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ADAM20 (NM_003814) Human Tagged ORF Clone
CAT＃: RC205168
ADAM20 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 20 (ADAM20)
ORF Plasmid: tGFP
"NM_003814" in other vectors (4)
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CNY 5,688.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205168 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCAGCTCCACCAGGACACAGATCCCCAGATCCCTAAAGGTCAGCCATGCACCCTGAACAGCTCAG AGGGAGGAGCCAGACCAGCAGTGCCTCACACCTTGTTCTCTTCTGCTCTAGACAGATGGCTCCATAATGA CAGCTTCATAATGGCAGTGGGTGAGCCCCTGGTGCACATCAGGGTCACTCTTCTGCTGCTCTGGTTTGGG ATGTTTTTGTCTATTTCTGGCCACTCTCAGGCCAGGCCCTCCCAGTATTTCACTTCTCCAGAAGTGGTGA TCCCTTTGAAGGTGATCAGCAGGGGCAGAGGTGCAAAGGCTCCTGGATGGCTCTCCTATAGCCTGCGGTT TGGGGGACAGAGATACATTGTCCACATGAGGGTAAATAAGCTGTTGTTTGCTGCACACCTTCCTGTGTTC ACCTACACAGAGCAGCATGCCCTGCTCCAGGATCAGCCCTTCATCCAGGATGACTGCTACTACCATGGTT ATGTGGAGGGGGTCCCTGAGTCCTTGGTTGCCCTTAGTACCTGTTCTGGGGGCTTTCTTGGAATGCTACA GATAAATGACCTTGTTTATGAAATCAAGCCAATTAGTGTTTCTGCCACATTTGAACACCTAGTATATAAG ATAGACAGTGATGATACACAGTTTCCACCTATGAGATGTGGGTTAACAGAAGAGAAAATAGCACACCAGA TGGAGTTGCAATTGTCATATAATTTCACTCTGAAGCAAAGTTCTTTTGTGGGCTGGTGGACCCATCAGCG GTTTGTTGAGCTGGTAGTGGTCGTGGATAATATTAGATATCTTTTCTCTCAAAGTAATGCAACAACAGTG CAGCATGAAGTATTTAACGTTGTCAATATAGTGGATTCCTTCTATCATCCTTTGGAGGTTGATGTAATTT TGACTGGAATTGATATATGGACTGCATCAAATCCACTTCCTACCAGTGGAGACCTAGATAATGTTTTAGA GGACTTTTCTATTTGGAAGAATTATAACCTTAATAATCGACTACAACATGATGTTGCACATCTTTTCATA AAAGACACACAAGGCATGAAGCTTGGTGTTGCCTATGTTAAAGGAATATGCCAGAATCCTTTTAATACTG GAGTTGATGTTTTTGAAGACAACAGGTTGGTCGTTTTTGCAATTACTTTGGGCCACGAGCTTGGTCATAA TTTGGGTATGCAACATGACACCCAGTGGTGTGTGTGCGAGCTACAGTGGTGCATAATGCATGCCTATAGA AAGGTGACAACTAAATTTAGCAACTGCAGTTATGCCCAATATTGGGACAGTACTATCAGTAGTGGATTAT GTATTCAACCGCCTCCATATCCAGGGAATATATTTAGACTGAAGTACTGTGGGAATCTAGTGGTTGAAGA AGGGGAGGAATGTGACTGTGGAACCATACGGCAGTGTGCAAAAGATCCCTGTTGTCTGTTAAACTGTACT CTACATCCTGGGGCTGCTTGTGCTTTTGGAATATGTTGCAAAGACTGCAAATTTCTGCCATCAGGAACTT TATGTAGACAACAAGTTGGTGAATGTGACCTTCCAGAGTGGTGCAATGGGACATCCCATCAATGCCCAGA TGATGTGTATGTGCAGGACGGGATCTCCTGTAATGTGAATGCCTTCTGCTATGAAAAGACGTGTAATAAC CATGATATACAATGTAAAGAGATTTTTGGCCAAGATGCAAGGAGTGCATCTCAGAGTTGCTACCAAGAAA TCAACACCCAAGGAAACCGTTTCGGTCACTGTGGTATTGTAGGCACAACATATGTAAAATGTTGGACCCC TGATATCATGTGTGGGAGGGTTCAGTGTGAAAATGTGGGAGTAATTCCCAATCTGATAGAGCATTCTACA GTGCAGCAGTTTCACCTCAATGACACCACTTGCTGGGGCACTGATTATCATTTAGGGATGGCTATACCTG ATATTGGTGAGGTGAAAGATGGCACAGTATGTGGTCCAGAAAAGATCTGCATCCGTAAGAAGTGTGCCAG TATGGTTCATCTGTCACAAGCCTGTCAGCCTAAGACCTGCAACATGAGGGGAATCTGCAACAACAAACAA CACTGTCACTGCAACCATGAATGGGCACCCCCATACTGCAAGGACAAAGGCTATGGAGGTAGTGCTGATA GTGGCCCACCTCCTAAGAACAACATGGAAGGATTAAATGTGATGGGAAAGTTGCGTTACCTGTCACTATT GTGCCTTCTTCCTTTGGTTGCTTTTTTATTATTTTGCTTACATGTGCTTTTTAAGAAACGCACAAAAAGT AAAGAAGATGAAGAAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205168 protein sequence
Red=Cloning site Green=Tags(s) MVQLHQDTDPQIPKGQPCTLNSSEGGARPAVPHTLFSSALDRWLHNDSFIMAVGEPLVHIRVTLLLLWFG MFLSISGHSQARPSQYFTSPEVVIPLKVISRGRGAKAPGWLSYSLRFGGQRYIVHMRVNKLLFAAHLPVF TYTEQHALLQDQPFIQDDCYYHGYVEGVPESLVALSTCSGGFLGMLQINDLVYEIKPISVSATFEHLVYK IDSDDTQFPPMRCGLTEEKIAHQMELQLSYNFTLKQSSFVGWWTHQRFVELVVVVDNIRYLFSQSNATTV QHEVFNVVNIVDSFYHPLEVDVILTGIDIWTASNPLPTSGDLDNVLEDFSIWKNYNLNNRLQHDVAHLFI KDTQGMKLGVAYVKGICQNPFNTGVDVFEDNRLVVFAITLGHELGHNLGMQHDTQWCVCELQWCIMHAYR KVTTKFSNCSYAQYWDSTISSGLCIQPPPYPGNIFRLKYCGNLVVEEGEECDCGTIRQCAKDPCCLLNCT LHPGAACAFGICCKDCKFLPSGTLCRQQVGECDLPEWCNGTSHQCPDDVYVQDGISCNVNAFCYEKTCNN HDIQCKEIFGQDARSASQSCYQEINTQGNRFGHCGIVGTTYVKCWTPDIMCGRVQCENVGVIPNLIEHST VQQFHLNDTTCWGTDYHLGMAIPDIGEVKDGTVCGPEKICIRKKCASMVHLSQACQPKTCNMRGICNNKQ HCHCNHEWAPPYCKDKGYGGSADSGPPPKNNMEGLNVMGKLRYLSLLCLLPLVAFLLFCLHVLFKKRTKS KEDEEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003814 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003814.4, NP_003805.3 |
| RefSeq Size | 2807 bp |
| RefSeq ORF | 2181 bp |
| Locus ID | 8748 |
| UniProt ID | O43506 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 87.1 kDa |
| Gene Summary | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The expression of this gene is testis-specific. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205168L3 | Lenti ORF clone of Human ADAM metallopeptidase domain 20 (ADAM20), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC205168L4 | Lenti ORF clone of Human ADAM metallopeptidase domain 20 (ADAM20), mGFP tagged |
CNY 7,700.00 |
|
| RG205168 | ADAM20 (tGFP-tagged) - Human ADAM metallopeptidase domain 20 (ADAM20) |
CNY 6,370.00 |
|
| SC117728 | ADAM20 (untagged)-Human ADAM metallopeptidase domain 20 (ADAM20) |
CNY 7,220.00 |
