Pancreatic Lipase (PNLIP) (NM_000936) Human Tagged ORF Clone
CAT#: RC204735
PNLIP (Myc-DDK-tagged)-Human pancreatic lipase (PNLIP)
ORF Plasmid: tGFP
"NM_000936" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PL; PNLIPD; PTL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204735 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCACTTTGGACTCTTTCACTGCTGCTGGGAGCAGTAGCAGGAAAAGAAGTTTGCTACGAAAGAC TCGGCTGCTTCAGTGATGACTCCCCATGGTCAGGAATTACGGAAAGACCCCTCCATATATTGCCTTGGTC TCCAAAAGATGTCAACACCCGCTTCCTCCTATATACTAATGAGAACCCAAACAACTTTCAAGAAGTTGCC GCAGATTCATCAAGCATCAGTGGCTCCAATTTCAAAACAAATAGAAAAACTCGCTTTATTATTCATGGAT TCATAGACAAGGGAGAAGAAAACTGGCTGGCCAATGTGTGCAAGAATCTGTTCAAGGTGGAAAGTGTGAA CTGTATCTGTGTGGACTGGAAAGGTGGCTCCCGAACTGGATACACACAAGCCTCGCAGAACATCAGGATC GTGGGAGCAGAAGTGGCATATTTTGTTGAATTTCTTCAGTCGGCGTTCGGTTACTCACCTTCCAACGTGC ATGTCATTGGCCACAGCCTGGGTGCCCACGCTGCTGGGGAGGCTGGAAGGAGAACCAATGGGACCATTGG ACGCATCACAGGGTTGGACCCAGCAGAACCTTGCTTTCAGGGCACACCTGAATTAGTCCGATTGGACCCC AGCGATGCCAAATTTGTGGATGTAATTCACACGGATGGTGCCCCCATAGTCCCCAATTTGGGGTTTGGAA TGAGCCAAGTCGTGGGCCACCTAGATTTCTTTCCAAATGGAGGAGTGGAAATGCCTGGATGTAAAAAGAA CATTCTCTCTCAGATTGTGGACATAGACGGAATCTGGGAAGGGACTCGAGACTTTGCGGCCTGTAATCAC TTAAGAAGCTACAAATATTACACTGATAGCATCGTCAACCCTGATGGCTTTGCTGGATTCCCCTGTGCCT CTTACAACGTCTTCACTGCAAACAAGTGTTTCCCTTGTCCAAGTGGAGGCTGCCCACAGATGGGTCACTA TGCTGATAGATATCCTGGGAAAACAAATGATGTGGGCCAGAAATTTTATCTAGACACTGGTGATGCCAGT AATTTTGCACGTTGGAGGTATAAGGTATCTGTCACACTGTCTGGAAAAAAGGTTACAGGACACATACTAG TTTCTTTGTTCGGAAATAAAGGAAACTCTAAGCAGTATGAAATTTTCAAGGGCACTCTCAAACCAGATAG TACTCATTCCAATGAATTTGACTCAGATGTGGATGTTGGGGACTTGCAGATGGTTAAATTTATTTGGTAT AACAATGTGATCAACCCAACTTTACCTAGAGTGGGAGCATCCAAGATTATAGTGGAGACAAATGTTGGAA AACAGTTCAACTTCTGTAGTCCAGAAACCGTCAGGGAGGAAGTTCTGCTCACCCTCACACCGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204735 protein sequence
Red=Cloning site Green=Tags(s) MLPLWTLSLLLGAVAGKEVCYERLGCFSDDSPWSGITERPLHILPWSPKDVNTRFLLYTNENPNNFQEVA ADSSSISGSNFKTNRKTRFIIHGFIDKGEENWLANVCKNLFKVESVNCICVDWKGGSRTGYTQASQNIRI VGAEVAYFVEFLQSAFGYSPSNVHVIGHSLGAHAAGEAGRRTNGTIGRITGLDPAEPCFQGTPELVRLDP SDAKFVDVIHTDGAPIVPNLGFGMSQVVGHLDFFPNGGVEMPGCKKNILSQIVDIDGIWEGTRDFAACNH LRSYKYYTDSIVNPDGFAGFPCASYNVFTANKCFPCPSGGCPQMGHYADRYPGKTNDVGQKFYLDTGDAS NFARWRYKVSVTLSGKKVTGHILVSLFGNKGNSKQYEIFKGTLKPDSTHSNEFDSDVDVGDLQMVKFIWY NNVINPTLPRVGASKIIVETNVGKQFNFCSPETVREEVLLTLTPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000936 |
ORF Size | 1395 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000936.3, NP_000927.1 |
RefSeq Size | 1502 bp |
RefSeq ORF | 1398 bp |
Locus ID | 5406 |
UniProt ID | P16233 |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Glycerolipid metabolism, Metabolic pathways |
MW | 51.2 kDa |
Gene Summary | This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204735L1 | Lenti ORF clone of Human pancreatic lipase (PNLIP), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC204735L2 | Lenti ORF clone of Human pancreatic lipase (PNLIP), mGFP tagged |
CNY 5,890.00 |
|
RC204735L3 | Lenti ORF clone of Human pancreatic lipase (PNLIP), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC204735L4 | Lenti ORF clone of Human pancreatic lipase (PNLIP), mGFP tagged |
CNY 5,890.00 |
|
RG204735 | PNLIP (tGFP-tagged) - Human pancreatic lipase (PNLIP) |
CNY 5,256.00 |
|
SC300155 | PNLIP (untagged)-Human pancreatic lipase (PNLIP) |
CNY 7,410.00 |
|
SC321085 | PNLIP (untagged)-Human pancreatic lipase (PNLIP) |
CNY 3,656.00 |
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