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SFRP4 (NM_003014) Human Tagged ORF Clone
CAT＃: RC205365
SFRP4 (Myc-DDK-tagged)-Human secreted frizzled-related protein 4 (SFRP4)
ORF Plasmid: tGFP
"NM_003014" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FRP-4; FRPHE; FRZB-2; PYL; sFRP-4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205365 representing NM_003014
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGGCTGGCTGCGCTCCGAGCTGCGGAGTCCGGGACTGGAGCTGCCCGGGCGGGTTCGCGCCCC GAAGGCTGAGAGCTGGCGCTGCTCGTGCCCTGTGTGCCAGACGGCGGAGCTCCGCGGCCGGACCCCGCGG CCCCGTTTTGCTGCCGACTGGAGTTTGGGGGAAGAAACTCTCCTGCGCCCCAGAGGATTTCTTCCTCGGC GAAGGGACAGCGAAAGATGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTGTCTGCCGGGGTCGCAGCGCGA GAGGGCAGTGCCATGTTCCTCTCCATCCTAGTGGCGCTGTGCCTGTGGCTGCACCTGGCGCTGGCGTGCG CGGCGCGCCCTGCGAGGCGGTGCGCATCCCTATGTGCCGGCACATGCCCTGGAACATCACGCGGATGCCC AACCACCTGCACCACAGCACGCAGGAGAACGCCATCCTGGCCATCGAGCAGTACGAGGAGCTGGTGGACG TGAACTGCAGCGCCGTGCTGCGCTTCTTCCTCTGTGCCATGTACGCGCCCATTTGCACCCTGGAGTTCCT GCACGACCCTATCAAGCCGTGCAAGTCGGTGTGCCAACGCGCGCGCGACGACTGCGAGCCCCTCATGAAG ATGTACAACCACAGCTGGCCCGAAAGCCTGGCCTGCGACGAGCTGCCTGTCTATGACCGTGGCGTGTGCA TCTCGCCTGAAGCCATCGTCACGGACCTCCCGGAGGATGTTAAGTGGATAGACATCACACCAGACATGAT GGTACAGGAAAGGCCTCTTGATGTTGACTGTAAACGCCTAAGCCCCGATCGGTGCAAGTGTAAAAAGGTG AAGCCAACTTTGGCAACGTATCTCAGCAAAAACTACAGCTATGTTATTCATGCCAAAATAAAAGCTGTGC AGAGGAGTGGCTGCAATGAGGTCACAACGGTGGTGGATGTAAAAGAGATCTTCAAGTCCTCATCACCCAT CCCTCGAACTCAAGTCCCGCTCATTACAAATTCTTCTTGCCAGTGTCCACACATCCTGCCCCATCAAGAT GTTCTCATCATGTGTTACGAGTGGCGTTCAAGGATGATGCTTCTTGAAAATTGCTTAGTTGAAAAATGGA GAGATCAGCTTAGTAAAAGATCCATACAGTGGGAAGAGAGGCTGCAGGAACAGCGGAGAACAGTTCAGGA CAAGAAGAAAACAGCCGGGCGCACCAGTCGTAGTAATCCCCCCAAACCAAAGGGAAAGCCTCCTGCTCCC AAACCAGCCAGTCCCAAGAAGAACATTAAAACTAGGAGTGCCCGGAAGAGAACAAACCCGAAAAGAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205365 representing NM_003014
Red=Cloning site Green=Tags(s) MAVAGCAPSCGVRDWSCPGGFAPRRLRAGAARALCARRRSSAAGPRGPVLLPTGVWGKKLSCAPEDFFLG EGTAKDEGGRKRRALSVCRGRSARGQCHVPLHPSGAVPVAAPGAGVRGAPCEAVRIPMCRHMPWNITRMP NHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMK MYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKV KPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQD VLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAP KPASPKKNIKTRSARKRTNPKRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003014 |
| ORF Size | 1329 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 2820 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 6424 |
| UniProt ID | Q6FHJ7 |
| Domains | FRI, NTR |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway |
| Protein Pathways | Wnt signaling pathway |
| MW | 49.56 kDa |
| Gene Summary | Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells
,Corbett, L;Mann, J;Mann, DA;,
PLoS ONE
,PubMed ID 26566235
[SFRP4]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205365L1 | Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205365L2 | Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), mGFP tagged |
CNY 5,890.00 |
|
| RC205365L3 | Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC205365L4 | Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), mGFP tagged |
CNY 6,056.00 |
|
| RG205365 | SFRP4 (tGFP-tagged) - Human secreted frizzled-related protein 4 (SFRP4) |
CNY 5,256.00 |
|
| SC118247 | SFRP4 (untagged)-Human secreted frizzled-related protein 4 (SFRP4) |
CNY 3,656.00 |
