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HSPA1L (NM_005527) Human Tagged ORF Clone
CAT＃: RC205351
HSPA1L (Myc-DDK-tagged)-Human heat shock 70kDa protein 1-like (HSPA1L)
ORF Plasmid: tGFP
"NM_005527" in other vectors (6)
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CNY 7,160.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSP70-1L; HSP70-HOM; HSP70T; hum70t |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205351 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTGCCAAGGGAATCGCCATAGGCATCGACCTGGGCACCACCTACTCCTGTGTGGGGGTGTTCC AGCACGGCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACCACCCCCAGCTACGTGGCCTTCAC AGACACCGAGCGGCTCATTGGGGATGCGGCCAAGAACCAGGTAGCAATGAATCCCCAGAACACTGTTTTT GATGCTAAACGTCTGATCGGCAGGAAATTTAATGATCCTGTTGTACAAGCAGATATGAAACTTTGGCCTT TTCAAGTGATTAATGAAGGAGGCAAGCCCAAAGTCCTTGTGTCCTACAAAGGGGAGAATAAAGCTTTCTA CCCTGAGGAAATCTCTTCGATGGTATTGACTAAGTTGAAGGAGACTGCTGAGGCCTTTTTGGGCCACCCT GTCACCAATGCAGTGATTACCGTGCCAGCCTATTTCAATGACTCTCAACGTCAGGCTACTAAGGATGCAG GTGTGATTGCTGGACTTAATGTGCTAAGAATCATCAATGAGCCCACGGCTGCTGCCATTGCCTATGGTTT AGATAAAGGAGGTCAAGGAGAACGACATGTCCTGATTTTTGATCTGGGTGGAGGCACATTTGATGTGTCA ATTCTGACCATAGATGATGGGATTTTTGAGGTAAAGGCCACTGCTGGGGACACTCACCTGGGTGGGGAGG ACTTTGACAACAGGCTTGTGAGCCACTTCGTGGAGGAGTTCAAGAGGAAACACAAAAAGGACATCAGCCA GAACAAGCGAGCCGTGAGGCGGCTGCGCACCGCCTGCGAGAGGGCCAAGAGGACCCTGTCGTCCAGCACC CAGGCCAACCTAGAAATTGATTCACTTTATGAAGGCATTGACTTCTATACATCCATCACCAGAGCTCGAT TTGAAGAGTTGTGTGCAGACCTGTTTAGGGGTACCCTGGAGCCTGTAGAAAAAGCGCTTCGGGATGCCAA GATGGATAAGGCTAAAATCCATGACATTGTTTTAGTAGGGGGCTCCACCCGCATCCCCAAGGTGCAGCGG CTGCTTCAGGACTACTTCAATGGACGTGATCTCAACAAGAGCATCAACCCTGATGAGGCCGTAGCATATG GGGCTGCGGTACAAGCAGCCATCCTGATGGGGGACAAGTCTGAGAAGGTACAGGACCTGCTGCTGCTGGA CGTGGCTCCCCTGTCCCTGGGGCTGGAGACGGCTGGGGGCGTGATGACTGCCTTGATGAAGCGCAACTCC ACCATCCCCACCAAGCAGACACAGATTTTCACCACCTACTCTGACAACCAACCCGGGGTGCTGATCCAGG TGTATGAGGGCGAGAGGGCCATGACAAAGGACAACAACCTGCTGGGGCGGTTTGACCTGACTGGAATCCC TCCAGCACCCAGGGGAGTTCCTCAGATCGAGGTGACGTTTGACATTGATGCCAATGGTATTCTCAATGTC ACAGCCATGGACAAGAGCACCGGCAAGGTGAACAAGATCACCATCGCCAATGACAAGGGCCGCCTGAGCA AGGAGGAGATTGAGCGCATGGTTCTGGATGCTGAGAAATATAAAGCTGAAGATGAGGTCCAGAGGGAGAA AATTGCTGCAAAGAATGCCTTAGAATCCTATGCTTTTAACATGAAGAGTGTTGTGAGTGATGAAGGTTTG AAGGGCAAGATTAGTGAGTCTGATAAAAATAAAATATTGGATAAATGCAACGAGCTCCTTTCGTGGCTGG AGGTCAATCAACTGGCAGAGAAAGATGAGTTTGATCATAAGAGAAAGGAATTGGAGCAGATGTGTAACCC TATCATCACAAAACTCTACCAAGGAGGATGCACTGGGCCTGCCTGCGGAACAGGGTATGTGCCTGGAAGG CCTGCCACAGGCCCCACAATTGAAGAAGTAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205351 protein sequence
Red=Cloning site Green=Tags(s) MATAKGIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVF DAKRLIGRKFNDPVVQADMKLWPFQVINEGGKPKVLVSYKGENKAFYPEEISSMVLTKLKETAEAFLGHP VTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGGQGERHVLIFDLGGGTFDVS ILTIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSST QANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQR LLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALMKRNS TIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNV TAMDKSTGKVNKITIANDKGRLSKEEIERMVLDAEKYKAEDEVQREKIAAKNALESYAFNMKSVVSDEGL KGKISESDKNKILDKCNELLSWLEVNQLAEKDEFDHKRKELEQMCNPIITKLYQGGCTGPACGTGYVPGR PATGPTIEEVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005527 |
| ORF Size | 1923 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005527.4 |
| RefSeq Size | 2550 bp |
| RefSeq ORF | 1926 bp |
| Locus ID | 3305 |
| UniProt ID | P34931 |
| Domains | HSP70 |
| Protein Pathways | Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome |
| MW | 70.4 kDa |
| Gene Summary | This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205351L1 | Lenti ORF clone of Human heat shock 70kDa protein 1-like (HSPA1L), Myc-DDK-tagged |
CNY 9,560.00 |
|
| RC205351L2 | Lenti ORF clone of Human heat shock 70kDa protein 1-like (HSPA1L), mGFP tagged |
CNY 9,560.00 |
|
| RC205351L3 | Lenti ORF clone of Human heat shock 70kDa protein 1-like (HSPA1L), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205351L4 | Lenti ORF clone of Human heat shock 70kDa protein 1-like (HSPA1L), mGFP tagged |
CNY 5,890.00 |
|
| RG205351 | HSPA1L (tGFP-tagged) - Human heat shock 70kDa protein 1-like (HSPA1L) |
CNY 8,760.00 |
|
| SC110926 | HSPA1L (untagged)-Human heat shock 70kDa protein 1-like (HSPA1L) |
CNY 4,784.00 |
