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PHAPI2 / APRIL (ANP32B) (NM_006401) Human Tagged ORF Clone
CAT＃: RC205158
ANP32B (Myc-DDK-tagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B)
ORF Plasmid: tGFP
"NM_006401" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APRIL; PHAPI2; SSP29 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC205158 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATGAAGAGGAGGATCCACCTGGAGCTGAGGAACCGGACCCCGGCAGCTGTTCGAGAACTTGTCT TGGACAATTGCAAATCAAATGATGGAAAAATTGAGGGCTTAACAGCTGAATTTGTGAACTTAGAGTTCCT CAGTTTAATAAATGTAGGCTTGATCTCAGTTTCAAATCTCCCCAAGCTGCCTAAATTGAAAAAGCTTGAA CTCAGTGAAAATAGAATCTTTGGAGGTCTGGACATGTTAGCTGAAAAACTTCCAAATCTCACACATCTAA ACTTAAGTGGAAATAAACTGAAAGATATCAGCACCTTGGAACCTTTGAAAAAGTTAGAATGTCTGAAAAG CCTGGACCTCTTTAACTGTGAGGTTACCAACCTGAATGACTACCGAGAGAGTGTCTTCAAGCTCCTGCCC CAGCTTACCTACTTGGATGGCTATGACCGAGAGGACCAGGAAGCACCTGACTCAGATGCCGAGGTGGATG GTGTGGATGAAGAGGAGGAGGACGAAGAAGGAGAAGATGAGGAAGACGAGGACGATGAGGATGGTGAAGA AGAGGAGTTTGATGAAGAAGATGATGAAGATGAAGATGTAGAAGGGGATGAGGACGACGATGAAGTCAGT GAGGAGGAAGAAGAATTTGGACTTGATGAAGAAGATGAAGATGAGGATGAGGATGAAGAGGAGGAAGAAG GTGGGAAAGGTGAAAAGAGGAAGAGAGAAACAGATGATGAAGGAGAAGATGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205158 protein sequence
Red=Cloning site Green=Tags(s) MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLE LSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLP QLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVS EEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006401 |
| ORF Size | 753 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006401.3 |
| RefSeq Size | 1617 bp |
| RefSeq ORF | 756 bp |
| Locus ID | 10541 |
| UniProt ID | Q92688 |
| Domains | LRR, LRRcap |
| MW | 28.8 kDa |
| Gene Summary | Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205158L1 | Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC205158L2 | Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), mGFP tagged |
CNY 5,890.00 |
|
| RC205158L3 | Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205158L4 | Lenti ORF clone of Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B), mGFP tagged |
CNY 5,890.00 |
|
| RG205158 | ANP32B (tGFP-tagged) - Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B) |
CNY 4,000.00 |
|
| SC116090 | ANP32B (untagged)-Human acidic (leucine-rich) nuclear phosphoprotein 32 family, member B (ANP32B) |
CNY 2,400.00 |
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