United States
Germany
Japan
United Kingdom
China
COQ10B (NM_025147) Human Tagged ORF Clone
CAT＃: RC205174
COQ10B (Myc-DDK-tagged)-Human coenzyme Q10 homolog B (S. cerevisiae) (COQ10B), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_025147" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205174 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTCGGACTGGTCATACGGCCTTGAGAAGGGTAGTCTCGGGATGCCGTCCGAAGTCGGCGACAG CGGCCGGGGCGCAGGCGCCCGTGCGGAATGGCAGATATTTAGCTTCCTGTGGTATACTGATGAGCAGAAC TCTTCCACTACATACCTCAATTTTGCCTAAGGAGATATGTGCACGAACTTTCTTCAAAATCACTGCACCA TTAATAAACAAAAGGAAAGAATATTCAGAGAGAAGAATTTTAGGATATTCAATGCAGGAAATGTATGATG TAGTATCGGGAGTGGAGGATTACAAGCATTTTGTTCCTTGGTGCAAAAAATCAGATGTTATATCAAAGAG ATCTGGATATTGTAAAACAAGATTAGAAATTGGATTTCCACCTGTGTTGGAGCGATATACATCAGTAGTA ACCTTGGTGAAACCTCATTTAGTAAAGGCATCTTGTACTGATGGGAGACTTTTCAATCATTTGGAGACTA TTTGGCGTTTTAGCCCAGGTCTTCCTGGCTACCCAAGAACTTGTACCTTGGATTTTTCAATTTCTTTTGA ATTTCGATCACTTCTACATTCCCAGCTTGCCACACTCTTTTTTGATGAAGTTGTGAAGCAGATGGTAGCT GCCTTTGAAAGAAGAGCATGTAAGCTGTATGGTCCAGAAACAAATATACCTCGGGAGTTAATGCTTCATG AAGTCCATCACACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205174 protein sequence
Red=Cloning site Green=Tags(s) MAARTGHTALRRVVSGCRPKSATAAGAQAPVRNGRYLASCGILMSRTLPLHTSILPKEICARTFFKITAP LINKRKEYSERRILGYSMQEMYDVVSGVEDYKHFVPWCKKSDVISKRSGYCKTRLEIGFPPVLERYTSVV TLVKPHLVKASCTDGRLFNHLETIWRFSPGLPGYPRTCTLDFSISFEFRSLLHSQLATLFFDEVVKQMVA AFERRACKLYGPETNIPRELMLHEVHHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025147 |
| ORF Size | 714 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025147.4 |
| RefSeq Size | 1989 bp |
| RefSeq ORF | 717 bp |
| Locus ID | 80219 |
| UniProt ID | Q9H8M1 |
| Domains | ARPF |
| MW | 27.1 kDa |
| Gene Summary | Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205174L3 | Lenti ORF clone of Human coenzyme Q10 homolog B (S. cerevisiae) (COQ10B), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205174L4 | Lenti ORF clone of Human coenzyme Q10 homolog B (S. cerevisiae) (COQ10B), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG205174 | COQ10B (tGFP-tagged) - Human coenzyme Q10 homolog B (S. cerevisiae) (COQ10B), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC111068 | COQ10B (untagged)-Human coenzyme Q10 homolog B (S. cerevisiae) (COQ10B), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
