PSG6 (NM_001031850) Human Tagged ORF Clone

CAT#: RC204648

PSG6 (Myc-DDK-tagged)-Human pregnancy specific beta-1-glycoprotein 6 (PSG6), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001031850" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-PSG6 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms PSBG-6; PSBG-10; PSBG-12; PSG10; PSGGB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204648 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACCCCTCTCAGCCCCTCCCTGCACTCAGCACATCACCTGGAAGGGGCTCCTGCTCACAGCATCAC
TTTTAAACTTCTGGAACCTGCCCACCACTGCCCAAGTAATAATTGAAGCCAAGCCACCCAAAGTTTCCGA
GGGGAAGGATGTTCTTCTACTTGTCCACAATTTGCCCCAGAATCTTACTGGCTACATCTGGTACAAAGGG
CAAATGACGGACCTCTACCATTACATTACATCATATGTAGTACACGGTCAAATTATATATGGGCCTGCCT
ACAGTGGACGAGAAACAGTATATTCCAATGCATCCCTGCTGATCCAGAATGTCACACAGGAGGATGCAGG
ATCCTACACCTTACACATCATAAAGCGAGGCGATGGGACTGGAGGAGTAACTGGATATTTCACTGTCACC
TTATACTCGGAGACTCCCAAGCCCTCCATCTCCAGCAGCAACTTAAACCCCAGGGAGGTCATGGAGGCTG
TGCGCTTAATCTGTGATCCTGAGACTCCGGATGCAAGCTACCTGTGGTTGCTGAATGGTCAGAACCTCCC
TATGACTCACAGGTTGCAGCTGTCCAAAACCAACAGGACCCTCTATCTATTTGGTGTCACAAAGTATATT
GCAGGACCCTATGAATGTGAAATACGGAACCCAGTGAGTGCCAGCCGCAGTGACCCAGTCACCCTGAATC
TCCTCCCGAAGCTGCCCATGCCTTACATCACCATCAACAACTTAAACCCCAGGGAGAAGAAGGATGTGTT
AGCCTTCACCTGTGAACCTAAGAGTCGGAACTACACCTACATTTGGTGGCTAAATGGTCAGAGCCTCCCG
GTCAGTCCGAGGGTAAAGCGACCCATTGAAAACAGGATACTCATTCTACCCAGTGTCACGAGAAATGAAA
CAGGACCCTATCAATGTGAAATACGGGACCGATATGGTGGCATCCGCAGTAACCCAGTCACCCTGAATGT
CCTCTATGGTCCAGACCTCCCCAGAATTTACCCTTCATTCACCTATTACCGTTCAGGAGAAAACCTCGAC
TTGTCCTGCTTTGCGGACTCTAACCCACCGGCAGAGTATTCTTGGACAATTAATGGGAAGTTTCAGCTAT
CAGGACAAAAGCTCTTTATCCCCCAAATTACTACAAATCATAGCGGGCTCTATGCTTGCTCTGTTCGTAA
CTCAGCCACTGGCAAGGAAATCTCCAAATCCATGATAGTCAAAGTCTCTGGTCCCTGCCATGGAAACCAG
ACAGAGTCTCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204648 protein sequence
Red=Cloning site Green=Tags(s)

MGPLSAPPCTQHITWKGLLLTASLLNFWNLPTTAQVIIEAKPPKVSEGKDVLLLVHNLPQNLTGYIWYKG
QMTDLYHYITSYVVHGQIIYGPAYSGRETVYSNASLLIQNVTQEDAGSYTLHIIKRGDGTGGVTGYFTVT
LYSETPKPSISSSNLNPREVMEAVRLICDPETPDASYLWLLNGQNLPMTHRLQLSKTNRTLYLFGVTKYI
AGPYECEIRNPVSASRSDPVTLNLLPKLPMPYITINNLNPREKKDVLAFTCEPKSRNYTYIWWLNGQSLP
VSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSNPVTLNVLYGPDLPRIYPSFTYYRSGENLD
LSCFADSNPPAEYSWTINGKFQLSGQKLFIPQITTNHSGLYACSVRNSATGKEISKSMIVKVSGPCHGNQ
TESH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001031850
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001031850.4
RefSeq Size 1743 bp
RefSeq ORF 1275 bp
Locus ID 5675
UniProt ID Q00889
Protein Families Secreted Protein
MW 47.5 kDa
Gene Summary This gene is a member of the pregnancy-specific glycoprotein (PSG) gene family. The PSG genes are a subgroup of the carcinoembryonic antigen (CEA) family of immunoglobulin-like genes, and are found in a gene cluster at 19q13.1-q13.2 telomeric to another cluster of CEA-related genes. The PSG genes are expressed by placental trophoblasts and released into the maternal circulation during pregnancy, and are thought to be essential for maintenance of normal pregnancy. The protein encoded by this gene contains the Arg-Gly-Asp tripeptide associated with cellular adhesion and recognition. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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