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DNAJC25-GNG10 (NM_004125) Human Tagged ORF Clone
CAT＃: RC204572
- TrueORF®
DNAJC25 (Myc-DDK-tagged)-Human DNAJC25-GNG10 readthrough (DNAJC25-GNG10)
ORF Plasmid: tGFP
"NM_004125" in other vectors (5)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204572 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCGCCGCTGCTCTCTCCCGGCTGGGGAGCCGGGGCTGCCGGCCGGCGCTGGTGGATGCTGCTGG CGCCCCTGCTGCCGGCGCTGCTGCTGGTGCGGCCCGCGGGGGCCCTGGTGGAGGGGCTCTACTGCGGCAC GCGGGACTGCTACGAGGTGCTGGGCGTGAGCCGCTCGGCGGGCAAGGCGGAGATCGCGCGGGCCTACCGC CAGCTGGCCCGGCGCTACCACCCTGACCGCTACCGGCCCCAGCCCGGAGACGAGGGCCCCGGGCGGACGC CGCAGAGCGCCGAGGAGGCTTTCCTGCTGGTGGCAACCGCCTACGAGACACTCAAGGTCTCTCAGGCAGC TGCAGAGCTTCAACAGTACTGTATGCAGAATGCCTGCAAGGATGCCCTGCTGGTGGGTGTTCCAGCTGGA AGTAACCCCTTCCGGGAGCCTAGATCCTGTGCTTTACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204572 protein sequence
Red=Cloning site Green=Tags(s) MGAPLLSPGWGAGAAGRRWWMLLAPLLPALLLVRPAGALVEGLYCGTRDCYEVLGVSRSAGKAEIARAYR QLARRYHPDRYRPQPGDEGPGRTPQSAEEAFLLVATAYETLKVSQAAAELQQYCMQNACKDALLVGVPAG SNPFREPRSCALL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004125 |
| ORF Size | 459 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004125.4 |
| RefSeq Size | 1515 bp |
| RefSeq ORF | 462 bp |
| Locus ID | 552891 |
| UniProt ID | Q9H1X3 |
| Domains | DnaJ, G-gamma |
| Protein Families | Druggable Genome |
| MW | 16.5 kDa |
| Gene Summary | This gene represents naturally-occurring mRNAs that are co-transcribed products of the neighboring DNAJC25 and GNG10 genes. These transcripts include the first exon of DNAJC25 and the last two exons of GNG10, resulting in a protein that combines the N-terminus of DNAJC25 and the C-terminus of GNG10. [provided by RefSeq, Dec 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204572L3 | Lenti ORF clone of Human DNAJC25-GNG10 readthrough (DNAJC25-GNG10), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204572L4 | Lenti ORF clone of Human DNAJC25-GNG10 readthrough (DNAJC25-GNG10), mGFP tagged |
CNY 5,890.00 |
|
| RG204572 | DNAJC25 (tGFP-tagged) - Human DNAJC25-GNG10 readthrough (DNAJC25-GNG10) |
CNY 4,370.00 |
|
| SC117538 | DNAJC25 (untagged)-Human DNAJC25-GNG10 readthrough (DNAJC25-GNG10) |
CNY 3,760.00 |
|
| SC317252 | DNAJC25 (untagged)-Human DNAJC25-GNG10 readthrough (DNAJC25-GNG10) |
CNY 3,990.00 |
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