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TCP1 epsilon (CCT5) (NM_012073) Human Tagged ORF Clone
CAT＃: RC204358
CCT5 (Myc-DDK-tagged)-Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5)
ORF Plasmid: tGFP
"NM_012073" in other vectors (6)
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CNY 6,040.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CCT-epsilon; CCTE; HEL-S-69; PNAS-102; TCP-1-epsilon |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204358 representing NM_012073.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGTCCATGGGGACCCTCGCCTTCGATGAATATGGGCGCCCTTTCCTCATCATCAAGGATCAGGAC CGCAAGTCCCGTCTTATGGGACTTGAGGCCCTCAAGTCTCATATAATGGCAGCAAAGGCTGTAGCAAAT ACAATGAGAACATCACTTGGACCAAATGGGCTTGATAAGATGATGGTGGATAAGGATGGGGATGTGACT GTAACTAATGATGGGGCCACCATCTTAAGCATGATGGATGTTGATCATCAGATTGCCAAGCTGATGGTG GAACTGTCCAAGTCTCAGGATGATGAAATTGGAGATGGAACCACAGGAGTGGTTGTCCTGGCTGGTGCC TTGTTAGAAGAAGCGGAGCAATTGCTAGACCGAGGCATTCACCCAATCAGAATAGCCGATGGCTATGAG CAGGCTGCTCGCGTTGCTATTGAACACCTGGACAAGATCAGCGATAGCGTCCTTGTTGACATAAAGGAC ACCGAACCCCTGATTCAGACAGCAAAAACCACGCTGGGCTCCAAAGTGGTCAACAGTTGTCACCGACAG ATGGCTGAGATTGCTGTGAATGCCGTCCTCACTGTAGCAGATATGGAGCGGAGAGACGTTGACTTTGAG CTTATCAAAGTAGAAGGCAAAGTGGGCGGCAGGCTGGAGGACACTAAACTGATTAAGGGCGTGATTGTG GACAAGGATTTCAGTCACCCACAGATGCCAAAAAAAGTGGAAGATGCGAAGATTGCAATTCTCACATGT CCATTTGAACCACCCAAACCAAAAACAAAGCATAAGCTGGATGTGACCTCTGTCGAAGATTATAAAGCC CTTCAGAAATACGAAAAGGAGAAATTTGAAGAGATGATTCAACAAATTAAAGAGACTGGTGCTAACCTA GCAATTTGTCAGTGGGGCTTTGATGATGAAGCAAATCACTTACTTCTTCAGAACAACTTGCCTGCGGTT CGCTGGGTAGGAGGACCTGAAATTGAGCTGATTGCCATCGCAACAGGAGGGCGGATCGTCCCCAGGTTC TCAGAGCTCACAGCCGAGAAGCTGGGCTTTGCTGGTCTTGTACAGGAGATCTCATTTGGGACAACTAAG GATAAAATGCTGGTCATCGAGCAGTGTAAGAACTCCAGAGCTGTAACCATTTTTATTAGAGGAGGAAAT AAGATGATCATTGAGGAGGCGAAACGATCCCTTCACGATGCTTTGTGTGTCATCCGGAACCTCATCCGC GATAATCGTGTGGTGTATGGAGGAGGGGCTGCTGAGATATCCTGTGCCCTGGCAGTTAGCCAAGAGGCG GATAAGTGCCCCACCTTAGAACAGTATGCCATGAGAGCGTTTGCCGACGCACTGGAGGTCATCCCCATG GCCCTCTCTGAAAACAGTGGCATGAATCCCATCCAGACTATGACCGAAGTCCGAGCCAGACAGGTGAAG GAGATGAACCCTGCTCTTGGCATCGACTGTTTGCACAAGGGGACAAATGATATGAAGCAACAGCATGTC ATAGAAACCTTGATTGGCAAAAAGCAACAGATATCTCTTGCAACACAAATGGTTAGAATGATTTTGAAG ATTGATGACATTCGTAAGCCTGGAGAATCTGAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC204358
Blue=ORF Red=Cloning site Green=Tag(s) MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVT VTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYE QAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFE LIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKA LQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRF SELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIR DNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVK EMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC204358 also available, TP304358M |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012073 |
| ORF Size | 1623 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012073.2 |
| RefSeq Size | 3403 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 22948 |
| UniProt ID | P48643 |
| Domains | cpn60_TCP1 |
| Protein Families | Druggable Genome |
| MW | 59.7 kDa |
| Gene Summary | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204358L1 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204358L2 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5), mGFP tagged |
CNY 8,440.00 |
|
| RC204358L3 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204358L4 | Lenti ORF clone of Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5), mGFP tagged |
CNY 5,890.00 |
|
| RG204358 | CCT5 (tGFP-tagged) - Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5) |
CNY 7,640.00 |
|
| SC319578 | CCT5 (untagged)-Human chaperonin containing TCP1, subunit 5 (epsilon) (CCT5) |
CNY 4,040.00 |
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