United States
Germany
Japan
United Kingdom
China
TH1L (NELFCD) (NM_198976) Human Tagged ORF Clone
CAT＃: RC204842
NELFCD (Myc-DDK-tagged)-Human TH1-like (Drosophila) (TH1L), transcript variant 1
ORF Plasmid: tGFP
"NM_198976" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,392.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HSPC130; NELF-C; NELF-D; TH1; TH1L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204842 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGGCCGTGCCGGGCGCCATCATGGACGAGGACTACTACGGGAGCGCGGCCGAGTGGGGCGACG AGGCTGACGGCGGCCAGCAGGAGGATGATTCTGGAGAAGGAGAGGATGATGCGGAGGTTCAGCAAGAATG CCTGCATAAATTTTCCACCCGGGATTATATCATGGAACCCTCCATCTTCAACACTCTGAAGAGGTATTTT CAGGCAGGAGGGTCTCCAGAGAATGTTATCCAGCTCTTATCTGAAAACTACACCGCTGTGGCCCAGACTG TGAACCTGCTGGCCGAGTGGCTCATTCAGACAGGTGTTGAGCCAGTGCAGGTTCAGGAAACTGTGGAAAA TCACTTGAAGAGTTTGCTGATCAAACATTTTGACCCCCGCAAAGCAGATTCTATTTTTACTGAAGAAGGA GAGACCCCAGCGTGGCTGGAACAGATGATTGCACATACCACGTGGCGGGACCTTTTTTATAAACTGGCTG AAGCCCATCCAGACTGTTTGATGCTGAACTTCACCGTTAAGCTTATTTCTGACGCAGGGTACCAGGGGGA GATCACCAGTGTGTCCACAGCATGCCAGCAGCTAGAAGTGTTCTCGAGAGTGCTCCGGACCTCTCTAGCT ACAATTTTAGATGGAGGAGAAGAAAACCTTGAAAAAAATCTCCCTGAGTTTGCTAAGATGGTGTGCCACG GGGAGCACACGTACCTGTTTGCCCAGGCCATGATGTCCGTGCTGGCCCAGGAGGAGCAGGGGGGCTCCGC TGTGCGCAGGATCGCCCAGGAAGTGCAGCGCTTTGCCCAGGAGAAAGGTCATGACGCCAGTCAGATCACA CTAGCCTTGGGCACAGCTGCCTCCTACCCCAGGGCCTGCCAGGCTCTCGGGGCCATGCTGTCCAAAGGAG CCCTGAACCCTGCTGACATCACCGTCCTGTTCAAGATGTTCACAAGCATGGACCCTCCTCCGGTTGAACT TATCCGCGTTCCAGCCTTCCTGGACCTGTTCATGCAGTCACTCTTTAAACCAGGGGCTCGGATCAACCAG GACCACAAGCACAAATACATCCACATCTTGGCGTACGCAGCAAGCGTGGTTGAGACCTGGAAGAAGAACA AGCGAGTGAGCATCAATAAAGATGAGCTGAAGTCAACGTCAAAAGCTGTCGAAACCGTTCACAATTTGTG TTGCAACGAGAACAAAGGGGCCTCTGAACTAGTGGCAGAATTGAGCACACTTTATCAGTGTATTAGGTTT CCAGTGGTAGCAATGGGTGTGCTGAAGTGGGTGGATTGGACTGTATCAGAACCAAGGTACTTTCAGCTGC AGACTGACCATACCCCTGTCCACCTGGCGTTGCTGGATGAGATCAGCACCTGCCACCAGCTCCTGCACCC CCAGGTCCTGCAGCTGCTTGTTAAGCTTTTTGAGACTGAGCACTCCCAGCTGGACGTGATGGAGCAGCTT GAGTTGAAGAAGACACTGCTGGACAGGATGGTTCACCTGCTGAGTCGAGGTTATGTACTTCCTGTTGTCA GTTACATCCGAAAGTGTCTGGAGAAGCTGGACACTGACATTTCACTCATTCGCTATTTTGTCACTGAGGT GCTGGACGTCATTGCTCCTCCTTATACCTCTGACTTCGTGCAACTTTTCCTCCCCATCCTGGAGAATGAC AGCATCGCAGGTACCATCAAAACGGAAGGCGAGCATGACCCTGTGACGGAGTTTATAGCTCACTGCAAAT CTAACTTCATCATGGTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204842 protein sequence
Red=Cloning site Green=Tags(s) MAGAVPGAIMDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRYF QAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEG ETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLA TILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQIT LALGTAASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQ DHKHKYIHILAYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRF PVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQL ELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILEND SIAGTIKTEGEHDPVTEFIAHCKSNFIMVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198976 |
| ORF Size | 1770 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198976.1, NP_945327.1 |
| RefSeq Size | 2320 bp |
| RefSeq ORF | 1746 bp |
| Locus ID | 51497 |
| UniProt ID | Q8IXH7 |
| Protein Families | Transcription Factors |
| MW | 66.2 kDa |
| Gene Summary | The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The V81M variant of tyrosine hydroxylase is associated with more severe freezing of gait in Parkinson's disease
,Tekin, I;Carkaci-Salli, N;Lewis, MM;Mailman, RB;,
Parkinsonism Relat Disord Feb2016
,PubMed ID 26732803
[TH1L]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204842L3 | Lenti ORF clone of Human TH1-like (Drosophila) (TH1L), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204842L4 | Lenti ORF clone of Human TH1-like (Drosophila) (TH1L), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204842 | NELFCD (tGFP-tagged) - Human TH1-like (Drosophila) (TH1L), transcript variant 1 |
CNY 4,370.00 |
|
| SC307839 | NELFCD (untagged)-Human TH1-like (Drosophila) (TH1L), transcript variant 1 |
CNY 7,220.00 |
