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ARTS1 (ERAP1) (NM_001040458) Human Tagged ORF Clone
CAT＃: RC205249
ERAP1 (Myc-DDK-tagged)-Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2
ORF Plasmid: tGFP
"NM_001040458" in other vectors (6)
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CNY 6,896.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A-LAP; ALAP; APPILS; ARTS-1; ARTS1; ERAAP; ERAAP1; PILS-AP; PILSAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205249 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTTCTGCCCCTCAAATGGTCCCTTGCAACCATGTCATTTCTACTTTCCTCACTGTTGGCTCTCT TAACTGTGTCCACTCCTTCATGGTGTCAGAGCACTGAAGCATCTCCAAAACGTAGTGATGGGACACCATT TCCTTGGAATAAAATACGACTTCCTGAGTACGTCATCCCAGTTCATTATGATCTCTTGATCCATGCAAAC CTTACCACGCTGACCTTCTGGGGAACCACGAAAGTAGAAATCACAGCCAGTCAGCCCACCAGCACCATCA TCCTGCATAGTCACCACCTGCAGATATCTAGGGCCACCCTCAGGAAGGGAGCTGGAGAGAGGCTATCGGA AGAACCCCTGCAGGTCCTGGAACACCCCCGTCAGGAGCAAATTGCACTGCTGGCTCCCGAGCCCCTCCTT GTCGGGCTCCCGTACACAGTTGTCATTCACTATGCTGGCAATCTTTCGGAGACTTTCCACGGATTTTACA AAAGCACCTACAGAACCAAGGAAGGGGAACTGAGGATACTAGCATCAACACAATTTGAACCCACTGCAGC TAGAATGGCCTTTCCCTGCTTTGATGAACCTGCCTTCAAAGCAAGTTTCTCAATCAAAATTAGAAGAGAG CCAAGGCACCTAGCCATCTCCAATATGCCATTGGTGAAATCTGTGACTGTTGCTGAAGGACTCATAGAAG ACCATTTTGATGTCACTGTGAAGATGAGCACCTATCTGGTGGCCTTCATCATTTCAGATTTTGAGTCTGT CAGCAAGATAACCAAGAGTGGAGTCAAGGTTTCTGTTTATGCTGTGCCAGACAAGATAAATCAAGCAGAT TATGCACTGGATGCTGCGGTGACTCTTCTAGAATTTTATGAGGATTATTTCAGCATACCGTATCCCCTAC CCAAACAAGATCTTGCTGCTATTCCCGACTTTCAGTCTGGTGCTATGGAAAACTGGGGACTGACAACATA TAGAGAATCTGCTCTGTTGTTTGATGCAGAAAAGTCTTCTGCATCAAGTAAGCTTGGCATCACAATGACT GTGGCCCATGAACTGGCTCACCAGTGGTTTGGGAACCTGGTCACTATGGAATGGTGGAATGATCTTTGGC TAAATGAAGGATTTGCCAAATTTATGGAGTTTGTGTCTGTCAGTGTGACCCATCCTGAACTGAAAGTTGG AGATTATTTCTTTGGCAAATGTTTTGACGCAATGGAGGTAGATGCTTTAAATTCCTCACACCCTGTGTCT ACACCTGTGGAAAATCCTGCTCAGATCCGGGAGATGTTTGATGATGTTTCTTATGATAAGGGAGCTTGTA TTCTGAATATGCTAAGGGAGTATCTTAGTGCTGACGCATTTAAAAGTGGTATTGTACAGTATCTCCAGAA GCATAGCTATAAAAATACAAAAAACGAGGACCTGTGGGATAGTATGGCAAGTATTTGCCCTACAGATGGT GTAAAAGGGATGGATGGCTTTTGCTCTAGAAGTCAACATTCATCTTCATCCTCACATTGGCATCAGGAAG GGGTGGATGTGAAAACCATGATGAACACTTGGACACTGCAGAAGGGTTTTCCCCTAATAACCATCACAGT GAGGGGGAGGAATGTACACATGAAGCAAGAGCACTACATGAAGGGCTCTGACGGCGCCCCGGACACTGGG TACCTGTGGCATGTTCCATTGACATTCATCACCAGCAAATCCGACATGGTCCATCGATTTTTGCTAAAAA CAAAAACAGATGTGCTCATCCTCCCAGAAGAGGTGGAATGGATCAAATTTAATGTGGGCATGAATGGCTA TTACATTGTGCATTACGAGGATGATGGATGGGACTCTTTGACTGGCCTTTTAAAAGGAACACACACAGCA GTCAGCAGTAATGATCGGGCGAGTCTCATTAACAATGCATTTCAGCTCGTCAGCATTGGGAAGCTGTCCA TTGAAAAGGCCTTGGATTTATCCCTGTACTTGAAACATGAAACTGAAATTATGCCCGTGTTTCAAGGTTT GAATGAGCTGATTCCTATGTATAAGTTAATGGAGAAAAGAGATATGAATGAAGTGGAAACTCAATTCAAG GCCTTCCTCATCAGGCTGCTAAGGGACCTCATTGATAAGCAGACATGGACAGACGAGGGCTCAGTCTCAG AGCGAATGCTGCGGAGTCAACTACTACTCCTCGCCTGTGTGCACAACTATCAGCCGTGCGTACAGAGGGC AGAAGGCTATTTCAGAAAGTGGAAGGAATCCAATGGAAACTTGAGCCTGCCTGTCGACGTGACCTTGGCA GTGTTTGCTGTGGGGGCCCAGAGCACAGAAGGCTGGGATTTTCTTTATAGTAAATATCAGTTTTCTTTGT CCAGTACTGAGAAAAGCCAAATTGAATTTGCCCTCTGCAGAACCCAAAATAAGGAAAAGCTTCAATGGCT ACTAGATGAAAGCTTTAAGGGAGATAAAATAAAAACTCAGGAGTTTCCACAAATTCTTACACTCATTGGC AGGAACCCAGTAGGATACCCACTGGCCTGGCAATTTCTGAGGAAAAACTGGAACAAACTTGTACAAAAGT TTGAACTTGGCTCATCTTCCATAGCCCACATGGTAATGGGTACAACAAATCAATTCTCCACAAGAACACG GCTTGAAGAGGTAAAAGGATTCTTCAGCTCTTTGAAAGAAAATGGTTCTCAGCTCCGTTGTGTCCAACAG ACAATTGAAACCATTGAAGAAAACATCGGTTGGATGGATAAGAATTTTGATAAAATCAGAGTGTGGCTGC AAAGTGAAAAGCTTGAACGTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205249 protein sequence
Red=Cloning site Green=Tags(s) MVFLPLKWSLATMSFLLSSLLALLTVSTPSWCQSTEASPKRSDGTPFPWNKIRLPEYVIPVHYDLLIHAN LTTLTFWGTTKVEITASQPTSTIILHSHHLQISRATLRKGAGERLSEEPLQVLEHPRQEQIALLAPEPLL VGLPYTVVIHYAGNLSETFHGFYKSTYRTKEGELRILASTQFEPTAARMAFPCFDEPAFKASFSIKIRRE PRHLAISNMPLVKSVTVAEGLIEDHFDVTVKMSTYLVAFIISDFESVSKITKSGVKVSVYAVPDKINQAD YALDAAVTLLEFYEDYFSIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESALLFDAEKSSASSKLGITMT VAHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVSVTHPELKVGDYFFGKCFDAMEVDALNSSHPVS TPVENPAQIREMFDDVSYDKGACILNMLREYLSADAFKSGIVQYLQKHSYKNTKNEDLWDSMASICPTDG VKGMDGFCSRSQHSSSSSHWHQEGVDVKTMMNTWTLQKGFPLITITVRGRNVHMKQEHYMKGSDGAPDTG YLWHVPLTFITSKSDMVHRFLLKTKTDVLILPEEVEWIKFNVGMNGYYIVHYEDDGWDSLTGLLKGTHTA VSSNDRASLINNAFQLVSIGKLSIEKALDLSLYLKHETEIMPVFQGLNELIPMYKLMEKRDMNEVETQFK AFLIRLLRDLIDKQTWTDEGSVSERMLRSQLLLLACVHNYQPCVQRAEGYFRKWKESNGNLSLPVDVTLA VFAVGAQSTEGWDFLYSKYQFSLSSTEKSQIEFALCRTQNKEKLQWLLDESFKGDKIKTQEFPQILTLIG RNPVGYPLAWQFLRKNWNKLVQKFELGSSSIAHMVMGTTNQFSTRTRLEEVKGFFSSLKENGSQLRCVQQ TIETIEENIGWMDKNFDKIRVWLQSEKLERM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040458 |
| ORF Size | 2823 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001040458.3 |
| RefSeq Size | 5085 bp |
| RefSeq ORF | 2826 bp |
| Locus ID | 51752 |
| UniProt ID | Q9NZ08 |
| Protein Families | Druggable Genome, Protease, Secreted Protein |
| MW | 107.2 kDa |
| Gene Summary | The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205249L1 | Lenti ORF clone of Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205249L2 | Lenti ORF clone of Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2, mGFP tagged |
CNY 9,296.00 |
|
| RC205249L3 | Lenti ORF clone of Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205249L4 | Lenti ORF clone of Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG205249 | ERAP1 (tGFP-tagged) - Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2 |
CNY 8,496.00 |
|
| SC311137 | ERAP1 (untagged)-Human endoplasmic reticulum aminopeptidase 1 (ERAP1), transcript variant 2 |
CNY 6,896.00 |
