACTCAGTCTCCATAAATTATGCTG TGGCCGTAGCTGGTATATTCAGGGCTGTGATGCTCGAAGTGGTATGGGACTGTATGAAGGGTTGGACTGG CTCTCACGGCAACTTGTAGCTGCTGGAGTATTGGATGTTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205383 protein sequence
Red=Cloning site Green=Tags(s) MATLVVNKLGAGVDSGRQGSRGTAVVKVLECGVCEDVFSLQGDKVPRLLLCGHTVCHDCLTRLPLHGRAI RCPFDRQVTDLGDSGVWGLKKNFALLELLERLQNGPIGQYGAAEESIGISGESIIRCDEDEAHLASVYCT VCATHLCSECSQVTHSTKTLAKHRRVPLADKPHEKTMCSQHQVHAIEFVCLEEGCQTSPLMCCVCKEYGK HQGHKHSVLEPEANQIRASILDMAHCIRTFTEEISDYSRKLVGIVQHIEGGEQIVEDGIGMAHTEHVPGT AENARSCIRAYFYDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSEVSAACLHCEKTLQQDD CRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTI LFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEA HSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDW LSRQLVAAGVLDVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001656 |
| ORF Size | 1722 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001656.4 |
| RefSeq Size | 3914 bp |
| RefSeq ORF | 1725 bp |
| Locus ID | 373 |
| UniProt ID | P36406 |
| Domains | zf-B_box, RING, RAB, BBC, SAR, ARF, arf |
| Protein Families | Druggable Genome |
| MW | 64.1 kDa |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205383L1 | Lenti ORF clone of Human tripartite motif containing 23 (TRIM23), transcript variant alpha, Myc-DDK-tagged |
CNY 6,672.00 |
|
| RC205383L2 | Lenti ORF clone of Human tripartite motif containing 23 (TRIM23), transcript variant alpha, mGFP tagged |
CNY 6,460.00 |
|
| RC205383L3 | Lenti ORF clone of Human tripartite motif containing 23 (TRIM23), transcript variant alpha, Myc-DDK-tagged |
CNY 6,672.00 |
|
| RC205383L4 | Lenti ORF clone of Human tripartite motif containing 23 (TRIM23), transcript variant alpha, mGFP tagged |
CNY 6,460.00 |
|
| RG205383 | TRIM23 (tGFP-tagged) - Human tripartite motif containing 23 (TRIM23), transcript variant alpha |
CNY 5,872.00 |
|
| SC119129 | TRIM23 (untagged)-Human tripartite motif containing 23 (TRIM23), transcript variant alpha |
CNY 4,280.00 |
