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PHF21B (NM_138415) Human Tagged ORF Clone
CAT＃: RC204284
PHF21B (Myc-DDK-tagged)-Human PHD finger protein 21B (PHF21B), transcript variant 1
ORF Plasmid: tGFP
"NM_138415" in other vectors (4)
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CNY 5,928.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BHC80L; PHF4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204284 representing NM_138415
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGCAGAGCCGGCCCGAGGCGCTCGCCGTGGAACTCGCGCGCCACCAGAACGGCGACCTCAAGA AGCAGCTCCACGAAAGGCAGCCGCGGATCGCCGCGCTCAGCGACAAACAAGCTTTGGGAACGATCACTGC AGTGCCTGTCACGGGTCCTCAGGTCAGCTCCTTGCAGAGGTTGGCCGGGCAAGGAGCGGCAGTGCTACCT CAGGTTAGGCCAAAGACTCTGATTCCAGACAGCCTCCCCGTTGCCCCGGGCCGGGACCGGCCACCCAAGC AGCCCCCAACATTCCAGAAGGCCACCGTGGTCAGCGTCAAGAACCCCAGCCCAGCCCTCCCCACCGCCAA CAACACTGTCAGCCATGTGCCAGCGCCCGGCAGCCAGCCCCAGGCCCTCGCCGAGCCCGCCGCCCTCGCC TCTCCGCTGAGCAGTGCGGGGGTGGCCTACGCCATCATCTCCACCTCCCCCAGCAATGCCGCCGCCATGG CCCCCAGCACCGCCGTGTCTGTGGTCAGTGACAGCATCAAAGTCCAGCCCCTCCTCATCAGTGCTGACAA CAAGCCTCCTCCACGCCTCCTCTCTTCCCCTCACCCCGCAACCCATCACTGTCCCCTCCACCCCTCCTCT CTTCCCCTCACCCCTCCCTCCCCATCACTGTCCCCTTCACCCCTCCATGGCATCTTCCAGGTCATCATCA TTCAGCCTCAAGTGCAGACGCAGCCCGAGAGCACGGCAGAGTCGCGGCCGCCCACAGAGGAGCCATCTCA GGGAGCTCAGGCCACCAAAAAGAAGAAGGAAGACCGGCCCCCGACCCAGGAGAACCCCGAGAAAATCGCC TTCATGGTAGCGCTAGGCCTGGTTACCACGGAACATTTGGAAGAAATCCAGAGCAAGCGACAGGAGCGGA AGAGAAGAAGCACAGCCAACCCTGCCTACAGCGGCCTCCTGGAGACCGAGAGGAAACGGCTGGCCTCCAA CTATCTCAACAACCCCCTGTTCCTCACAGCGAGAGCCAATGAGGACCCCTGCTGGAAGAACGAGATCACC CACGATGAGCACTGTGCCGCCTGCAAGCGAGGGGCCAACCTGCAGCCCTGCGGCACCTGCCCGGGGGCCT ACCACCTCAGCTGCCTGGAGCCGCCCCTCAAGACGGCGCCCAAGGGCGTGTGGGTGTGCCCCAGGTGCCA GCAGAAGGCCTTAAAGAAAGACGAGGGTGTGCCCTGGACTGGGATGCTGGCCATCGTGCACTCTTATGTC ACCCACAAGACAGTCAAAGAAGAGGAGAAGCAGAAGCTGCTGCAACGAGGCAGTGAGCTGCAGAACGAGC ACCAGCAGCTGGAGGAGCGGGACCGGCGGCTGGCGTCAGCAGTGCAGAAATGCCTGGAGTTGAAGACAAG CCTGCTGGCCCGCCAGAGGGGCACCCAGTCATCCCTGGACCGCCTGCGGGCCCTCCTGAGACTGATACAG GGCGAGCAGCTGCTCCAGGTCACCATGACGACCACTAGCCCTGCCCCACTGCTGGCCGGGCCCTGGACCA AGCCCTCAGTGGCAGCCACACACCCCACCGTCCAGCACCCCCAGGGCCACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204284 representing NM_138415
Red=Cloning site Green=Tags(s) MELQSRPEALAVELARHQNGDLKKQLHERQPRIAALSDKQALGTITAVPVTGPQVSSLQRLAGQGAAVLP QVRPKTLIPDSLPVAPGRDRPPKQPPTFQKATVVSVKNPSPALPTANNTVSHVPAPGSQPQALAEPAALA SPLSSAGVAYAIISTSPSNAAAMAPSTAVSVVSDSIKVQPLLISADNKPPPRLLSSPHPATHHCPLHPSS LPLTPPSPSLSPSPLHGIFQVIIIQPQVQTQPESTAESRPPTEEPSQGAQATKKKKEDRPPTQENPEKIA FMVALGLVTTEHLEEIQSKRQERKRRSTANPAYSGLLETERKRLASNYLNNPLFLTARANEDPCWKNEIT HDEHCAACKRGANLQPCGTCPGAYHLSCLEPPLKTAPKGVWVCPRCQQKALKKDEGVPWTGMLAIVHSYV THKTVKEEEKQKLLQRGSELQNEHQQLEERDRRLASAVQKCLELKTSLLARQRGTQSSLDRLRALLRLIQ GEQLLQVTMTTTSPAPLLAGPWTKPSVAATHPTVQHPQGHN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138415 |
| ORF Size | 1593 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138415.5 |
| RefSeq Size | 3694 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 112885 |
| UniProt ID | Q96EK2 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 57.3 kDa |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PHF21B as a candidate tumor suppressor gene in head and neck squamous cell carcinomas
,null,
Molecular Oncology
,PubMed ID 25454821
[PHF21B]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204284L3 | Lenti ORF clone of Human PHD finger protein 21B (PHF21B), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204284L4 | Lenti ORF clone of Human PHD finger protein 21B (PHF21B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG204284 | PHF21B (tGFP-tagged) - Human PHD finger protein 21B (PHF21B), transcript variant 1 |
CNY 7,528.00 |
|
| SC123134 | PHF21B (untagged)-Human PHD finger protein 21B (PHF21B), transcript variant 1 |
CNY 5,944.00 |
