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GPR84 (NM_020370) Human Tagged ORF Clone
CAT＃: RC204621
GPR84 (Myc-DDK-tagged)-Human G protein-coupled receptor 84 (GPR84)
ORF Plasmid: tGFP
"NM_020370" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EX33; GPCR4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204621 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAACAGCTCTGACGCCAACTTCTCCTGCTACCATGAGTCTGTGCTGGGCTATCGTTATGTTGCAG TTAGCTGGGGGGTGGTGGTGGCTGTGACAGGCACCGTGGGCAATGTGCTCACCCTACTGGCCTTGGCCAT CCAGCCCAAGCTCCGTACCCGATTCAACCTGCTCATAGCCAACCTCACACTGGCTGATCTCCTCTACTGC ACGCTCCTTCAGCCCTTCTCTGTGGACACCTACCTCCACCTGCACTGGCGCACCGGTGCCACCTTCTGCA GGGTATTTGGGCTCCTCCTTTTTGCCTCCAATTCTGTCTCCATCCTGACCCTCTGCCTCATCGCACTGGG ACGCTACCTCCTCATTGCCCACCCTAAGCTTTTTCCCCAAGTTTTCAGTGCCAAGGGGATAGTGCTGGCA CTGGTGAGCACCTGGGTTGTGGGCGTGGCCAGCTTTGCTCCCCTCTGGCCTATTTATATCCTGGTACCTG TAGTCTGCACCTGCAGCTTTGACCGCATCCGAGGCCGGCCTTACACCACCATCCTCATGGGCATCTACTT TGTGCTTGGGCTCAGCAGTGTTGGCATCTTCTATTGCCTCATCCACCGCCAGGTCAAACGAGCAGCACAG GCACTGGACCAATACAAGTTGCGACAGGCAAGCATCCACTCCAACCATGTGGCCAGGACTGATGAGGCCA TGCCTGGTCGTTTCCAGGAGCTGGACAGCAGGTTAGCATCAGGAGGACCCAGTGAGGGGATTTCATCTGA GCCAGTCAGTGCTGCCACCACCCAGACCCTGGAAGGGGACTCATCAGAAGTGGGAGACCAGATCAACAGC AAGAGAGCTAAGCAGATGGCAGAGAAAAGCCCTCCAGAAGCATCTGCCAAAGCCCAGCCAATTAAAGGAG CCAGAAGAGCTCCGGATTCTTCATCGGAATTTGGGAAGGTGACTCGAATGTGTTTTGCTGTGTTCCTCTG CTTTGCCCTGAGCTACATCCCCTTCTTGCTGCTCAACATTCTGGATGCCAGAGTCCAGGCTCCCCGGGTG GTCCACATGCTTGCTGCCAACCTCACCTGGCTCAATGGTTGCATCAACCCTGTGCTCTATGCAGCCATGA ACCGCCAATTCCGCCAAGCATATGGCTCCATTTTAAAAAGAGGGCCCCGGAGTTTCCATAGGCTCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204621 protein sequence
Red=Cloning site Green=Tags(s) MWNSSDANFSCYHESVLGYRYVAVSWGVVVAVTGTVGNVLTLLALAIQPKLRTRFNLLIANLTLADLLYC TLLQPFSVDTYLHLHWRTGATFCRVFGLLLFASNSVSILTLCLIALGRYLLIAHPKLFPQVFSAKGIVLA LVSTWVVGVASFAPLWPIYILVPVVCTCSFDRIRGRPYTTILMGIYFVLGLSSVGIFYCLIHRQVKRAAQ ALDQYKLRQASIHSNHVARTDEAMPGRFQELDSRLASGGPSEGISSEPVSAATTQTLEGDSSEVGDQINS KRAKQMAEKSPPEASAKAQPIKGARRAPDSSSEFGKVTRMCFAVFLCFALSYIPFLLLNILDARVQAPRV VHMLAANLTWLNGCINPVLYAAMNRQFRQAYGSILKRGPRSFHRLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020370 |
| ORF Size | 1188 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020370.3 |
| RefSeq Size | 1525 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 53831 |
| UniProt ID | Q9NQS5 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 43.7 kDa |
| Gene Summary | Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204621L1 | Lenti ORF clone of Human G protein-coupled receptor 84 (GPR84), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204621L2 | Lenti ORF clone of Human G protein-coupled receptor 84 (GPR84), mGFP tagged |
CNY 5,890.00 |
|
| RC204621L3 | Lenti ORF clone of Human G protein-coupled receptor 84 (GPR84), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204621L4 | Lenti ORF clone of Human G protein-coupled receptor 84 (GPR84), mGFP tagged |
CNY 5,890.00 |
|
| RG204621 | GPR84 (tGFP-tagged) - Human G protein-coupled receptor 84 (GPR84) |
CNY 5,256.00 |
|
| SC319759 | GPR84 (untagged)-Human G protein-coupled receptor 84 (GPR84) |
CNY 3,656.00 |
