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MDM1 (NM_017440) Human Tagged ORF Clone
CAT＃: RC205187
MDM1 (Myc-DDK-tagged)-Human Mdm1 nuclear protein homolog (mouse) (MDM1), transcript variant 1
ORF Plasmid: tGFP
"NM_017440" in other vectors (4)
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CNY 5,232.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205187 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGCGCTTCAAGGGGCTGAGTGAATACCAGAGGAACTTCCTGTGGAAAAAGTCTTATTTGTCAG AGTCTTGTAATTCCTCCGTGGGGCGAAAGTACCCATGGGCTGGACTTAGATCAGATCAATTAGGCATCAC GAAAGAGCCAAGTTTTATTTCAAAAAGAAGAGTCCCTTACCATGACCCACAGATTTCAAAATCTCTGGAG TGGAATGGAGCTATCTCAGAGAGCAATGTGGTTGCATCACCAGAACCAGAAGCCCCGGAAACACCAAAAT CACAAGAAGCAGAACAAAAGGATGTTATTCAAGAAAGAGTTCACTCACTAGAAGCTTCCAGGGTTCCCAA AAGAACCAGATCTCACTCTGCAGACTCCAGAGCTGAAGGGGCTTCAGATGTGGAAAATAATGAGGGTGTA ACAAACCATACACCAGTTAATGAAAATGTGGAACTGGAACATTCTACCAAGGTTCTTTCAGAAAATGTAG ATAATGGGTTGGATAGACTTCTGCGTAAGAAAGCTGGATTGACTGTTGTTCCTTCATATAATGCCTTGAG AAATTCTGAATATCAAAGGCAGTTTGTTTGGAAGACTTCTAAAGAAACTGCTCCAGCTTTTGCAGCCAAT CAGGTTTTCCACAATAAAAGCCAGTTTGTTCCACCATTCAAAGGTAACTCAGTCATCCATGAAACTGAAT ACAAAAGAAATTTCAAGGGTTTATCTCCAGTGAAAGAACCAAAATTAAGAAATGATTTGAGAGAAAACAG AAATCTTGAAACAGTGTCTCCTGAAAGGAAGAGTAATAAAATAGACGATCGTTTAAAATTGGAAGCAGAG ATGGAATTAAAAGACTTACACCAGCCTAAAAGGAAGCTTACTCCTTGGAAACATCAAAGGCTTGGGAAGG TGAATTCCGAATATAGAGCAAAATTTCTGAGCCCAGCTCAGTATTTATATAAAGCTGGGGCTTGGACACA TGTAAAGGGAAACATGCCAAATCAGGTTAAAGAACTCCGAGAAAAGGCTGAGTTTTATAGGAAGCGAGTT CAGGGGACGCATTTTTCTCGGGACCATCTGAATCAGATTTTATCTGATAGCAACTGCTGTTGGGATGTCT CCTCAACCACAAGCTCAGAAGGAACCGTTAGTAGCAACATCAGAGCATTAGATCTTGCTGGAGATCCTAC AAGCCATAAGACTTTGCAGAAATGTCCTTCTACAGAACCAGAAGAAAAAGGAAATATCGTGGAAGAACAG CCCCAGAAAAATACCACGGAGAAATTGGGTGTGTCAGCTCCCACCATACCCGTTAGAAGGCGGCTGGCTT GGGATACAGAGAACACAAGTGAAGACGTACAGAAACAGCCCGGGGAGAAAGAGGAGGAGGACGACAATGA AGAGGAAGGGGACAGGAAAACGGGCAAGCAGGCTTTTATGGGAGAGCAAGAGAAGTTGGATGTACATGAG AAATCTAAGGCAGATAAGATGAAAGAAGGGTCAGATTCTTCTGTATCCTCAGAAAAAGGAGGCCGGCTTC CTACTCCCAAGCTGAGAGAACTTGGTGGAATCCAGAGGACTCATCATGATCTCACTACTCCAGCTGTTGG TGGTGCTGTTTTAGTGTCTCCATCTAAGATGAAGCCTCCAGCCCTAGAACAGAGGAAAAGAATGACCTCT CAGGATTGTTTAGAAACTTCAAAGAATGATTTTACTAAGAAAGAAAGTCGTGCTGTATCCCTACTGACTT CTCCAGCTGCTGGTATAAAAACAGTTGATCCTCTGCCTTTGCGGGAAGATTCTGAAGACAATATCCACAA ATTTGCTGAGGCAACTCTTCCAGTTTCAAAAATTCCAAAATACCCAACAAATCCCCCTGGACAGTTGCCT TCTCCACCACATGTTCCATCCTACTGGCATCCCTCTCGACGAATTCAGGGCTCTCTTAGAGATCCAGAGT TTCAGCACAATGTGGGAAAAGCAAGGATGAACAATTTGCAGTTACCTCAACATGAAGCCTTTAATGATGA AGATGAGGACAGATTGTCTGAGATTTCTGCTCGCTCTGCAGCTTCTAGTCTCCGGGCTTTTCAAACTCTG GCACGAGCTAAGAAAAGGAAGGAGAATTTCTGGGGTAAAACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205187 protein sequence
Red=Cloning site Green=Tags(s) MPVRFKGLSEYQRNFLWKKSYLSESCNSSVGRKYPWAGLRSDQLGITKEPSFISKRRVPYHDPQISKSLE WNGAISESNVVASPEPEAPETPKSQEAEQKDVIQERVHSLEASRVPKRTRSHSADSRAEGASDVENNEGV TNHTPVNENVELEHSTKVLSENVDNGLDRLLRKKAGLTVVPSYNALRNSEYQRQFVWKTSKETAPAFAAN QVFHNKSQFVPPFKGNSVIHETEYKRNFKGLSPVKEPKLRNDLRENRNLETVSPERKSNKIDDRLKLEAE MELKDLHQPKRKLTPWKHQRLGKVNSEYRAKFLSPAQYLYKAGAWTHVKGNMPNQVKELREKAEFYRKRV QGTHFSRDHLNQILSDSNCCWDVSSTTSSEGTVSSNIRALDLAGDPTSHKTLQKCPSTEPEEKGNIVEEQ PQKNTTEKLGVSAPTIPVRRRLAWDTENTSEDVQKQPGEKEEEDDNEEEGDRKTGKQAFMGEQEKLDVHE KSKADKMKEGSDSSVSSEKGGRLPTPKLRELGGIQRTHHDLTTPAVGGAVLVSPSKMKPPALEQRKRMTS QDCLETSKNDFTKKESRAVSLLTSPAAGIKTVDPLPLREDSEDNIHKFAEATLPVSKIPKYPTNPPGQLP SPPHVPSYWHPSRRIQGSLRDPEFQHNVGKARMNNLQLPQHEAFNDEDEDRLSEISARSAASSLRAFQTL ARAKKRKENFWGKT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017440 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017440.3 |
| RefSeq Size | 2976 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 56890 |
| UniProt ID | Q8TC05 |
| Protein Families | Druggable Genome |
| MW | 80.7 kDa |
| Gene Summary | This gene encodes a microtubule-binding nuclear protein that localizes to the centrioles of dividing cells and differentiating multiciliated cells and negatively regulates centriole duplication. The encoded protein is closely associated with the centriole barrel, and resides in the centriole lumen. Naturally-occurring mutations in the orthologous mouse gene are associated with age-related retinal degeneration. [provided by RefSeq, Feb 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205187L3 | Lenti ORF clone of Human Mdm1 nuclear protein homolog (mouse) (MDM1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205187L4 | Lenti ORF clone of Human Mdm1 nuclear protein homolog (mouse) (MDM1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG205187 | MDM1 (tGFP-tagged) - Human Mdm1 nuclear protein homolog (mouse) (MDM1), transcript variant 1 |
CNY 6,832.00 |
|
| SC100908 | MDM1 (untagged)-Human Mdm1 nuclear protein homolog (mouse) (MDM1), transcript variant 1 |
CNY 5,240.00 |
