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KIAA0859 (METTL13) (NM_014955) Human Tagged ORF Clone
CAT＃: RC204397
- TrueORF®
METTL13 (Myc-DDK-tagged)-Human methyltransferase like 13 (METTL13), transcript variant 2
ORF Plasmid: tGFP
"NM_014955" in other vectors (5)
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CNY 7,030.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5630401D24Rik; CGI-01; DFNB26; DFNB26M; DFNM1; feat; KIAA0859; METTL13 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204397 representing NM_014955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCTTACCTAAAAGTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAGC GAGGAAAGAAAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACATAAATATATCAA GCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGTGAGCAACTGTATGATGTGGGC TATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTCATCAAGCAAATGAAGGAATGTAATGCCACCC GACGGCCCCAGATGAGCTTCTTGAAGATGGACATGACGCAGATGGAGTTTCCTGATGCCTCGTTCCAGGT GGTGTTGGACAAGGGCACCCTGGATGCTGTCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGAC AGGATGCTGGCTGAGGTTGGCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCAGG CTCACATCCTGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATGGTGAGGGTGCACCAAGTGGC CAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTCTCCTTGCCTGTCTTTGCCTTCATCATGACC AAGTTCAGGCCAGTCCCTGGCTCTGCCCTTCAGATCTTTGAGCTGTGTGCTCAGGAGCAGCGCAAGCCTG TGCGGCTGGAGAGTGCCGAGCGGCTGGCCGAGGCGGTGCAGGAGCGACAGCAGTATGCCTGGCTGTGCAG CCAGCTGCGCCGCAAGGCCAGGCTGGGGAGTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCA CGCTACACCCTCCACGTGGTGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTTGCGATTTTCA TCATCCCTCAGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGCCGGAAACAGCTGGCGGCCAG TGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGAGGTCAGCAGTATGAAAGCATGGACCACATC CAAGCTGAGCTGTCGGCTAGAGTCATGGAGCTGGCCCCAGCTGGGATGCCCACCCAGCAGCAGGTCCCCT TTCTGTCTGTGGGTGGGGACATTGGGGTCCGGACCGTTCAGCACCAAGACTGCAGCCCCTTGAGCGGTGA CTATGTCATTGAGGATGTGCAAGGGGATGACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGG AATGTGGTGCAGTCCGAAGCCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAGCGGAAAAAGG ACAGGAAGAAGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCGGGGCAGTCCATTGATAAGAG TTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCTGCTGAGAAACCCAGAGCTACTC CTAGAGATCCCACTGGCATTGTTGGTGGTAGGCCTGGGCGGGGGCAGCCTCCCCCTCTTTGTCCACGATC ATTTTCCAAAGTCCTGCATTGATGCTGTGGAGATCGATCCCTCCATGTTGGAAGTGGCCACCCAGTGGTT TGGCTTCTCCCAGAGTGACCGAATGAAGGTCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCA GGAGGAGGAGAAGCACGGCCTTGCTACGATGTCATAATGTTTGATGTTGACAGTAAGGACCCAACACTGG GAATGAGTTGTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAGGTTAAAAGCATCTTGACTCC TGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTGGGGCTAAAAGACTCAGTGCTGGCTGGGCTC AAGGCAGTGTTCCCCCTCCTATATGTCCGGCGAATTGAGGGTGAAGTGAATGAGATCCTGTTCTGTCAGC TGCACCCTGAGCAAAAACTTGCCACACCAGAGCTCCTAGAAACAGCCCAGGCTTTGGAGCGGACCCTGAG GAAGCCTGGGAGGGGTTGGGATGACACGTATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204397 representing NM_014955
Red=Cloning site Green=Tags(s) MNLLPKSSREFGSVDYWEKFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVIGCGNSELSEQLYDVG YRDIVNIDISEVVIKQMKECNATRRPQMSFLKMDMTQMEFPDASFQVVLDKGTLDAVLTDEEEKTLQQVD RMLAEVGRVLQVGGRYLCISLAQAHILKKAVGHFSREGWMVRVHQVANSQDQVLEAEPQFSLPVFAFIMT KFRPVPGSALQIFELCAQEQRKPVRLESAERLAEAVQERQQYAWLCSQLRRKARLGSVSLDLCDGDTGEP RYTLHVVDSPTVKPSRDNHFAIFIIPQGRETEWLFGMDEGRKQLAASAGFRRLITVALHRGQQYESMDHI QAELSARVMELAPAGMPTQQQVPFLSVGGDIGVRTVQHQDCSPLSGDYVIEDVQGDDKRYFRRLIFLSNR NVVQSEARLLKDVSHKAQKKRKKDRKKQRPADAEDLPAAPGQSIDKSYLCCEHHKAMIAGLALLRNPELL LEIPLALLVVGLGGGSLPLFVHDHFPKSCIDAVEIDPSMLEVATQWFGFSQSDRMKVHIADGLDYIASLA GGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGL KAVFPLLYVRRIEGEVNEILFCQLHPEQKLATPELLETAQALERTLRKPGRGWDDTYVLSDMLKTVKIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014955 |
| ORF Size | 2100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014955.1, NM_014955.2, NP_055770.1 |
| RefSeq Size | 3096 bp |
| RefSeq ORF | 1842 bp |
| Locus ID | 51603 |
| UniProt ID | Q8N6R0 |
| Protein Families | Druggable Genome |
| MW | 78.8 kDa |
| Gene Summary | Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed:30612740, PubMed:30143613). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed:30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed:30612740, PubMed:30143613). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed:30612740).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204397L3 | Lenti-ORF clone of METTL13 (Myc-DDK-tagged)-Human methyltransferase like 13 (METTL13), transcript variant 2 |
CNY 8,930.00 |
|
| RC204397L4 | Lenti-ORF clone of METTL13 (mGFP-tagged)-Human methyltransferase like 13 (METTL13), transcript variant 2 |
CNY 8,930.00 |
|
| RG204397 | METTL13 (tGFP-tagged) - Human methyltransferase like 13 (METTL13), transcript variant 2 |
CNY 7,700.00 |
|
| SC121821 | METTL13 (untagged)-Human methyltransferase like 13 (METTL13), transcript variant 2 |
CNY 4,576.00 |
|
| SC323939 | METTL13 (untagged)-Human methyltransferase like 13 (METTL13), transcript variant 2 |
CNY 4,576.00 |
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