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TRAK1 (NM_014965) Human Tagged ORF Clone
CAT＃: RC204282
TRAK1 (Myc-DDK-tagged)-Human trafficking protein, kinesin binding 1 (TRAK1), transcript variant 2
ORF Plasmid: tGFP
"NM_014965" in other vectors (4)
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CNY 5,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EIEE68; MILT1; OIP106 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC204282 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGCGAGACAAGGGCGGGGAAGAAGAATGTTTTGAATACGACTGCCAGGATGAAGAGAGGAAGC CAACCCACAGGCAGCATGACACCCAGGACCTCTTGGAAGAGGTTTTATGTGCTGAAAGAGTTGGCCAGAT GACTAAGACATATAATGACATAGATGCTGTCACTCGGCTTCTTGAGGAGAAAGAGCGGGATTTAGAATTG GCCGCTCGCATCGGCCAGTCGTTGTTGAAGAAGAACAAGACCCTAACCGAGAGGAACGAGCTGCTGGAGG AGCAGGTGGAACACATCAGGGAGGAGGTGTCTCAGCTCCGGCATGAGCTGTCCATGAAGGATGAGCTGCT TCAGTTCTACACCAGCGCTGCGGAGGAGAGTGAGCCCGAGTCCGTTTGCTCAACCCCGTTGAAGAGGAAT GAGTCGTCCTCCTCAGTCCAGAATTACTTTCATTTGGATTCTCTTCAAAAGAAGCTGAAAGACCTTGAAG AGGAGAATGTTGTACTTCGATCCGAGGCCAGCCAGCTGAAGACAGAGACCATCACCTATGAGGAGAAGGA GCAGCAGCTGGTCAATGACTGCGTGAAGGAGCTGAGGGATGCCAATGTCCAGATTGCTAGTATCTCAGAG GAACTGGCCAAGAAGACGGAAGATGCTGCCCGCCAGCAAGAGGAGATCACACACCTGCTATCGCAAATAG TTGATTTGCAGAAAAAGGCAAAAGCTTGCGCAGTGGAAAATGAAGAACTTGTCCAGCATCTGGGGGCTGC TAAGGATGCCCAGCGGCAGCTCACAGCCGAGCTGCGTGAGCTGGAGGACAAGTACGCAGAGTGCATGGAG ATGCTGCATGAGGCGCAGGAGGAGCTGAAGAACCTCCGGAACAAAACCATGCCCAATACCACGTCTCGGC GCTACCACTCGCTGGGCCTGTTTCCCATGGATTCCTTGGCAGCAGAGATTGAGGGAACGATGCGCAAGGA GCTGCAGTTGGAAGAGGCCGAGTCTCCAGACATCACTCACCAGAAGCGTGTCTTTGAGACAGTAAGAAAC ATCAACCAGGTTGTCAAGCAGAGATCTCTGACCCCTTCTCCCATGAACATCCCCGGCTCCAACCAGTCCT CGGCCATGAACTCCCTCCTGTCCAGCTGCGTCAGCACCCCCCGGTCCAGCTTCTACGGCAGCGACATAGG CAACGTCGTCCTCGACAACAAGACCAACAGCATCATTCTGGAAACAGAGGCAGCCGACCTGGGAAACGAT GAGCGGAGTAAGAAGCCGGGGACGCCGGGCACCCCAGGCTCCCACGACCTGGAGACGGCGCTGAGGCGGC TGTCCCTGCGCCGGGAGAACTACCTCTCGGAGAGGAGGTTCTTTGAGGAGGAGCAAGAGAGGAAGCTCCA GGAGCTGGCGGAGAAGGGCGAGCTGCGCAGCGGCTCCCTCACACCCACTGAGAGCATCATGTCCCTGGGC ACGCACTCCCGCTTCTCCGAGTTCACCGGCTTCTCTGGCATGTCCTTCAGCAGCCGCTCCTACCTGCCTG AGAAGCTCCAGATCGTGAAGCCGCTGGAAGGTTCTGCCACACTTCACCACTGGCAGCAGTTGGCCCAACC TCACCTTGGGGGCATCCTGGACCCCCGGCCTGGTGTGGTCACCAAGGGCTTCCGGACGCTGGATGTTGAC CTGGACGAAGTGTACTGCCTTAACGACTTTGAAGAAGATGACACAGGTGACCACATTTCTCTCCCACGCC TAGCTACCTCCACTCCAGTTCAGCACCCAGAGACCTCAGGTGAGAGGTCCCAAGCACGTGTGACTGTCTC AGGCAGCAGAAGTTACCCGAGCCGGCCTCAGGCTTCCCCAGAGGAGATGCAGGAGCCGCCAGCGGCCACG GAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGGGTCTGGTGAGGGCACCACGATAAGTCCTGTAA ACTTGGCACCTTTCCCGGAGGCAGAGTTTTGGGCCATTCTCACCTCTGTTCCAGGCACCATCCGTAGTGG TTCTCTGTCTGTAGCTTCCGCTCGTCTGTGTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204282 protein sequence
Red=Cloning site Green=Tags(s) MSLRDKGGEEECFEYDCQDEERKPTHRQHDTQDLLEEVLCAERVGQMTKTYNDIDAVTRLLEEKERDLEL AARIGQSLLKKNKTLTERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTPLKRN ESSSSVQNYFHLDSLQKKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVKELRDANVQIASISE ELAKKTEDAARQQEEITHLLSQIVDLQKKAKACAVENEELVQHLGAAKDAQRQLTAELRELEDKYAECME MLHEAQEELKNLRNKTMPNTTSRRYHSLGLFPMDSLAAEIEGTMRKELQLEEAESPDITHQKRVFETVRN INQVVKQRSLTPSPMNIPGSNQSSAMNSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGND ERSKKPGTPGTPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESIMSLG THSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEGSATLHHWQQLAQPHLGGILDPRPGVVTKGFRTLDVD LDEVYCLNDFEEDDTGDHISLPRLATSTPVQHPETSGERSQARVTVSGSRSYPSRPQASPEEMQEPPAAT EEEEEEEEEEEEGSGEGTTISPVNLAPFPEAEFWAILTSVPGTIRSGSLSVASARLCG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014965 |
| ORF Size | 2064 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014965.5 |
| RefSeq Size | 4623 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 22906 |
| UniProt ID | Q9UPV9 |
| Domains | HAP1_N |
| Protein Families | Transcription Factors |
| MW | 77.5 kDa |
| Gene Summary | Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed:24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204282L3 | Lenti ORF clone of Human trafficking protein, kinesin binding 1 (TRAK1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204282L4 | Lenti ORF clone of Human trafficking protein, kinesin binding 1 (TRAK1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG204282 | TRAK1 (tGFP-tagged) - Human trafficking protein, kinesin binding 1 (TRAK1), transcript variant 2 |
CNY 6,640.00 |
|
| SC319663 | TRAK1 (untagged)-Human trafficking protein, kinesin binding 1 (TRAK1), transcript variant 2 |
CNY 5,032.00 |
