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KCNK1 (NM_002245) Human Tagged ORF Clone
CAT＃: RC205321
KCNK1 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 1 (KCNK1)
ORF Plasmid: tGFP
"NM_002245" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DPK; HOHO; K2P1; K2p1.1; KCNO1; TWIK-1; TWIK1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205321 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAGTCCCTGGCCGGCAGCTCGTGCGTGCGCCTGGTGGAGCGGCACCGCTCGGCCTGGTGCTTCG GCTTCCTGGTGCTGGGCTACTTGCTCTACCTGGTCTTCGGCGCAGTGGTCTTCTCCTCGGTGGAGCTGCC CTATGAGGACCTGCTGCGCCAGGAGCTGCGCAAGCTGAAGCGACGCTTCTTGGAGGAGCACGAGTGCCTG TCTGAGCAGCAGCTGGAGCAGTTCCTGGGCCGGGTGCTGGAGGCCAGCAACTACGGCGTGTCGGTGCTCA GCAACGCCTCGGGCAACTGGAACTGGGACTTCACCTCCGCGCTCTTCTTCGCCAGCACCGTGCTCTCCAC CACAGGTTATGGCCACACCGTGCCCTTGTCAGATGGAGGTAAGGCCTTCTGCATCATCTACTCCGTCATT GGCATTCCCTTCACCCTCCTGTTCCTGACGGCTGTGGTCCAGCGCATCACCGTGCACGTCACCCGCAGGC CGGTCCTCTACTTCCACATCCGCTGGGGCTTCTCCAAGCAGGTGGTGGCCATCGTCCATGCCGTGCTCCT TGGGTTTGTCACTGTGTCCTGCTTCTTCTTCATCCCGGCCGCTGTCTTCTCAGTCCTGGAGGATGACTGG AACTTCCTGGAATCCTTTTATTTTTGTTTTATTTCCCTGAGCACCATTGGCCTGGGGGATTATGTGCCTG GGGAAGGCTACAATCAAAAATTCAGAGAGCTCTATAAGATTGGGATCACGTGTTACCTGCTACTTGGCCT TATTGCCATGTTGGTAGTTCTGGAAACCTTCTGTGAACTCCATGAGCTGAAAAAATTCAGAAAAATGTTC TATGTGAAGAAGGACAAGGACGAGGATCAGGTGCACATCATAGAGCATGACCAACTGTCATTCTCCTCGA TCACAGACCAGGCAGCTGGCATGAAAGAGGACCAGAAGCAAAATGAGCCTTTTGTGGCCACCCAGTCATC TGCCTGCGTGGATGGCCCTGCAAACCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205321 protein sequence
Red=Cloning site Green=Tags(s) MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKRRFLEEHECL SEQQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVI GIPFTLLFLTAVVQRITVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVFSVLEDDW NFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMF YVKKDKDEDQVHIIEHDQLSFSSITDQAAGMKEDQKQNEPFVATQSSACVDGPANH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002245 |
| ORF Size | 1008 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002245.4 |
| RefSeq Size | 2161 bp |
| RefSeq ORF | 1011 bp |
| Locus ID | 3775 |
| UniProt ID | O00180 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 38.1 kDa |
| Gene Summary | This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205321L3 | Lenti ORF clone of Human potassium channel, subfamily K, member 1 (KCNK1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205321L4 | Lenti ORF clone of Human potassium channel, subfamily K, member 1 (KCNK1), mGFP tagged |
CNY 5,890.00 |
|
| RG205321 | KCNK1 (tGFP-tagged) - Human potassium channel, subfamily K, member 1 (KCNK1) |
CNY 5,256.00 |
|
| SC110937 | KCNK1 (untagged)-Human potassium channel, subfamily K, member 1 (KCNK1) |
CNY 3,656.00 |
