AP2 beta (TFAP2B) (NM_003221) Human Tagged ORF Clone

CAT#: RC204504

  • TrueORF®

TFAP2B (Myc-DDK-tagged)-Human transcription factor AP-2 beta (activating enhancer binding protein 2 beta) (TFAP2B)

ORF Plasmid: DDK tGFP



  "NM_003221" in other vectors (8)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-TFAP2B Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms AP-2B; AP-2beta; AP2-B; PDA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204504 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTCACCTCCTAGAGACCAGGCTGCCATCATGCTCTGGAAGCTTGTGGAGAATGTCAAGTACGAAG
ATATCTATGAGGACCGGCACGATGGTGTCCCGAGCCACAGCTCGCGGCTCTCCCAGCTGGGCTCGGTGTC
CCAAGGACCCTACTCGAGCGCCCCGCCGCTGTCCCACACCCCGTCGTCGGACTTCCAGCCGCCCTACTTC
CCACCCCCCTACCAGCCGCTCCCCTACCACCAGAGCCAGGACCCCTACTCCCACGTCAACGACCCCTACT
CCCTGAACCCACTGCACCAGCCCCAGCAACATCCCTGGGGGCAACGGCAGCGGCAAGAAGTGGGTTCGGA
AGCCGGCTCTCTCCTGCCCCAGCCTCGGGCCGCCTTGCCCCAGCTCTCGGGCCTTGACCCCCGGAGGGAC
TACCACTCGGTCCGCCGGCCGGACGTGCTGCTGCATTCGGCGCACCACGGCCTGGACGCGGGCATGGGTG
ACAGCCTCTCGCTGCACGGCCTCGGCCATCCCGGAATGGAAGACGTCCAGTCAGTTGAAGATGCCAATAA
CAGCGGCATGAATCTATTGGACCAGTCTGTCATTAAAAAAGTTCCAGTTCCTCCCAAATCGGTGACTTCT
CTAATGATGAATAAAGACGGCTTCCTGGGAGGCATGTCTGTCAACACCGGCGAGGTGTTTTGCTCCGTCC
CAGGCCGTTTGTCTCTGCTCAGTTCAACTTCGAAGTACAAAGTAACTGTGGGAGAAGTTCAGAGACGGCT
GTCGCCCCCTGAATGCCTCAATGCATCTCTCCTCGGCGGAGTCCTCAGAAGAGCCAAATCGAAAAATGGG
GGGAGATCTTTGCGAGAAAGGCTAGAAAAAATCGGTTTGAATTTACCCGCGGGCAGGCGCAAAGCAGCAA
ATGTCACGTTACTCACCTCCCTGGTGGAAGGAGAAGCTGTTCACTTAGCTAGGGATTTTGGGTACATTTG
CGAAACGGAGTTTCCCGCCAAAGCCGTCTCTGAGTATTTGAACCGGCAGCACACAGACCCGAGTGACCTG
CACTCCCGAAAGAATATGCTGTTGGCCACCAAGCAACTTTGTAAAGAATTTACGGATCTACTGGCGCAGG
ACCGGACACCGATAGGGAACAGCCGACCCAGCCCCATCCTGGAGCCGGGGATCCAGAGCTGCCTCACGCA
CTTCAGCCTCATCACGCACGGCTTCGGCGCCCCGGCCATTTGCGCCGCGCTCACGGCCCTGCAGAACTAT
CTCACCGAGGCGCTCAAAGGCATGGACAAGATGTTCTTGAACAACACCACCACTAACAGGCACACGTCTG
GGGAAGGCCCAGGTAGTAAAACTGGCGACAAGGAGGAGAAACACAGGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204504 protein sequence
Red=Cloning site Green=Tags(s)

MHSPPRDQAAIMLWKLVENVKYEDIYEDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDFQPPYF
PPPYQPLPYHQSQDPYSHVNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRD
YHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTS
LMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNG
GRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDL
HSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNY
LTEALKGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003221
ORF Size 1380 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003221.4
RefSeq Size 5770 bp
RefSeq ORF 1383 bp
Locus ID 7021
UniProt ID Q92481
Domains TF_AP-2
Protein Families Druggable Genome, Transcription Factors
MW 50.5 kDa
Gene Summary This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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